Cytotoxicity of some Cameroonian spices and selected medicinal plant extracts

Kuete, Victor; Krusche, Benjamin; Youns, Mаhmoud; Voukeng, Igor K.; Fankam, Aimé G.; Tankeo, Simplice B.; Lacmata, Stephen Tamekou; Efferth, Thomas

doi:10.1016/j.jep.2011.01.035

Cited by 166 publications

(118 citation statements)

References 82 publications

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“…The observed cytotoxic effect of ICL extract only to the SCC-9 squamous cell carcinoma cells and not to the NIH/3T3 normal fibroblast cells indicates that the cytotoxicity is restricted only to the cancer cells and not to the normal cells ( Figure 2C). Our data on the cancer cell specific cytotoxicity of ICL extract is in agreement with the previous reports which have demonstrated cytotoxicity of I. cylindrica extract in vitro on other cancer cell lines (Kuete et al, 2011;Kuete et al, 2013).…”

Section: Discussionsupporting

confidence: 93%

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Anti-Cancer Effects of Imperata cylindrica Leaf Extract on Human Oral Squamous Carcinoma cell line SCC-9 in Vitro

Keshava¹,

Muniyappa²,

Gope³

et al. 2016

Asian Pacific Journal of Cancer Prevention

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Imperata cylindrica, a tall tufted grass which has multiple pharmacological applications is one of the key ingredients in various traditional medicinal formula used in India. Previous reports have shown that I. cylindrica plant extract inhibited cell proliferation and induced apoptosis in various cancer cell lines. To our knowledge, no studies have been published on the effect of I. cylindrica leaf extract on human oral cancers. The present study was undertaken in order to evaluate the anticancer properties of the leaf extract of I. cylindrica using an oral squamous cell carcinoma cell line SCC-9 as an in vitro model system. A methanol extract from dried leaves of I. cylindrica (ICL) was prepared by standard procedures. Effects of the ICL extract on the morphology of SCC-9 cells was visualized by microscopy. Cytotoxicity was determined by MTT assay. Effects of the ICL extract on colony forming ability of SCC-9 cells was evaluated using clonogenic assay. Cell cycle analysis was performed by flow cytometry and induction of apoptosis was determined by DNA fragmentation assay. The ICL extract treatment caused cytotoxicity and induced cell death in vitro in SCC-9 cells in a dose-dependent manner. This treatment also significantly reduced the clonogenic potential and inhibited cell proliferation by arresting the cell cycle in the G2/M phase. Furthermore, DNA fragmentation assays showed that the observed cell death was caused by apoptosis. This is the first report showing the anticancer activity of the methanol extracts from the leaves of I. cylindrica in human oral cancer cell line. Our data indicates that ICL extract could be considered as one of the lead compounds for the formulation of anticancer therapeutic agents to treat/manage human oral cancers. The natural abundance of I. cylindrica and its wide geographic distribution could render it one of the primary resource materials for preparation of anticancer therapeutic agents.

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Section: Discussionsupporting

confidence: 93%

“…Previous reports have shown that I. cylindrica plant extract inhibited cell proliferation and induced apoptosis in various cancer cell lines (Kuete et al, 2011;Kuete et al, 2013). To our knowledge, so far, no studies have been published on the effect of I. cylindrica leaf extract on human oral cancers.…”

Section: Introductionmentioning

confidence: 91%

Anti-Cancer Effects of Imperata cylindrica Leaf Extract on Human Oral Squamous Carcinoma cell line SCC-9 in Vitro

Keshava¹,

Muniyappa²,

Gope³

et al. 2016

Asian Pacific Journal of Cancer Prevention

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“…As medicine, in rural communities, where villagers have hardly access to health centres, the use of bark and seeds to treat diarrhoea, abdominal pains, flatulence, constipation, gastralgia, gastritis or stomach ulcers (digestive system) was also reported throughout the Centre and South regions of Cameroon (Nyemba et al, 1990;Kuete et al, 2011), at Douala in the Littoral region (Dibong et al, 2013;Ngene et al 2015) and at Bangangte in the West region (Noumi & Dibakto, 2000). The bark and seeds enjoy a folk reputation as antidote to poisoning and snake bites (Specific diseases and conditionsmiscellaneous), and as aphrodisiac or stimulant (Effects on reproductive system, in relation with glandular systems) not only in the Bipindi-Akom II region, but also in Douala (Dibong et al, 2013;Ngene et al, 2015), Yaoundé, Kribi (Adjanohoun et al, 1996), and throughout Cameroon, especially in the South and Central regions (Kamanyi et al, 1990;Nyemba et al 1990;Vivien & Faure, 1996;Ndoye et al 1999;Sunderland et al, 1999;Kuete et al, 2011). The use of leaves against evil spirits or to chase away ghosts (Directions for medico-magic use) and as insect repellent (Physiological effects on parasite or pathogenic organisms), mentioned by elderly key-informants of the Bipindi-Akom II region, suggests that this type of ethnobotanical use was applied in the past.…”

Section: Resultsmentioning

confidence: 99%

<i>Garcinia lucida</i> Vesque (Clusiaceae): from traditional uses to pharmacopeic monograph for an emerging local plant-based drug development

Guedje¹,

Tadjouteu²,

Onana³

et al. 2017

J. App. Bioscience.

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Guedje et al., J. Appl. Biosci. 2017 Garcinia lucida Vesque (Clusiaceae): from traditional uses to pharmacopeic monograph for an emerging local plant-based drug development 10594Journal of Applied Biosciences 109: 10594-10608 ISSN 1997-5902 Garcinia lucida Vesque (Clusiaceae): from traditional uses to pharmacopeic monograph for an emerging local plant-based drug development ABSTRACT Objectives: This paper seeks to assess information on the identity, traditional uses, safety and efficacy of Garcinia lucida Vesque (Essok in Boulou and Ewondo local language), in order to highlight it potential as species to be registered in medicinal plant list and formularies needed for the development of monographs leading to local production of phytomedicines. Methodology and Results: Ethnobotanical survey was carried in the Bipindi-Akom II region (South Cameroon) with the help of interviews based on standardised questionnaires addressed to key-informants. Bark and seeds were the major parts used, highly appreciated due to its properties in preventing consumers from poison, diarrhoea and headaches. The most important therapeutic indications were additive to palm wine, antidote to poisoning, gastritis and snake bite. Other uses in treating included gynaecological pains and infections, sexual diseases and cancers. The bark was also believed by local people to act on the stability of chemical antibiotic drugs in pharmacy, while leaves were used as insect repellent against mosquitoes and cockroaches. Conclusions and application of findings: As an additive in palm wine processing, poisoning antidote, aphrodisiac and medicines, bark and seeds are widely used in the Bipindi-Lolodorf-Akom II area, in the Centre and South regions of Cameroon, in Gabon and Equatorial Guinea, in Central Africa and West Africa. This similarity of use across many regions is considered as important biological activity marker tools guiding plant parts or species selection in drug discovery and development using ethnopharmacological approach. Through the ethnobotanical and biological tests, assessed, accurate information is provided to substantiate safety and efficacy of G. lucida and to satisfy the requirements of official compendia such as pharmacopoeia. Further studies are needed to understand the effects of bark and seeds as additive to palm wine, as well as the interaction between bark and chemical antibiotic drugs.

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“…Hopefully, this will lead to new information on plant applications and new perspective on the potential use of plant-derived products (Mouhssen 2004). In Cameroon, there are many dietary spices that are used by traditional healers to cure several diseases such as cancer and microbial infections (Tekwu et al 2012;Kuete et al 2011). Among them, Aframomum daniellii (Hook.…”

Section: Introductionmentioning

confidence: 99%

Traditional herbal remedies and dietary spices from Cameroon as novel sources of larvicides against filariasis mosquitoes?

et al. 2016

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In Cameroon, many dietary spices are used by traditional healers to cure several diseases such as cancer and microbial infections. Aframomum daniellii, Dichrostachys cinerea and Echinops giganteus are Cameroonian spices widely used as flavourings and as food additives. Moreover, they are traditionally herbal remedies employed to treat several diseases, as well as to control populations of insect pests. In this research, we analysed the chemical composition of A. daniellii, D. cinerea and E. giganteus essential oils and we evaluated their larvicidal potential against larvae of the filariasis and West Nile virus vector Culex quinquefasciatus. The essential oils were obtained from different plant parts by hydrodistillation and their composition was analysed by GC-MS. The three spices exhibited different volatile chemical profiles, being characterized by 1,8-cineole, sabinene and β-pinene (A. daniellii), geraniol and terpinen-4-ol (D. cinerea), and silphiperfol-6-ene and presilphiperfolan-8-ol (E. giganteus). Results showed that the highest larvicidal toxicity on Cx. quinquefasciatus was exerted by D. cinerea essential oil (LC 50 = 39.1 μL L −1 ), followed by A. daniellii ) and E. giganteus (LC 50 = 227.4 μL L −1 ). Overall, the chance to use the D. cinerea essential oil against Cx. quinquefasciatus young instars seems promising, since it is effective at moderate doses and could be an advantageous alternative to build newer mosquito control tools.

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Cytotoxicity of some Cameroonian spices and selected medicinal plant extracts

Cited by 166 publications

References 82 publications

Anti-Cancer Effects of Imperata cylindrica Leaf Extract on Human Oral Squamous Carcinoma cell line SCC-9 in Vitro

Anti-Cancer Effects of Imperata cylindrica Leaf Extract on Human Oral Squamous Carcinoma cell line SCC-9 in Vitro

<i>Garcinia lucida</i> Vesque (Clusiaceae): from traditional uses to pharmacopeic monograph for an emerging local plant-based drug development

Traditional herbal remedies and dietary spices from Cameroon as novel sources of larvicides against filariasis mosquitoes?

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