Cytotoxicity of Probiotics from Philippine Commercial Dairy Products on Cancer Cells and the Effect on Expression ofcfosandcjunEarly Apoptotic-Promoting Genes andInterleukin-1βandTumor Necrosis Factor-αProinflammatory Cytokine Genes

Shyu, Peter; Oyong, Glenn G.; Cabrera, Esperanza C.

doi:10.1155/2014/491740

Cited by 32 publications

(20 citation statements)

References 27 publications

(25 reference statements)

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“…Several studies have revealed a number of beneficial effects of certain lactobacilli, such as the suppression of pathogenic bacteria in the gut and inhibition of allergic, inflammatory and neoplastic changes ( 38 – 41 ). Furthermore, it has been shown that lactobacilli are particularly useful in promoting gastric ulcer healing in rats, when administered as an individual probiotic strain, such as Lactobacillus rhamnosus GG ( 42 ), Lactobacillus gasseri OLL2716 ( 43 , 44 ), or Lactobacillus acidophilus ( 45 , 46 ) or as a probiotic mixture, VSL#3 ( 47 ).…”

Section: Probioticsmentioning

confidence: 99%

Potential role of probiotics in the management of gastric ulcer

Khoder

Al-Menhali

Al-Yassir

et al. 2016

Experimental and Therapeutic Medicine

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Gastric ulcer is one of the most common chronic gastrointestinal diseases characterized by a significant defect in the mucosal barrier. Helicobacter pylori (H. pylori) infection and the frequent long-term use of non-steroidal anti-inflammatory drugs are major factors involved in gastric ulcer development. Acid inhibitors and antibiotics are commonly used to treat gastric ulcer. However, in the last few decades, the accumulating evidence for resistance to antibiotics and the side effects of antibiotics and acid inhibitors have drawn attention to the possible use of probiotics in the prevention and treatment of gastric ulcer. Probiotics are live microorganisms that when administered in adequate amounts confer health benefits on the host. Currently, the available experimental and clinical studies indicate that probiotics are promising for future applications in the management of gastric ulcers. This review aims to provide an overview of the general health benefits of probiotics on various systemic and gastrointestinal disorders with a special focus on gastric ulcer and the involved cellular and molecular mechanisms: i) Protection of gastric mucosal barrier; ii) upregulation of prostaglandins, mucus, growth factors and anti-inflammatory cytokines; iii) increased cell proliferation to apoptosis ratio; and iv) induction of angiogenesis. Finally, some of the available data on the possible use of probiotics in H. pylori eradication are discussed.

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Section: Probioticsmentioning

confidence: 99%

Potential role of probiotics in the management of gastric ulcer

Khoder

Al-Menhali

Al-Yassir

et al. 2016

Experimental and Therapeutic Medicine

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“…Studies have shown the pro‐apoptotic effects of Lactobacillus species on different cancer cells (Choi et al ). Shyu et al demonstrated that the probiotic supernatants up‐regulated the apoptosis gene expression in HT‐29 and HCT116 cells (Shyu et al ). Riaz et al verified that probiotic Lactobacillus strains isolated from human breast milk have anticancer properties on HeLa cells by an increase in expression of the apoptotic gene (Riaz Rajoka et al ).…”

Section: Resultsmentioning

confidence: 99%

Potential anticancer effects of cell wall protein fractions from Lactobacillus paracasei on human intestinal Caco‐2 cell line

Nozari

Faridvand

Etesami

et al. 2019

Lett Appl Microbiol

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Consumption of probiotics has an important role in colorectal cancer prevention. In this study, we aimed to explore that the cell wall protein fractions from Lactobacillus paracasei could induce apoptosis on Caco‐2 cell line. The cell wall proteins from L. paracasei were fractionated by gel filtration chromatography (F1, F2 and F3) and characterized by polyacrylamide gel electrophoresis (SDS‐PAGE). The anticancer properties were evaluated using MTT assay and Annexin V‐FITC/PI staining. Administration of L. paracasei increased a significant concentration‐ and time‐dependent anti‐proliferative effect on Caco‐2 cell line, determined by cell viability assays. However, a dramatic decrease in cell viability of Caco‐2 cells was observed at the concentration of 100 µg ml−1 of F1 L. paracasei for 72 h (58% cell viability, P < 0·05) The results showed that F1 L. paracasei could induce apoptosis in Caco‐2 cancer cell line by increased in annexin V and propidium iodide staining for 72 h (up to 90·6%, P < 0·001). These results indicated the importance of the anticancer effects of cell wall protein fractions of L. paracasei in human colon carcinoma Caco‐2 cell line. Thus, cell wall protein fractions of L. paracasei can be a potential chemotherapeutic agent against Caco‐2 cell lines. Significance and Impact of the Study Significance and Impact of the Study: Our findings revealed that the newly identified cell wall protein fractions from probiotic Lactobacillus paracasei inhibit the cell growth of human colon carcinoma cell line (Caco‐2), and the results indicated that the cell wall proteins from L. paracasei can be a potential chemotherapeutic agent against Caco‐2 cell lines.

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“…isolated from Yakult did not have a significant cytotoxic effect on normal human dermal fibroblasts and THP-1 leukemia cells but were significantly cytotoxic for the HT-29 and HCT116 colon cancer cell lines. [21] When these probiotics are taken in specified doses, these have health benefits beyond the intrinsic basic nutritional contents. [22] As such, several criteria for these probiotics have been established such as the potential to resist chemical processes (bile and gastric acids), to influence metabolic activities, to persist and survive in the human gut, and to modulate the immune response.…”

Section: Discussionmentioning

confidence: 99%

Viability kinetics of Lactobacillus casei Shirota strain in a commercial fermented milk drink during refrigerated storage

Sumalapao¹,

Mesina²,

Cabrera³

et al. 2017

Natl J Physiol Pharm Pharmacol

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Background: One of the most important parameters in a commercial fermented milk drink containing probiotics is the viability of the microorganisms. Aims and Objectives: This study described the viability kinetics of Lactobacillus casei Shirota strain in a commercial fermented milk drink during refrigerated storage using nonlinear models. Materials and Methods: Viability of L. casei Shirota strain was monitored during refrigerated storage using standard bacterial plate count method. Several nonlinear mathematical models such as the zero-order, first-order, and second-order kinetic equations were employed in describing the population dynamics. Best-fit models were selected based on prescribed criteria including sum of squares of the error, p-value, and coefficient of determination. Results: The viable counts of L. casei Shirota strain in the fermented milk samples stored under refrigerated conditions decreased from 3.73 × 10 8 colony forming units per ml (CFU/ml) to 2.70 × 10 8 CFU/ml when monitored every 3-4 days interval over 10 different monitoring points within the product's indicated shelf life. The counts significantly differed between the monitoring points that were at least 14 days apart. The lowest viable count was still within the recommended therapeutic dose. The decrease in the bacterial population behaved under a second-order kinetic relationship. Conclusion: The viability of L. casei Shirota strain in a commercial fermented milk product during refrigerated storage is governed in accordance with the second-order kinetic mechanism.

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Cytotoxicity of Probiotics from Philippine Commercial Dairy Products on Cancer Cells and the Effect on Expression ofcfosandcjunEarly Apoptotic-Promoting Genes andInterleukin-1βandTumor Necrosis Factor-αProinflammatory Cytokine Genes

Cited by 32 publications

References 27 publications

Potential role of probiotics in the management of gastric ulcer

Potential role of probiotics in the management of gastric ulcer

Potential anticancer effects of cell wall protein fractions from Lactobacillus paracasei on human intestinal Caco‐2 cell line

Viability kinetics of Lactobacillus casei Shirota strain in a commercial fermented milk drink during refrigerated storage

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