Potential anticancer effects of cell wall protein fractions from
            <i>Lactobacillus paracasei</i>
            on human intestinal Caco‐2 cell line

Nozari, Samira; Faridvand, Yousef; Etesami, Alireza; Beiki, M. Ahmad Khan; Mazrakhondi, Seyed Ali Miresmaeili; Abdolalizadeh, Jalal

doi:10.1111/lam.13198

Cited by 21 publications

(7 citation statements)

References 31 publications

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“…Due to the different mechanism of action, several publications applied both methods to investigate cytotoxicity of different chemicals [28][29][30]. In order to further study cytotoxic properties of PEGs, we used labelled annexin V and propidium iodide to discriminate between necrotic and early apoptotic cells with flow cytometry [29,31,32]. Autophagosome formation was measured by cellular organelle staining as a marker of autophagy, an inducible mechanism of cytotoxicity [33][34][35].…”

Section: Discussionmentioning

confidence: 99%

Comparative Investigation of Cellular Effects of Polyethylene Glycol (PEG) Derivatives

et al. 2022

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Nowadays, polyethylene glycols referred to as PEGs are widely used in cosmetics, consumer care products, and the pharmaceutical industry. Their advantageous properties such as chemical stability, low immunogenicity, and high tolerability explain why PEGs are applied in many fields of pharmaceutical formulations including parenteral, topical, ophthalmic, oral, and rectal preparations and also in modern drug delivery systems. Given their extensive use, they are considered a well-known group of chemicals. However, the number of large-scale comparative studies involving multiple PEGs of wide molecular weight range is low, as in most cases biological effects are estimated upon molecular weight. The aim of this publication was to study the action of PEGs on Caco-2 cells and G. mellonella larvae and to calculate the correlation of these effects with molecular weight and osmolality. Eleven PEGs of different molecular weight were used in our experiments: PEG 200, PEG 300, PEG 400, PEG 600, PEG 1000, PEG 1500, PEG 4000, PEG 8000, PEG 10,000, 12,000, and PEG 20,000. The investigated cellular effects included cytotoxicity (MTT and Neutral Red assays, flow cytometry with propidium iodide and annexin V) and autophagy. The osmolality of different molecular weight PEGs with various concentrations was measured by a vapor pressure osmometer OSMOMAT 070 and G. mellonella larvae were injected with the solutions of PEGs. Sorbitol was used as controls of the same osmolality. Statistical correlation was calculated to describe the average molecular weight dependence of the different measured effects. Osmolality, the cytotoxicity assays, flow cytometry data, and larvae mortality had significant correlation with the structure of the PEGs, while autophagosome formation and the proportion of early apoptotic cells showed no statistical correlation. Overall, it must be noted that PEGs must be tested individually for biological effects as not all effects can be estimated by the average molecular weight.

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Section: Discussionmentioning

confidence: 99%

Comparative Investigation of Cellular Effects of Polyethylene Glycol (PEG) Derivatives

et al. 2022

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“…Heat-treated cells, crude cell walls and intracellular extracts from Lactobacillus casei 01 can significantly reduce the activity of HT-29 ( p < 0.05) and play an anti-proliferation role [ 40 ]. A study showed that cell wall protein fractions from Lactobacillus paracasei can inhibit the proliferation of the human colon cancer cell line Caco-2 and promote apoptosis [ 41 ]. Heat-inactivated probiotics can promote the production of Th1-related cytokines and reduce Th2-related cytokines to participate in regulating the immune system [ 42 ].…”

Section: Resultsmentioning

confidence: 99%

Effects of Viable and Heat-Inactivated Bifidobacterium longum D42 on Proliferation and Apoptosis of HT-29 Human Colon Cancer Cells

Zhang,

Cao,

Wang

et al. 2024

Foods

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Bifidobacterium longum is a common probiotic; both viable and heat-inactivated Bifidobacterium longum have many probiotic effects, such as anticancer effects. But some mechanisms of anticancer effects are still unclear, especially for heat-inactivated probiotics. In this study, we analyzed the effects of viable and heat-inactivated Bifidobacterium longum D42 on human colon cancer cells (HT-29). Cell proliferation, membrane permeability and apoptosis were detected by using the CCK-8 method, LDH method and Annexin V-FITC/PI kits. The ROS level and mitochondrial membrane potential were examined using the fluorescent probes DCFH-DA and JC-1. Real-time fluorescence quantitative PCR (RT-qPCR) and Western blot were used to detect the expression of mitochondrial apoptosis pathway genes and proteins. The results showed that viable and heat-inactivated Bifidobacterium longum D42 at concentrations of 1 × 106 CFU/mL significantly inhibited the proliferation of and increased the level of LDH release of HT-29 colon cancer cells. We found that they could increase the apoptosis rate of HT-29 cells. Moreover, they could also induce apoptosis by inducing cells to produce ROS and destroying the mitochondrial membrane potential of cells. Further studies found that they could increase the mRNA transcription and protein expression levels of the Caspase-3, Caspase-9 and Bax genes in cells, and reduce the mRNA transcription and protein expression levels of the Bcl-2 gene. In summary, our findings revealed that viable and heat-inactivated Bifidobacterium longum D42 have inhibitory effects on proliferation and promote the apoptosis of human colon cancer cells, and also have certain adjuvant drug therapeutic effects and have potential application value in the adjuvant treatment of colon cancer.

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“… [ 117 ] Cell wall protein fractions- Lactobacillus paracasei Caco-2 • Induction of apoptosis. [ 118 ] Akt a serine/threonine protein kinase, BAD BCL2-associated agonist of cell death, Bax B-cell lymphoma 2-associated X protein, Bcl-2 B-cell lymphoma 2, Bcl-xl B-cell lymphoma-extra-large, PARP poly ADP ribose polymerase, PI3K phosphatidylinositol 3-kinase, QR quinone reductase, t-Bid truncated BH3 interacting domain death agonist. Caco-2 , HT-29 , SW480 human colorectal adenocarcinoma cell lines …”

Section: Postbiotics and Crcmentioning

confidence: 99%

The Crucial Roles of Diet, Microbiota, and Postbiotics in Colorectal Cancer

Kuru-Yaşar,

Üstün-Aytekin

2024

Curr Nutr Rep

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Purpose of Review Colorectal cancer is the second deadliest cancer in the world, and its prevalence has been increasing alarmingly in recent years. After researchers discovered the existence of dysbiosis in colorectal cancer, they considered the use of probiotics in the treatment of colorectal cancer. However, for various reasons, including the low safety profile of probiotics in susceptible and immunocompromised patient5s, and the risk of developing antibiotic resistance, researchers have shifted their focus to non-living cells, their components, and metabolites. This review aims to comprehensively evaluate the literature on the effects of diet, microbiota, and postbiotics on colorectal cancer and the future of postbiotics. Recent Findings The link between diet, gut microbiota, and colorectal cancer has been established primarily as a relationship rather than a cause-effect relationship. The gut microbiota can convert gastrointestinal tract and dietary factors into either onco-metabolites or tumor suppressor metabolites. There is serious dysbiosis in the microbiota in colorectal cancer. Postbiotics appear to be promising agents in the prevention and treatment of colorectal cancer. Summary It has been shown that various postbiotics can selectively induce apoptosis in CRC, inhibit cell proliferation, growth, invasion, and migration, modulate the immune system, suppress carcinogenic signaling pathways, maintain intestinal epithelial integrity, and have a synergistic effect with chemotherapy drugs. However, it is also reported that some postbiotics are ineffective and may be risky in terms of safety profile in some patients. Many issues need to be researched about postbiotics. Large-scale, randomized, double-blind clinical studies are needed.

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Potential anticancer effects of cell wall protein fractions from Lactobacillus paracasei on human intestinal Caco‐2 cell line

Cited by 21 publications

References 31 publications

Comparative Investigation of Cellular Effects of Polyethylene Glycol (PEG) Derivatives

Comparative Investigation of Cellular Effects of Polyethylene Glycol (PEG) Derivatives

Effects of Viable and Heat-Inactivated Bifidobacterium longum D42 on Proliferation and Apoptosis of HT-29 Human Colon Cancer Cells

The Crucial Roles of Diet, Microbiota, and Postbiotics in Colorectal Cancer

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