Cytotoxicity of partial‐stabilized cement

Wang, Wen-Hsi; Lin, Fa‐Hsuan; Lee, Yuan-Ling; Lin, Chun‐Pin

doi:10.1002/jbm.a.30919

Cited by 9 publications

(5 citation statements)

References 58 publications

(81 reference statements)

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“…Furthermore, adding a drug directly into the sealer would likely result in drug elution and depletion following burst release, which is not an intended use of the NDGP platform. Subsequent iterations of NDGP may potentially explore the use of emerging antibacterial agents. − …”

Section: Resultsmentioning

confidence: 99%

Nanodiamond–Gutta Percha Composite Biomaterials for Root Canal Therapy

Lee¹,

Kim²,

Limansubroto³

et al. 2015

ACS Nano

136

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Root canal therapy (RCT) represents a standard of treatment that addresses infected pulp tissue in teeth and protects against future infection. RCT involves removing dental pulp comprising blood vessels and nerve tissue, decontaminating residually infected tissue through biomechanical instrumentation, and root canal obturation using a filler material to replace the space that was previously composed of dental pulp. Gutta percha (GP) is typically used as the filler material, as it is malleable, inert, and biocompatible. While filling the root canal space with GP is the standard of care for endodontic therapies, it has exhibited limitations including leakage, root canal reinfection, and poor mechanical properties. To address these challenges, clinicians have explored the use of alternative root filling materials other than GP. Among the classes of materials that are being explored as novel endodontic therapy platforms, nanodiamonds (NDs) may offer unique advantages due to their favorable properties, particularly for dental applications. These include versatile faceted surface chemistry, biocompatibility, and their role in improving mechanical properties, among others. This study developed a ND-embedded GP (NDGP) that was functionalized with amoxicillin, a broad-spectrum antibiotic commonly used for endodontic infection. Comprehensive materials characterization confirmed improved mechanical properties of NDGP over unmodified GP. In addition, digital radiography and microcomputed tomography imaging demonstrated that obturation of root canals with NDGP could be achieved using clinically relevant techniques. Furthermore, bacterial growth inhibition assays confirmed drug functionality of NDGP functionalized with amoxicillin. This study demonstrates a promising path toward NDGP implementation in future endodontic therapy for improved treatment outcomes.

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Section: Resultsmentioning

confidence: 99%

Nanodiamond–Gutta Percha Composite Biomaterials for Root Canal Therapy

Lee¹,

Kim²,

Limansubroto³

et al. 2015

ACS Nano

136

View full text Add to dashboard Cite

show abstract

“…Extract testing proved the absence of any toxicity for all the TCs. In this study the extracts were not diluted with culture medium (28,37) so that any potential leachable on cells was not lessened. Cell number on solid cements revealed a good growth and cellular density on all the experimental cements and MTA.…”

Section: Discussionmentioning

confidence: 99%

New Portland Cement–based Materials for Endodontics Mixed with Articaine Solution: A Study of Cellular Response

Gandolfi¹,

Perut²,

Ciapetti³

et al. 2008

Journal of Endodontics

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“…Hence, 1 mL of complete D‐MEM was added in plate wells onto each material. After 24 h at 37°C, the supernatants were separately collected, filtered on 0.22‐μm millipore filters and tested as material extracts 32–34. D‐MEM stored in a polystyrene flask for cell culture and treated as above served as a control for the material extracts.…”

Section: Methodsmentioning

confidence: 99%

Innovative silicate‐based cements for endodontics: A study of osteoblast‐like cell response

Gandolfi

Pagani

Perut

et al. 2008

J Biomedical Materials Res

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Silicate-based filling materials were designed to obtain new endodontic sealers and root-end filling materials with adequate workability and consistency. Four different formulations (TC, TC 1%, TCf 1%, and TCf) were prepared incorporating calcium chloride as accelerant agent. A plasticizing compound (phyllosilicate) was added to TC 1% and TCf 1%. TC and TC 1% were prepared with water, whereas TCf and TCf 1% were mixed with a latex polymer as fluidizing agent. The aim of this study was to assess the in vitro biological compatibility of designed materials. White-MTA and AH Plus were tested as reference materials. Human osteoblast-like Saos-2 cells were challenged in short-term cultures (72 h) with solid materials and with material extracts in culture medium, and cell viability and number, cellular adhesion, and morphology were assessed. The new cements exerted no acute toxicity in the assay systems. Saos-2 like cells adhered and proliferated on solid samples of the experimental cements and MTA whilst AH Plus did not allowed cell growth. The extracts from the latex-containing cements showed some toxicity. By SEM analysis, osteoblast-like cells appeared adherent and spread on the new materials, and showed the maintenance of polygonal osteoblastic phenotype. Similar morphology was observed for cells on MTA, whereas only few cells were noted on the AH Plus surface. In conclusion, the new materials proved non toxic and supported the growth of bone-like cells, and resulted suitable to be used as endodontic sealers and root-end filling materials.

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Cytotoxicity of partial‐stabilized cement

Cited by 9 publications

References 58 publications

Nanodiamond–Gutta Percha Composite Biomaterials for Root Canal Therapy

Nanodiamond–Gutta Percha Composite Biomaterials for Root Canal Therapy

New Portland Cement–based Materials for Endodontics Mixed with Articaine Solution: A Study of Cellular Response

Innovative silicate‐based cements for endodontics: A study of osteoblast‐like cell response

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