Cytotoxicity of PAMAM, PPI and maltose modified PPIdendrimers in Chinese hamster ovary (CHO) and human ovarian carcinoma (SKOV3) cells

Janaszewska, Anna; Maczynska, Katarzyna; Matuszko, Gabriela; Appelhans, Dietmar; Voit, Brigitte; Klajnert, Barbara; Bryszewska, Maria

doi:10.1039/c1nj20489k

Cited by 63 publications

(63 citation statements)

References 45 publications

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“…The toxicity and transfection efficiency are dependent on the generation of PAMAM dendrimer. 23,24 Higher generation dendrimers (>3.0G) show better transfection efficiency but induce higher toxicity whereas, low generation dendrimers (<3.0G) show lower transfection efficiency with higher cell viability. 25,26 Thus, GO modied with low generation PAMAM can yield a carrier with good transfection efficiency with low toxicity.…”

Section: 4mentioning

confidence: 99%

Dendron conjugation to graphene oxide using click chemistry for efficient gene delivery

Madras

Chatterjee

2015

RSC Adv.

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Owing to its large surface area and rapid cellular uptake, graphene oxide (GO) is emerging as an attractive candidate material for delivery of drugs and genes. The inherent sp 2 p-p interaction of GO helps to carry drugs and single stranded RNA (ssRNA) but there is no such interaction with double stranded DNA (dsDNA).In this work, a polyamidoamine (PAMAM) dendron was conjugated with nano GO (nGO) through "click" chemistry to improve the DNA complexation capability of GO as well as its transfection efficiency. The DNA complexation capability of GO was significantly enhanced after dendronization of GO yielding spherical nanosized (250-350 nm) particles of the dendronized GO (DGO)/pDNA complex with a positive zeta potential. The transfection efficiency of GO dramatically increased after conjugation of the PAMAM dendron. Transfection efficiency of 51% in HeLa cells with cell viability of 80% was observed. The transfection efficiency was significantly higher than that of polyethyleneimine 25 kDa (27% efficiency) and also surpassed that of lipofectamine 2000 (47% efficiency). The uptake of the DGO/pDNA complex by the caveolae mediated endocytosis pathway may significantly contribute to the high transfection efficiency. Thus, dendronized GO is shown to be an efficient gene carrier with minimal toxicity and is a promising candidate for use as a nonviral carrier for gene therapy.

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Section: 4mentioning

confidence: 99%

Dendron conjugation to graphene oxide using click chemistry for efficient gene delivery

Madras

Chatterjee

2015

RSC Adv.

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“…These dendrimers have also been shown to encapsulate small guest molecules, making them suitable for a range of biomedical applications [59]. The responses of many cell types to PAMAM dendrimers have been evaluated using a variety of toxicity assays that investigate cell death, metabolism, enzyme leakage and DNA damage [11,[60][61][62][63]. Examples of recent cytotoxicity studies are summarized below in Table 2.…”

Section: Cytotoxicity Of Pamam Dendrimersmentioning

confidence: 99%

PAMAM dendrimer - cell membrane interactions

Fox

Richardson

Briscoe

2018

Advances in Colloid and Interface Science

169

125

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General rightsThis document is made available in accordance with publisher policies. Please cite only the published version using the reference above. 2018 PAMAM dendrimers have been conjectured for a wide range of biomedical applications due to their tuneable physicochemical properties. However, their application has been hindered by uncertainties in their cytotoxicity, which is influenced by dendrimer generation (i.e. size and surface group density), surface chemistry, and dosage, as well as cell specificity. In this review, biomedical applications of polyamidoamine (PAMAM) dendrimers and some related cytotoxicity studies are first outlined. Alongside these in vitro experiments, lipid membranes such as supported lipid bilayers (SLBs), liposomes, and Langmuir monolayers have been used as cell membrane models to study PAMAM dendrimer-membrane interactions. Related experimental and theoretical studies are summarized, and the physical insights from these studies are discussed to shed light on the fundamental understanding of PAMAM dendrimer-cell membrane interactions. We conclude with a summary of some questions that call for further investigations.a b s t r a c t a r t i c l e i n f o Available online 27 June

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“…However, this toxicity is mainly related to the positive charge present on the surface of the so-called cationic dendrimers. [6][7][8] Appropriate modifications of the dendrimers' surface can greatly reduce their toxicity. 9 Such modified dendrimers can be used in nanomedical applications, as they possess the advantage of dendrimers' spatial structure and simultaneously possess reduced toxic activity.…”

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confidence: 99%