Cytotoxicity of Methanol Extracts from Cudrania tricuspidata Bureau

Abstract

: In order to develop as a natural source of anticancer materials of Cudrania tricuspidata, the cytotoxicity of methanol extracts by harvesting parts and times against 8 cell lines including 293 (normal kidney cells) and A-431 (epidermoid carcinoma cells) were investigated using MTT assay. All harvesting parts had hardly cytotoxicity against 293. And methanol extracts of stem bark and root bark showed very high cytotoxicities against 7 cancer cell lines. The cytotoxicity was the highest against HeLa (cervix adenocarcinoma cells) and followed by MCF-7 (breast adenocarcinoma cells), AGS (stomach adenocarcinoma cells), HT-29 (colon adenocarcinoma cells), HepG2 (hepatoblastoma cells), A549 (lung carcinoma cells) and A-431. By the way, leaf extract had a cytotoxicity against only AGS and ripe fruit extract had no cytotoxicity. Among harvesting times, the cytotoxicity of root bark were high from April to September but that of stem bark showed a little difference. These results showed that anticancer activities of Cudrania tricuspidata extracts were eventful changes by harvesting parts and times.

Effect of Fermented Cudrania tricuspidata Fruit Extracts on the Generation of the Cytokines in Mouse Spleen Cells

“…꾸지뽕(Cudrania tricuspidata)는 뽕나무과에 속하는 낙엽교 목으로서 한국을 비롯한 일본, 중국과 러시아 동부지역 등에 서 많이 자생하며, 서아시아지역에서는 전통약초로서 사용되 어 온 약재 중 하나로서 줄기가 주로 사용되며, 잎과 열매는 식용작물으로 이용되어 왔다 [5,10]. 전통적으로 약리활성이 알려진 꾸지뽕의 줄기는 xanthones, flavonoids 등의 폴리페놀 (polyphenol) 성분이 다량 함유되어 있으며, tyrosinase 저해, 항산화, 세포독성, 항염증 활성에 대한 연구가 보고되어 있다 [12,13,17,22,24,30].…”