Cytotoxicity of Local Anesthetics on Human Mesenchymal Stem Cells in Vitro

Breu, Anita; Eckl, S.; Zink, Wolfgang; Kujat, Richard; Angele, Peter

doi:10.1016/j.arthro.2013.06.018

Cited by 72 publications

(69 citation statements)

References 44 publications

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“…Local anesthetics are not only used for nerve blocks, pain control, and anti-arrhythmia and anti-inflammatory purposes, but can also cause neuronal cell death and the death of other cell types (18)(19)(20). It has even been speculated that the cytotoxicity of local anesthetics could play an important antitumor role.…”

Section: Discussionmentioning

confidence: 99%

Lidocaine inhibits the invasion and migration of TRPV6-expressing cancer cells by TRPV6 downregulation

et al. 2016

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Abstract.It is well known that local anesthetics have a broad spectrum of pharmacological actions, acting as nerve blocks, and treating pain and cardiac arrhythmias via blocking of the sodium channel. The use of local anesthetics could reduce the possibility of cancer metastasis and recurrence following surgical tumor excision. The purpose of the present study was to investigate the inhibitory effect of lidocaine upon the invasion and migration of transient receptor potential cation channel subfamily V member 6 (TRPV6)-expressing cancer cells. Human breast cancer MDA-MB-231 cells, prostatic cancer PC-3 cells and ovarian cancer ES-2 cells were treated with lidocaine. Cell viability was quantitatively determined by MTT assay. The migration of the cells was evaluated using the wound healing assay, and the invasion of the cells was assessed using a Transwell assay. Calcium (Ca 2+ ) measurements were performed using a Fluo-3 AM fluorescence kit. The expression of TRPV6 mRNA and protein in the cells was determined by quantitative-polymerase chain reaction and western blot analysis, respectively. The results suggested that lidocaine inhibits the cell invasion and migration of MDA-MB-231, PC-3 and ES-2 cells at lower than clinical concentrations. The inhibitory effect of lidocaine on TRPV6-expressing cancer cells was associated with a reduced rate of calcium influx, and could occur partly as a result of the downregulation of TRPV6 expression. The use of appropriate local anesthetics may confer potential benefits in clinical practice for the treatment of patients with TRPV6-expressing cancer.

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Section: Discussionmentioning

confidence: 99%

Lidocaine inhibits the invasion and migration of TRPV6-expressing cancer cells by TRPV6 downregulation

et al. 2016

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“…22 Moreover, when MSC monolayers were exposed directly to bupivacaine, ropivacaine, and mepivacaine, all three showed time-and concentration-dependent cytotoxic effects. 23 Based on this in vitro work, it is clear that local anesthetics may have a deleterious effect on the function of MSCs.…”

Section: Potential Implications Of Anesthetics On Mscsmentioning

confidence: 92%

“…22 En outre, lorsque des monocouches de CSM ont été exposées directement à de la bupivacaïne, de la ropivacaïne ou de la mépivacaïne, ces trois agents ont tous démontré des effets cytotoxiques dépendants de la durée et de la concentration. 23 Sur la base de ces travaux in vitro, il est clair que les anesthésiques locaux pourraient avoir un effet délétère sur la fonction des CSM.…”

Section: Les Voies De Transmission Thérapeutiques Des Csmunclassified

Adult stem cells: potential implications for perioperative medicine

Lalu

Barron

Stewart

et al. 2014

Can J Anesth/J Can Anesth

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“…It was reported that the effect of lidocain and prilocaine as primary buccal infiltration was similar to each other when compared with articaine (8,10). Local anesthetics including lidocaine applied topically have been reported to use for surgical anaesthesia with reduced cardiotoxicity and central nervous system toxicity (11,12). However, many studies have shown their cytotoxicity towards several cultured cells (13,14).…”

Section: Discussionmentioning

confidence: 99%