Lidocaine inhibits the invasion and migration of TRPV6-expressing cancer cells by TRPV6 downregulation

Abstract

Abstract.It is well known that local anesthetics have a broad spectrum of pharmacological actions, acting as nerve blocks, and treating pain and cardiac arrhythmias via blocking of the sodium channel. The use of local anesthetics could reduce the possibility of cancer metastasis and recurrence following surgical tumor excision. The purpose of the present study was to investigate the inhibitory effect of lidocaine upon the invasion and migration of transient receptor potential cation channel subfamily V member 6 (TRPV6)-expressing cancer cells. Human breast cancer MDA-MB-231 cells, prostatic cancer PC-3 cells and ovarian cancer ES-2 cells were treated with lidocaine. Cell viability was quantitatively determined by MTT assay. The migration of the cells was evaluated using the wound healing assay, and the invasion of the cells was assessed using a Transwell assay. Calcium (Ca 2+ ) measurements were performed using a Fluo-3 AM fluorescence kit. The expression of TRPV6 mRNA and protein in the cells was determined by quantitative-polymerase chain reaction and western blot analysis, respectively. The results suggested that lidocaine inhibits the cell invasion and migration of MDA-MB-231, PC-3 and ES-2 cells at lower than clinical concentrations. The inhibitory effect of lidocaine on TRPV6-expressing cancer cells was associated with a reduced rate of calcium influx, and could occur partly as a result of the downregulation of TRPV6 expression. The use of appropriate local anesthetics may confer potential benefits in clinical practice for the treatment of patients with TRPV6-expressing cancer.

Lidocaine inhibits proliferation and metastasis of lung cancer cell via regulation of miR-539/EGFR axis

“…On the one hand, lidocaine was capable of repressing tumour cells growth, like colon adenocarcinoma cells [10] and gastric cancer cells [11]. On the other hand, lidocaine could inhibit tumour metastasis by controlling the capacities of migration and invasion [11,23]. Herein, the anti-growth and anti-metastatic effects of lidocaine towards A549 and NCI-H1299 cells were illustrated, indicating the anti-tumour properties of lidocaine on lung cancer.…”

Anti-tumor effects of lidocaine on human gastric cancer cells in vitro

“…In addition, according to reports, lidocaine has many functions, including depressing the infl ammatory reaction (17,18), protecting against an acute lung injury (19)(20)(21) and reducing postoperative cognitive dysfunction (POCD) (22)(23)(24). There is also the study, which indicated that lidocaine might have anti-cancer actions in a wide range of cancer cells (25)(26)(27)(28)(29), and it may have sensitizing effects on anti-cancer drugs (30,31). Up to date, no reports have investigated whether lidocaine can affect the viability, invasion and migration of gastric cancer cells, and its potential anti-cancer effects on gastric cancer cells were not fully understood.…”

Antitumor effects of Tv1 venom peptide in liver cancer

“…46,47 TRPV6 mRNA and protein expression has been detected in ovarian cancer and other cancer types, such as breast, prostate, thyroid and colon cancer. 35,48 . Due to the involvement of TRPV6 channels in cancer cell proliferation 46,49 and its overexpression in numerous cancer models, attempts have been made to virtually screen TRPV6's Ca 2+ channel inhibitors 50 .…”