Cytotoxicity of latex and pharmacobotanical study of leaves and stem of Euphorbia umbellata (Janaúba)

Luz, Lívia Eidam Camargo; Paludo, Kátia Sabrina; Santos, Vera Lúcia Pereira dos; Franco, Célia Regina Cavichiolo; Klein, Traudi; Silva, Rozi Z.; Beltrame, Flávio Luís; Budel, Jane Manfron

doi:10.1016/j.bjp.2015.07.005

Cited by 22 publications

(20 citation statements)

References 28 publications

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“…Morpho-anatomical data are used to mitigate this problem. This technique uses morphological and anatomical features to characterize and differentiate similar species (Luz et al, 2015;Wosch et al, 2015;Porto et al, 2016), especially when the botanicals are marketed in a fragmented or powdered form (American Herbal Pharmacopeia, 2011).…”

Section: Introductionmentioning

confidence: 99%

Comparative morphoanatomical analysis of Mikania species

Almeida

Hirt

Raeski

et al. 2017

Revista Brasileira de Farmacognosia

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Please cite this article in press as: de Almeida, V.P., et al. Comparative morphoanatomical analysis of Mikania species. Revista Brasileira de Farmacognosia (2016), http://dx. a b s t r a c tMikania belongs to the Asteraceae family and includes a wide range of promising pharmacological activities. Several species of Mikania, which is popularly known in Brazil as "guaco", occur in Southern Brazil and their external morphology is similar. The aim of this study was to investigate the morpho-anatomical characteristics of the leaf and stem of Mikania campanulata, Mikania cordifolia, Mikania glomerata, Mikania hastato-cordata, Mikania microptera and Mikania sessilifolia as a means of providing additional support for differentiating these taxa. The leaves and stems were investigated by employing scanning electron microscopy and light microscopy techniques. The morphological features of Mikania spp. leaves make it possible to differentiate between the species; nevertheless, when the plants were fragmented or pulverized the anatomical features of the leaves and stems supplied additional helpful data in this regard. The main anatomical characteristics were presence of hypodermis and lens shaped epidermal cells, set of trichomes; midrib, petiole and stem shape and vascular pattern; sclerenchymatous ring in the cortex, sclerenchymatous cells and secretory ducts in the pith.

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Section: Introductionmentioning

confidence: 99%

Comparative morphoanatomical analysis of Mikania species

Almeida

Hirt

Raeski

et al. 2017

Revista Brasileira de Farmacognosia

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“…S. grantii is a species of the Euphorbiaceae family, also known as "milagrosa" or "janauba, " has been a scientific subject for the anticancer drug treatment since different compounds can be found in its plant material. Scientific evidence has found the anticancer activity of steroids, terpenes (diterpenes, sesquiterpenes, and triterpenes), phenolic compounds, and proteins from S. grantii (25,27,34,35). Taxon evidence shows that Euphorbia umbellata (Pax) Bruyns and Synadenium umbellatum are scientific synonyms, as well as their role in anticancer drug development (27).…”

Section: Discussionmentioning

confidence: 99%

“…Portion of the sample was used in the physicochemical characterization test and in the identification of active compounds. For in vitro and in vivo studies, 1 g of latex was dissolved in distilled water into a 10 ml final stock solution according to Luz et al (27). Then, a decrease in concentrations was made, and new stock solutions with concentrations of 0.75, 1, 1.5, 3, and 6 mg/ml were originated.…”

Section: Plant Materialsmentioning

confidence: 99%

Effects of the Latex of Synadenium grantii Hook F. (Euphorbiaceae) on a Preclinical Model of Canine Prostate Cancer

et al. 2021

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Prostatic cancer (PC) stands out in terms of its occurrence, pathophysiology, and unfavorable prognostics in humans and dogs. Natural drugs bear an integrative potential for conventional antineoplastic treatments. In this context, the bioproducts of Synadenium grantii have been empirically used in different parts of Brazil for the integrative treatment of prostate cancer in humans. However, there is no availability of scientific evidence of the antitumor effects of S. grantii. Therefore, this study aimed to investigate the bioactive compounds in the latex of S. grantii using the high-resolution mass spectrophotometry (HRMS) and to evaluate its cytotoxic effects on primary canine PC cell cultures. Four fragments of phorbol ester were identified as potential bioactive compounds using the HRMS. With the help of an MTT ([3-(4,5-dimethyldiazol-2-yl)-2,5 diphenyltetrazolium bromide]) assay, two canine prostatic carcinoma cell lines (PC 1 and PC2) showed a decrease in the tumor cell count, with an Inhibitory concentration 50 (IC50)of 0.8469 and 0.6068 mg/ml, respectively, for PC1 and PC2. In conclusion, the latex of S. grantii contains phorbol esters in its composition, and its aqueous solution has a cytotoxic effect on canine metastatic PC cells in vitro.

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“…The cytotoxic potential of the latex from E. umbellata has been described previously for B16F10 (melanoma), HRT-18 (colon cancer) and HeLa (cervical adenocarcinoma) cell lineages; [11,13] latex extracts have been evaluated against HRT-18, HeLa and Jurkat (leukemia) lineages, with the hexane extract presenting higher cytotoxicity than the chloroform, ethyl acetate and methanol extracts. [14] Chemical studies suggested the presence of several compounds that could be related with the cytotoxic activity of E. umbellata; therefore, a preliminary cytotoxic evaluation was developed with Hex and its fractions.…”

Section: Cytotoxic Assaymentioning

confidence: 99%

Enriched Terpenes Fractions of the Latex of Euphorbia umbellata Promote Apoptosis in Leukemic Cells

Cruz

Kanunfre

Andrade

et al. 2020

Chemistry & Biodiversity

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The current study was carried out by a bioguided fractionation of a hexane extract of the latex of Euphorbia umbellata against leukemic cells. Samples were analyzed by NMR, GC/MS, triterpenes quantification, and MTT reduction assay. Morphological, cell cycle, mitochondrial membrane potential and caspases 3/7 analyses were performed for the dichloromethane and ethanol fractions, and selectivity index for the dichloromethane fraction. NMR analysis presented characteristic signals of terpenes and steroids, data were confirmed by the quantification of triterpenes and GC/MS analysis. MTT reduction assay demonstrated that HL‐60 was the most sensitive cell lineage against dichloromethane and ethanol fractions. Compounds of these matrices caused morphological changes compatible with apoptosis induction, altered cell cycle, increment of depolarized population cells and activation of caspases 3/7. Selectivity indices were higher than 22.44. Bioguided‐fractionation study showed that samples of the latex of E. umbellata raised the activity of the phytocomplex against leukemic cells, and the cytotoxicity can be associated with an apoptosis pathway.

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Cytotoxicity of latex and pharmacobotanical study of leaves and stem of Euphorbia umbellata (Janaúba)

Cited by 22 publications

References 28 publications

Comparative morphoanatomical analysis of Mikania species

Comparative morphoanatomical analysis of Mikania species

Effects of the Latex of Synadenium grantii Hook F. (Euphorbiaceae) on a Preclinical Model of Canine Prostate Cancer

Enriched Terpenes Fractions of the Latex of Euphorbia umbellata Promote Apoptosis in Leukemic Cells

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