Cytotoxicity of hard and soft denture lining materials

Abstract

The cytotoxicity of nine soft and hard lining materials (Mollosil Plus, Ufi Gel SC, Visco-gel, Molloplast-B, GC Tissue Conditioner, Vertex Rapid Simplified, GC Reline Hard, Vertex Self-Curing, Ufi Gel hard C) was evaluated using human gingival fibroblasts (HGFs). Twelve disk samples per lining material were prepared and incubated for 24, 48, 72, and 96 h. Cytotoxicity of each lining material's extract on cultured HGFs was measured using XTT assay. Data were analyzed using one-way ANOVA, post hoc Dunnett's T3 and Bonferroni tests at a significance level of p<0.05. At all incubation periods, all the hard lining materials (Vertex-SC, GC Reline Hard, Vertex-RS, and Ufi Gel hard C) showed cell viability higher than 90%. Among the soft lining materials, although there were no significant differences in cell viability among the different incubation periods for each lining material (p>0.05), autopolymerized acrylic-based GC Tissue Conditioner showed significantly lower cell viability than the other soft lining materials at each incubation period. Among the hard lining materials, there were no significant differences both among the materials and across all incubation periods for each lining material (p>0.05). In conclusion, all soft and hard liners exhibited good biocompatibility regardless of incubation time, except for GC Tissue Conditioner.

Analysis of radiopacity, pH and cytotoxicity of a new bioceramic material

“…Although the MTT assay measures the mitochondrial metabolic activity rate as an estimate of cell viability, an observed excess of metabolic activity may also represent a response to increased cellular stress due to toxicity, thus caution is recommended with the use of MTT as its estimation of cell viability may be misleading 28 , 29 . Moreover, the MTT requires the cells to be killed, making it impossible to follow up on the cells in culture 1 , and yet requires an extended incubation time with lower sensitivity than the XTT 3 . Our experimental model designed to obtain extract media for the cell viability assay and the use of human periodontal ligament cells resembles a clinical scenario, simulating the amount and surface area of the material that usually comes in contact with surrounding tissues in most clinical applications 8 , 9 , 12 , 29 .…”

Histopathological Changes by the Use of Soft Reline Materials: A Rat Model Study

“…Since the increase of the thickness of the oral mucosa under the denture base is also considered a physiological response to mechanical loading [47]–[52], and no compressive forces was reproduced in this study because the plates were fixed in the molars, we may not expect any increased proliferative activity of the epithelial cells due the mechanical compression factors [49]–[52]. The histopathological changes assessed may be a response of the epithelium to the release of toxic substances [20]–[21], [26], [41], [52]–[54] and/or residual monomers [24], [31]. Despite the cytotoxicity reported in vitro [26], in this present in vivo study no histopathological changes of the epithelium were observed forlining resins tested without the application of post-polymerization heat treatment.…”

Dental protetik materyallerin biyolojik uyumluluğu ve test yöntemleri

“…En düşük hücre canlılığını gösteren GC Tissue Conditioner dışında diğer tüm sert ve yumuşak astar maddelerinin biyouyumlu olduğu bildirilmiştir. 60 …”