Cytotoxicity of Gemcitabine-Loaded-Microemulsions in Breast and Colon Cancer Cells

Alkhatib, Mayson H.; Alkhayyal, Norah S.

doi:10.4314/tjpr.v13i2.8

Cited by 6 publications

(6 citation statements)

References 20 publications

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“…Blank microemulsions showed a relevant cytotoxic activity (30-50% growth inhibition) against HCT-116 colon cancer cells. Moreover, cells treated with drug-loaded microemulsions showed more intense apoptosis than those exposed to the drug solution [78].…”

Section: Microemulsions For Colorectal Cancer Treatmentsmentioning

confidence: 95%

“…Microemulsions based on 37% Cremophor EL/transcutol mixture 2:1, 29% Capryol 90, and 34% water were loaded with docetaxel and tested in colon cancer cells (HCT-116), [77] Droplets without drug (blank microemulsion) had sizes in the 89±25 nm range, while after loading the size increased up to 205± 13 nm, All microemulsions showed high cytotoxic effects on colon cancer cells compared with the reference Taxotere, but caused less detrimental effects on non-tumoral human epithelial cells. Therefore, docetaxel-loaded microemulsions showed improved therapeutic efficacy and safety [78]. In other work, microemulsions were prepared combining Tween 80, Span 20, ethanol, isopropyl myristate and water and loaded with gemcitabine [78], a chemotherapeutic agent effective against many solid tumors and also employed in colorectal cancer treatment [79].…”

Section: Microemulsions For Colorectal Cancer Treatmentsmentioning

confidence: 99%

“…Therefore, docetaxel-loaded microemulsions showed improved therapeutic efficacy and safety [78]. In other work, microemulsions were prepared combining Tween 80, Span 20, ethanol, isopropyl myristate and water and loaded with gemcitabine [78], a chemotherapeutic agent effective against many solid tumors and also employed in colorectal cancer treatment [79]. Drug-loaded microemulsions had high hemolysis activity (17-21 %) although below that exhibited by a drug solution of similar concentration (37%).…”

Section: Microemulsions For Colorectal Cancer Treatmentsmentioning

confidence: 99%

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Microemulsions for Colorectal Cancer Treatments. General Considerations and Formulation of Methotrexate

Flores¹,

Rial-Hermida²,

Ramírez³

et al. 2016

MRMC

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Microemulsions combine the advantages of emulsions with those of nanocarriers, overcoming the stability problems of the former and providing facile scalable systems with compartments adequate for high drug loadings. Recently, microemulsions are gaining attention in the formulation of anticancer drugs not only for topical treatment, but also for systemic delivery as well as for the development of theranostic systems. The aim of this paper is two-fold. First, an updated review about general features, preparation, characterization and pharmaceutical applications, with a special focus on colorectal cancer, is provided. Second, a case study of formulation of methotrexate in microemulsions is presented. Various essential oils (menthol, trans-anethole, α-tocopherol) and surfactants (TPGS-1000, Maxemul 6112, Noigen RN-20) were investigated for the preparation of o/w microemulsions for the delivery of methotrexate, and the ability of methotrexate-loaded microemulsions to inhibit cancer cell growth was then evaluated. Disregarding the surfactants used, menthol and trans-anethole led to cytotoxic microemulsions, whereas α-tocopherol based-formulations induced cell proliferation. These findings highlight the role that the oily component may play in the efficacy and safety of the microemulsions.

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Section: Microemulsions For Colorectal Cancer Treatmentsmentioning

confidence: 95%

Section: Microemulsions For Colorectal Cancer Treatmentsmentioning

confidence: 99%

Section: Microemulsions For Colorectal Cancer Treatmentsmentioning

confidence: 99%

See 1 more Smart Citation

Microemulsions for Colorectal Cancer Treatments. General Considerations and Formulation of Methotrexate

Flores¹,

Rial-Hermida²,

Ramírez³

et al. 2016

MRMC

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“…Nowadays, nano-emulsion and microemulsions are being used in drug delivery systems. Microemulsions loaded with gemcitabine [61] have been used against many solid tumors and are also used in colorectal cancer treatment [62]. The most attractive point regarding drug-loaded microemulsions is their high hemolysis activity (17-21%).…”

Section: Emulsions In Cancer Treatmentmentioning

confidence: 99%

Advances in Polymeric Colloids for Cancer Treatment

Ali

Althakfi²,

Suhail³

et al. 2022

Polymers

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Polymer colloids have remarkable features and are gaining importance in many areas of research including medicinal science. Presently, the innovation of cancer drugs is at the top in the world. Polymer colloids have been used as drug delivery and diagnosis agents in cancer treatment. The polymer colloids may be of different types such as micelles, liposomes, emulsions, cationic carriers, and hydrogels. The current article describes the state-of-the-art polymer colloids for the treatment of cancer. The contents of this article are about the role of polymeric nanomaterials with special emphasis on the different types of colloidal materials and their applications in targeted cancer therapy including cancer diagnoses. In addition, attempts are made to discuss future perspectives. This article will be useful for academics, researchers, and regulatory authorities.

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“…ME improves the cytotoxic effect with respect to the free drug, implying a better effective drug uptake inside the cells. In other words, the anticancer drug accumulation in the cancerous cells would get enhanced besides reducing its side effect on the other cells 14,15,16 . The objective of this in vitro study was to examine the antitumor activity of the combination therapy of GEM and ATV, encapsulated in a ME formulation designed by Tsai et al 17 , in HCT116 human colon cancer cells.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic Effect of the Combination of Gemcitabine and Atorvastatin Loaded in Microemulsion on the HCT116 Colon Cancer Cells

Alkhatib

Al-Saedi

Backer

2017

IJPCR

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The combination of anticancer drugs in nanoparticles has great potential as a promising strategy to maximize efficacies by eradicating resistant, reduce the dosage of the drug and minimize toxicities on the normal cells. Gemcitabine (GEM), a nucleoside analogue, and atorvastatin (ATV), a cholesterol lowering agent, have shown anticancer effect with some limitations. The objective of this in vitro study was to evaluate the antitumor activity of the combination therapy of GEM and ATVencapsulated in a microemulsion (ME) formulation in the HCT116 colon cancer cells. The cytotoxicity and efficacy of the formulation were assessed by the 3- (4,5dimethylthiazole-2-yl)-2,5-diphyneltetrazolium bromide (MTT) assay. The mechanism of cell death was examined by observing the morphological changes of treated cells under light microscope, identifying apoptosis by using the ApopNexin apoptosis detection kit, and viewing the morphological changes in the chromatin structure stained with 4′,6-diamidino-2-phenylindole (DAPI) under the inverted fluorescence microscope. It has been found that reducing the concentration of GEM loaded on ME (GEM-ME) from 5μM to 1.67μM by combining it with 3.33μM of ATV in a ME formulation (GEM/2ATV-ME) has preserved the strong cytotoxicity of GEM-ME against HCT116 cells. The current study proved that formulating GEM with ATV in ME has improved the therapeutic potential of GEM and ATV as anticancer drugs.

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Cytotoxicity of Gemcitabine-Loaded-Microemulsions in Breast and Colon Cancer Cells

Abstract: Purpose: To evaluate the antitumor activity of gemcitabine (GEM), incorporated in microemulsions with varying surfactant-to-oil (S/O) ratio, against MCF-7 breast cancer cells and HCT . Conclusion: Microemulsion formulations improved the efficacy of GEM and induced apoptosis in

Cited by 6 publications

References 20 publications

Microemulsions for Colorectal Cancer Treatments. General Considerations and Formulation of Methotrexate

Microemulsions for Colorectal Cancer Treatments. General Considerations and Formulation of Methotrexate

Advances in Polymeric Colloids for Cancer Treatment

Cytotoxic Effect of the Combination of Gemcitabine and Atorvastatin Loaded in Microemulsion on the HCT116 Colon Cancer Cells

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