Cytotoxicity of Ficus Crocata Extract on Cervical Cancer Cells and Protective Effect against Hydrogen Peroxide-Induced Oxidative Stress in HaCaT Non-Tumor Cells

Abstract

Oxidative stress causes several chronic diseases including cancer. Some chemotherapeutic agents are not selective against tumor cells, causing oxidative stress in non-tumor cells. This study aimed to evaluate the cytotoxic effect of acetone extract of Ficus crocata(Miq.) Mart. ex Miq. (F. crocata) leaves (Ace-EFc) on cervical cancer cells, as well as its protective effect on hydrogen peroxide (H2O2)-induced lipoperoxidation and cytotoxicity in non-tumor HaCaT cells. Antioxidant activity was determined using the DPPH and ABTS radicals. Cell viability and lipoperoxidation were determined with MTT and 1-methyl-2-phenylindole assays, respectively. A model of H2O2-induced cytotoxicity and oxidative damage in HaCaT cells was established. HaCaT cells were exposed to the extract before or after exposure to H2O2, and oxidative damage and cell viability were evaluated. Ace-EFc inhibited the DPPH and ABTS radicals and showed a cytotoxic effect on SiHa and HeLa cells. Furthermore, the extract treatment had a protective effect on hydrogen peroxide-induced lipoperoxidation and cytotoxicity, avoiding the increase in MalonDiAldehyde (MDA) levels and the decrease in cell viability (p < 0.001). These results suggest that the metabolites of F. crocata leaves possess antioxidant and cytoprotective activity against oxidative damage. Thus, they could be useful for protecting cells from conditions that cause oxidative stress.

In situ Forming Hyperbranched PEG—Thiolated Hyaluronic Acid Hydrogels With Honey-Mimetic Antibacterial Properties

“…Two internationally well-known fibroblast (L929) and epithelial (HaCaT) cell lines were used for the in vitro analysis, following DIN EN ISO 10993-5 standards. Furthermore, they represent typical starting cell lines for future investigations in the field of external wound treatment ( Rajan et al, 2020 ; Ribeiro et al, 2020 ; De la Cruz-Concepción et al, 2021 ).…”

Ficus spp.: Phytochemical composition and medicinal potential

“…Hot water is a good solvent strategy, as it has improved the extraction of antioxidant compounds in roots from F. dubia (Suttisansanee et al, 2021). The acetone extract from the leaves of F. crocata showed antioxidant activity (IC50, 107.05 ± 2.61 µg/mL by DPPH˙ and 1.47 ± 1.21 µg/mL by ABTS+˙) higher than ascorbic acid (IC50, 118.82 ± 2.48 µg/mL by DPPH˙ and 2.22 ± 0.80 µg/mL by ABTS+˙) (Cruz-Concepción et al, 2021).…”

“…The presence of terpenes was also identified, among them the lupeol triterpenes (Cruz-Concepción et al, 2021;El-Beltagi et al, 2019;Knothe et al, 2019;Mbougnia et al, 2021;Sánchez-Valdeolívar et al, 2020), squalene (Cruz-Concepción et al, 2021;Knothe et al, 2019;Sánchez-Valdeolívar et al, 2020;Tian et al, 2020), lanosterol (El-Beltagi et al, 2019), diterpene phytol (Cruz-Concepción et al, 2021;El-Beltagi et al, 2019;Lawal et al, 2016), monoterpene linalool, and the sesquiterpene β-caryophyllene (Lawal et al, 2016;Soltana et al, 2017;Tian et al, 2020), mainly identified in leaves (F.…”

“…The steroid β-sitosterol was identified in different parts of F. carica, F. crocata, F. elastica, F. natalensis, F. racemosa and F. sycomorus (Bopage et al, 2018;Cruz-Concepción et al, 2021;El-Beltagi et al, 2019;Mbougnia et al, 2021;Mustafa et al, 2021;Sánchez-Valdeolívar et al, 2020; J. M. E. Teinkela, Noundou, et al, 2016). The stigmasterol and campesterol phytosterols were identified in the leaves of F. crocata (Sánchez-Valdeolívar et al, 2020), and F. sycomorus (El-Beltagi et al, 2019).…”

“…Organic acids such as oxalic, aconitic, citric, tartaric, malic, quinic and fumaric acids were identified in the aqueous extracts of F. exasperata leaves and bark (Mouho et al, 2018). Vitamin E was identified in the acetone extracts of the F. crocata leaves (α-tocopherol) (Cruz-Concepción et al, 2021;Sánchez-Valdeolívar et al, 2020).…”