Cytotoxicity of Fast-set Conventional and Resin-modified Glass Ionomer Cement Polymerized at Different Times on SHED

Abidin, Roszeyana Mohd Zainal; Luddin, Norhayati; Omar, Norsuhana; Ahmed, Hany Mohamed Aly

doi:10.17796/1053-4628-39.3.235

Cited by 5 publications

(3 citation statements)

References 27 publications

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“…In contrast, Ionolux was cytotoxic. In agreement with other authors [25], we believe that unpolymerized HEMA is responsible for the toxicity, a finding mentioned by Bakapoulou et al [26], who showed an evident cytotoxic effect of HEMA on dental pulp cells, which can severely disturb the odontogenic differentiation potential of HEMA, thus compromising pulp-tissue homeostasis and repair. Other reports have shown that several components such as monomers, photo-initiators, or fillers may be related to the cytotoxicity of glass ionomers [27,28,29,30].…”

Section: Discussionsupporting

confidence: 93%

In Vitro Evaluation of the Biological Effects of ACTIVA Kids BioACTIVE Restorative, Ionolux, and Riva Light Cure on Human Dental Pulp Stem Cells

et al. 2019

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This study aimed to analyze the biological effects of three new bioactive materials on cell survival, migration, morphology, and attachment in vitro. ACTIVA Kids BioACTIVE Restorative (Pulpdent, Watertown, MA, USA) (Activa), Ionolux (Voco, Cuxhaven, Germany), and Riva Light Cure UV (SDI, Bayswater, Australia) (Riva) were handled and conditioned with a serum-free culture medium. Stem cells from human dental pulp (hDPSCs) were exposed to material extracts, and metabolic activity, cell migration, and cell morphology were evaluated. Cell adhesion to the different materials was analyzed by scanning electron microscopy (SEM). The chemical composition of the materials was evaluated by energy-dispersive X-ray (EDX). One-way analysis of variance followed by a Tukey test was performed (p < 0.05). Ionolux promoted a drastic reduction in metabolic activity and wound closure compared to the control (p < 0.05), whereas Activa induced adequate metabolic activity and cell migration. Moreover, SEM and immunofluorescence analysis showed abundant cells exposed to Activa. The materials showed different surface morphologies, and EDX spectra exhibited different peaks of C, O, Si, S, Ca, and F ions in glass ionomer cements. The results showed that Activa induced cell migration, cell attachment, and cell viability to a greater extent than Riva and Ionolux.

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Section: Discussionsupporting

confidence: 93%

In Vitro Evaluation of the Biological Effects of ACTIVA Kids BioACTIVE Restorative, Ionolux, and Riva Light Cure on Human Dental Pulp Stem Cells

et al. 2019

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“…GICs are recognized for their wide range of applications in dentistry based on their excellent properties (2,17,18). Despite these characteristics, the biocompatibility of GICs is not always satisfactory, with several reports describing their cytotoxicity (19)(20)(21)(22).…”

Section: Discussionmentioning

confidence: 99%

Hydroxyapatite decreases cytotoxicity of a glass ionomer cement by calcium fluoride uptake in vitro

Angel‐Mosqueda

Hernandez-Delgadillo

Rodríguez-Luis

et al. 2017

Journal of Applied Biomaterials & Functional Materials

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“…Calibration curves for each of the examined ion were prepared using different dilutions of a multielement standard solution (Multielement standard solution for ICP, TraceCERT, SIGMA-ALDRICH). The cytotoxicity of Fuji II LC and Fuji IX on various cell types have been tested previously [101][102][103][105][106][107]. Only Activa Bioactive restorative and Ena HRi effects on cell survival were investigated.…”

Section: Ion Releasementioning

confidence: 99%

Cytotoxic Evaluation and Determination of Organic and Inorganic Eluates from Restorative Materials

et al. 2021

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Over the last years, diverse commercial resin-based composites have dominated as dental filling materials. The purpose of the present study was to determine organic and inorganic eluates from five restorative materials using GC/MS and ICP–OES and to compare the effect on cell survival of human gingival fibroblasts of a conventional and a bioactive resin. Five commercially available restorative materials were employed for this study: ActivaTM Bioactive Restorative, ENA HRi, Enamel plus HRi Biofunction, Fuji II LC Capsule, and Fuji IX Capsule. Disks that were polymerized with a curing LED light or left to set were immersed in: 1mL methanol or artificial saliva for GC/MS analysis, 5mL deionized water for ICP–OES, and 5mL of culture medium for cell viability. Cell viability was investigated with a modified staining sulforhodamine B assay.The following organic substances were detected: ACP, BHT, BPA, 1,4-BDDMA, CQ, DBP, DMABEE, HEMA, MCE, MeHQ, MOPA, MS, TMPTMA, and TPSb and the ions silicon, aluminum, calcium, sodium, and barium. Activa Bioactive Restorative was found to be biocompatible. Elution of organic substances depended on material’s composition, the nature of the solvent and the storage time. Ions’ release depended on material’s composition and storage time. The newly introduced bioactive restorative was found to be more biocompatible.

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Cytotoxicity of Fast-set Conventional and Resin-modified Glass Ionomer Cement Polymerized at Different Times on SHED

Abstract: RMGIC polymerized at 20 secs exhibited the least favorable cell viability among all groups. Nevertheless, the cell viability was comparable to conventional GICs when the manufacturer's recommended time was doubled (40 secs).

Cited by 5 publications

References 27 publications

In Vitro Evaluation of the Biological Effects of ACTIVA Kids BioACTIVE Restorative, Ionolux, and Riva Light Cure on Human Dental Pulp Stem Cells

In Vitro Evaluation of the Biological Effects of ACTIVA Kids BioACTIVE Restorative, Ionolux, and Riva Light Cure on Human Dental Pulp Stem Cells

Hydroxyapatite decreases cytotoxicity of a glass ionomer cement by calcium fluoride uptake in vitro

Cytotoxic Evaluation and Determination of Organic and Inorganic Eluates from Restorative Materials

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