“…In terms of the material setting condition, 23 studies evaluated root canal sealers in fresh or freshly mixed state, 15 in set condition with 24 h incubation, 15 in both freshly mixed and set conditions, and 20 in a set condition with other or multiple times of incubation. Concerning the cell models used for cell viability assessment (Table A2), several studies used cultures of human cells, namely: dental follicle-derived mesenchymal stem cells [23], tooth germderived stem cells [40], bone marrow-derived mesenchymal stem cells [49], gingival fibroblasts [31,[50][51][52][53][54][55][56], dental pulp stem cells [57], osteoblasts [23,41,46,[58][59][60][61], periodontal ligament cells [26,28,39,45,48,[62][63][64][65][66], human osteoblast-like cells (MG63) [38,42,67,68], cervical carcinoma cells or human cervical carcinoma cells (HeLa) cells [33,37] and THP-1 human monocytic cells [36]. Other cell…”