Cytotoxicity of Different Concentrations of Three Root Canal Sealers on Human Mesenchymal Stem Cells

Alsubait, Sara; Ajlan, Reem Al; Mitwalli, Hala; Aburaisi, Nour; Mahmood, Amer; Manikandan, Muthurangan; Almadhri, Randa; Alfayez, Musaad; Anil, Sukumaran

doi:10.3390/biom8030068

Cited by 82 publications

(92 citation statements)

References 25 publications

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“…In terms of the material setting condition, 23 studies evaluated root canal sealers in fresh or freshly mixed state, 15 in set condition with 24 h incubation, 15 in both freshly mixed and set conditions, and 20 in a set condition with other or multiple times of incubation. Concerning the cell models used for cell viability assessment (Table A2), several studies used cultures of human cells, namely: dental follicle-derived mesenchymal stem cells [23], tooth germderived stem cells [40], bone marrow-derived mesenchymal stem cells [49], gingival fibroblasts [31,[50][51][52][53][54][55][56], dental pulp stem cells [57], osteoblasts [23,41,46,[58][59][60][61], periodontal ligament cells [26,28,39,45,48,[62][63][64][65][66], human osteoblast-like cells (MG63) [38,42,67,68], cervical carcinoma cells or human cervical carcinoma cells (HeLa) cells [33,37] and THP-1 human monocytic cells [36]. Other cell…”

Section: Resultsmentioning

confidence: 99%

Biocompatibility of Root Canal Sealers: A Systematic Review of In Vitro and In Vivo Studies

et al. 2019

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(1) Aim: To perform a systematic review of the literature on the biocompatibility of root canal sealers that encompasses the various types of sealers that are commercially available as well as both in vitro and in vivo evidence. (2) Methods: This systematic review has been registered in PROSPERO (ID 140445) and was carried out according to PRISMA guidelines using the following databases: PubMed, Cochrane Library, ClinicalTrials.gov, Science Direct, and Web of Science Core Collection. Studies published between 2000 and 11 June 2019 that evaluated cytotoxicity (cell viability/proliferation) and biocompatibility (tissue response) of root canal sealers were included. (3) Results: From a total of 1249 studies, 73 in vitro and 21 in vivo studies were included. In general, studies suggest that root canal sealers elicit mild to severe toxic effects and that several factors may influence biocompatibility, e.g., material setting condition and time, material concentration, and type of exposure. Bioactive endodontic sealers seem to exhibit a lower toxic potential in vitro. (4) Conclusions: The available evidence shows that root canal sealers exhibit variable toxic potential at the cellular and tissue level. However, the methodological heterogeneity among studies included in this systematic review and the somewhat conflicting results do not allow a conclusion on which type of sealer presents higher biocompatibility. Further research is crucial to achieve a better understanding of the biological effects of root canal sealers.

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Section: Resultsmentioning

confidence: 99%

Biocompatibility of Root Canal Sealers: A Systematic Review of In Vitro and In Vivo Studies

et al. 2019

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“…, Alsubait et al . ). However, the cell types used in these studies were different, and culture models used were based on cellular contact with extracts, not direct contact which could justify the differing results.…”

Section: Discussionmentioning

confidence: 97%

Evaluation of apoptosis/necrosis and cytokine release provoked by three root canal sealers in human polymorphonuclears and monocytes

Souza¹,

Rosatto²,

Silva³

et al. 2018

Int Endodontic J

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Aim To evaluate the in vitro cytotoxicity and cytokine release of three fresh root canal sealers and to determine the type of cell death they induce. Methodology The sealers tested were Sealer 26 (S26), AH Plus (AHP), and Endosequence BC Sealer (END). Fresh sealers were cultivated in contact with monocytes and polymorphonuclears (PMNs) obtained from the peripheral blood of humans. Cell viability, apoptosis and necrosis were analysed at 4 h (PMNs) or 24 h (monocytes) using Annexin‐V and propidium iodide in a cytometer. The supernatants were used to quantify Interleukin (IL)‐4, IL‐6, IL‐10, IL‐12 and tumour necrosis factor‐α (TNF‐α) in monocytes and IL‐8 in PMNs by ELISA. One‐way ANOVA and the Tukey post‐test were used to compare data for cytotoxicity, and the multiple T‐test was used to determine the differences between sealers in the release of cytokines that were statistically significant. Results After 4 h of treatment, S26 was associated with greater cell viability than the other sealers (P < 0.05) in the PMN culture and had similar values of necrosis as END (P > 0.05). After 24 h of treatment, AHP and END had greater monocyte cell viability than S26 (P < 0.05), which had more necrosis (P < 0.05). END had the lowest levels of IL‐12 compared to the other sealers (P < 0.05) and higher levels of IL‐6 compared to S26 (P < 0.05). The tested sealers did not differ in the release of IL‐8, IL‐10, TNF‐α and IL‐4 (P > 0.05). Conclusions The effect of toxic agents released varied depending on the cell type studied. The composition of the sealers appeared to alter the form of self‐regulation in the production of these cytokines by cells.

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“…The endodontic sealer or elutes, when extruded out of the root canal could induce cytotoxic damage to tissue and may have different level of cytotoxicity over a period of 24 hours or 72 hours (26,27) . So, it is important to evaluate the cytotoxicity of endodontic sealers materials and them elutes after setting over different periods and concentrations because they probably change their cytotoxicity due to diffusion of toxic components resulting from the decomposition of sealers components (1,5,26) .…”

Section: Discussionmentioning

confidence: 99%

“…Clinically, sealers are introduced into canals in a fresh, unset state (5) . They are designed to be confined within the canal; however, they might be extruded beyond the apical constriction, may come in direct contact with periapical tissues and may affect them (1,6,7) .…”

Section: Introductionmentioning

confidence: 99%

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Cytotoxicity of Three Root Canal Sealers on Human Fibroblast Cells (Wi-38) at Different Times and Concentrations

Beshr¹,

Abdelrahim²

2018

Egyptian Dental Journal

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Introduction:The aim of the present study was to evaluate the in vitro cytotoxicity of three endodontic sealers (TotalFill BC, Gutta Flow2 and AH Plus) on WI-38 cell line human. As a reference, AH Plus was compared with the more recent endodontic sealers regarding cell viability of fibroblast cells (WI-38). Methods:Biological testing was carried out in vitro on WI-38 cells. Cell viability assay was performed by using eluates from each endodontic sealer and dispensed to pre-cultured WI-38 cell line. The effect was assessed by MTT cell proliferation assay and ELISA reader after 24 hours and 72 hours of exposure and various concentrations of (0.5, 1,2,4,8,16,32, 64, 125, 250 and 500mg/ ml). Statistical differences were assessed by analysis of variance and Tukey test (P < .05). Results:TotalFill BC, Gutta Flow2 and AH Plus sealer eluates for 24 hours showed no cytotoxic effect, while it was increased by increasing the concentration for 72 hours' measurement. Statistically there is no significant difference among the tested sealer materials; TotalFill BC, Gutta Flow2 and AH Plus. Conclusions:In the present study TotalFill BC, Gutta Flow2 and AH Plus sealer eluates showed no cytotoxic effects at least in the first 24 hours. But they exhibited moderately or severely cytotoxic activity at 72 hours of measurement at high concentration.

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Cytotoxicity of Different Concentrations of Three Root Canal Sealers on Human Mesenchymal Stem Cells

Cited by 82 publications

References 25 publications

Biocompatibility of Root Canal Sealers: A Systematic Review of In Vitro and In Vivo Studies

Biocompatibility of Root Canal Sealers: A Systematic Review of In Vitro and In Vivo Studies

Evaluation of apoptosis/necrosis and cytokine release provoked by three root canal sealers in human polymorphonuclears and monocytes

Cytotoxicity of Three Root Canal Sealers on Human Fibroblast Cells (Wi-38) at Different Times and Concentrations

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