Cytotoxicity of denture base resins: Effect of water bath and microwave postpolymerization heat treatments

Jorge, Janaína Habib; Giampaolo, Eunice Teresinha; Vergani, Carlos Eduardo; Machado, A.L.; Pavarina, Ana Cláudia; Carlos, Iracilda Z.

doi:10.1016/j.prosdent.2004.07.003

Cited by 33 publications

(55 citation statements)

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“…However, when these medical devices were detected by the indirect method, all had a cell toxicity of grade 2. Similar results occurred with assessment of disposable catheters and medical gloves: When the doses of the extract tested were 50-100%, all the cytotoxicity results were grade 4; however, when the dose was 25%, the test results were distributed in grades 4, 3 and 2 (23). Cytotoxicity of an ultrasound-coupling agent showed that the same method of extraction leads to different results as the cell densities were not the same; this was not the case with the indirect method.…”

Section: Detection Methodssupporting

confidence: 74%

Study of the in vitro cytotoxicity testing of medical devices

Li¹,

Zhou²,

Xu³

2015

Biomedical Reports

343

224

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Abstract. The cytotoxicity test is one of the biological evaluation and screening tests that use tissue cells in vitro to observe the cell growth, reproduction and morphological effects by medical devices. Cytotoxicity is preferred as a pilot project test and an important indicator for toxicity evaluation of medical devices as it is simple, fast, has a high sensitivity and can save animals from toxicity. Three types of cytotoxicity test are stated in the International Organization for Standardization 109993-5: Extract, direct contact and indirect contact tests. The xCELLigence real-time cell analysis system shows a significant potential in regards to cytotoxicity in recent years. The present review provides a brief insight into the in vitro cytotoxicity testing of medical devices.

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Section: Detection Methodssupporting

confidence: 74%

Study of the in vitro cytotoxicity testing of medical devices

Li¹,

Zhou²,

Xu³

2015

Biomedical Reports

343

224

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“…In contrast, immersion in water at 55°C had no significant effect on materials cytotoxicity. Similar results were reported by others who found that postpolymerization heat-water treatments did not markedly influence in vitro cytotoxicity regardless of reducing RM content and decreasing the leachability of residual compounds [5, 7, 11–13, 35]. The high decrease of RM content due to ethanol treatment compared to water may partially explain the greatest impact of the novel postpolymerization treatment on materials toxicity.…”

Section: Discussionsupporting

confidence: 88%

Ethanol Postpolymerization Treatment for Improving the Biocompatibility of Acrylic Reline Resins

Neves

Lopes

Ferrão

et al. 2013

BioMed Research International

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Objective. To evaluate the effect of postpolymerization treatment based on ethanol-aqueous solutions on the residual monomer (RM) content, flexural strength, microhardness, and cytotoxicity of hard chairside reline resins (Kooliner, Ufi Gel Hard). Methods. After polymerization, specimens were immersed in water, 20%, 50%, or 70% ethanol solutions at 23°C or 55°C for 10 minutes. Controls were left untreated. HPLC was used for the determination of RM content. Specimens were submitted to Vickers microhardness and 3-point loading flexural strength tests. Cytotoxicity of resin eluates was determined on human fibroblasts by assessing cellular mitochondrial function and lactate dehydrogenase release. Results. Higher concentrations of ethanol promoted lower RM content at 55°C in both materials. The mechanical properties were maintained after 50% and 20% ethanol treatments in Kooliner and Ufi Gel Hard, respectively. Specimens submitted to those treatments showed significant reduction on cytotoxicity compared to immersion in hot water, the treatment of choice in the recent literature. Significance. Immersion of relined dentures in specific ethanol solutions at 55°C for 10 minutes can be considered an effective postpolymerization treatment contributing to increase materials biocompatibility. The proposed protocol is expeditious and easy to achieve with simple equipment in a dental office.

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“…All experiments were performed twice. This protocol was based on the outcomes of previous studies in which the desired reproducibility was assured 17 .…”

Section: Methodsmentioning

confidence: 99%

“…Toxic substances and their effects on the tissues have been observed by animal study, clinical observations and cell cultures in vitro . Testing of dental materials by using cell culture has been proven suitable as an alternative to controversial animal experiments and relatively simple to perform, reproducible and cost‐effective 9,15–17 . Some tests have been performed to determine the cytotoxicity of materials such as the MTT (tetrazolium bromide) and the incorporation of radioactive products such as thymidine, which monitor cell respiration and DNA synthesis, respectively 19–22 .…”

Section: Introductionmentioning

confidence: 99%

Effect of water storage and heat treatment on the cytotoxicity of soft liners

et al. 2011

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Effect of water storage and heat treatment on the cytotoxicity of soft liners Objective: To evaluate the effect of water storage time on the cytotoxicity of soft liners. Methods: Sample discs of soft liners Dentusoft, Dentuflex, Trusoft, Ufi-Gel-P and denture base acrylic resin Lucitone-550 were prepared and divided into four groups: GN: No treatment, G24: Stored in water at 37°C for 24 h; G48: Stored in water at 37°C for 48 h, GHW: Immersed in water at 55°C for 10 min. To analyse the cytotoxic effect, three samples of each group were placed in tubes with Dubelcco's Modified Eagle Mediums and incubated at 37°C for 24 h. During this period, the toxic substances were leached to the culture medium. The cytotoxicity was analysed quantitatively by the incorporation of radioactivity 3 H-thymidine checking the number of viable cells (synthesis of DNA). The data were statistically analysed using two-way ANOVA and Tukey's honestly significant difference tests (a = 0.05). Results: Treatments did not reduce the cytotoxicity effect of the soft liners (p > 0.05). It was found that Ufi-Gel-P had a non-cytotoxic effect, Trusoft had a slightly cytotoxic effect, Dentuflex had a moderated cytotoxic effect, Dentusoft alternated between slightly and non-cytotoxic effect, and Lucitone-550 had non-cytotoxic effect when stored in water for 48 h. Conclusion: The effect of water storage and the heat treatment did not reduce the cytotoxicity of the soft liners.

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Cytotoxicity of denture base resins: Effect of water bath and microwave postpolymerization heat treatments

Cited by 33 publications

References 0 publications

Study of the in vitro cytotoxicity testing of medical devices

Study of the in vitro cytotoxicity testing of medical devices

Ethanol Postpolymerization Treatment for Improving the Biocompatibility of Acrylic Reline Resins

Effect of water storage and heat treatment on the cytotoxicity of soft liners

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