Effect of water storage and heat treatment on the cytotoxicity of soft liners

Abstract

Effect of water storage and heat treatment on the cytotoxicity of soft liners Objective: To evaluate the effect of water storage time on the cytotoxicity of soft liners. Methods: Sample discs of soft liners Dentusoft, Dentuflex, Trusoft, Ufi-Gel-P and denture base acrylic resin Lucitone-550 were prepared and divided into four groups: GN: No treatment, G24: Stored in water at 37°C for 24 h; G48: Stored in water at 37°C for 48 h, GHW: Immersed in water at 55°C for 10 min. To analyse the cytotoxic effect, three samples of each group were placed in tubes with Dubelcco's Modified Eagle Mediums and incubated at 37°C for 24 h. During this period, the toxic substances were leached to the culture medium. The cytotoxicity was analysed quantitatively by the incorporation of radioactivity 3 H-thymidine checking the number of viable cells (synthesis of DNA). The data were statistically analysed using two-way ANOVA and Tukey's honestly significant difference tests (a = 0.05). Results: Treatments did not reduce the cytotoxicity effect of the soft liners (p > 0.05). It was found that Ufi-Gel-P had a non-cytotoxic effect, Trusoft had a slightly cytotoxic effect, Dentuflex had a moderated cytotoxic effect, Dentusoft alternated between slightly and non-cytotoxic effect, and Lucitone-550 had non-cytotoxic effect when stored in water for 48 h. Conclusion: The effect of water storage and the heat treatment did not reduce the cytotoxicity of the soft liners.

Acrylic Resin Cytotoxicity for Denture Base: Literature Review

“…Among them, the most common is the MTT test (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium), which quantifies the mitochondrial succinate dehydrogenase enzyme activity and measures the conversion of water-soluble tetrazolium salt in insoluble blue formazan by spectrophotometry. This test is an excellent marker of cell survival because it evaluates cellular respiratory activity [1,2,5,9,10,12].…”

İki Farklı Yumuşak Astar Materyalinin Sitotoksik Özelliklerinin İncelenmesi

“…Yumuşak astar materyalleri kimyasal yapılarına göre plastik akrilik bazlı rezinler, plastik vinil bazlı rezinler, poliüretan bazlı, polifosfazin bazlı ve silikon bazlı materyaller diye sınıflandırılır (2,3,10,12). Piyasada yaygın olarak kullanılan yumuşak astar materyalleri plastik akrilik rezin bazlı ve silikon bazlı materyallerdir (3,10,11,13).…”

“…Protez kullanımına bağlı olarak oral mukozada reaksiyonların oluşması ile araştırıcılar protez kaide materyallerinin biyolojik davranışlarını araştırmaya yönelmiştir. Yumuşak dokuda en yaygın gözlenen reaksiyonlar enflamasyon, erozyon ve mukoza ve dilde yanmadır (12). Histolojik olarak ise protezle temasta olan mukozada dokularda enflamatuvar infiltrasyon ve keratin artışı gözlenir (12).…”

“…Yumuşak dokuda en yaygın gözlenen reaksiyonlar enflamasyon, erozyon ve mukoza ve dilde yanmadır (12). Histolojik olarak ise protezle temasta olan mukozada dokularda enflamatuvar infiltrasyon ve keratin artışı gözlenir (12). Astar materyalleri içerisindeki etanol ve plastikleştiricilerin materyal yapısından salınması veya materyalin yapısal olarak bozulması çevre ortamdan bir takım maddelerin emilimine neden olur.…”

“…Dental materyallerin hücre kültürü yöntemi ile değerlendirilmesi hayvan çalışmalarına kıyasla daha ucuz, kolay ve tekrar edilebilir bir yöntemdir (12). Dental materyallerin sitotoksisitesinin değerlendirilmesinde MTT testi (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide), XTT testi (sodyum 3'-[1-[(fenilamino)-carbonil]-3,4 tetrazolyum]-bis(4-metoksi-6-nitro) benzen-sulfonik asit hidrat) ve H-timidin testi gibi yöntemlerden faydalanılır (14).…”