Cytotoxicity of dentine-bonding agents on human pulp cells in vitro

Abstract

The influence of the cytotoxicity depended on the materials tested. Dentine-bonding agents have significant potential for pulpal toxicity.

In vitro potential cytotoxicity of an adhesive system to alveolar macrophages

“…In a previous study (14), the adhesive systems evaluated under different culture protocols were cytotoxic to human pulpal cells. The authors alleged that the cause of this cytotoxicity was the presence of substances that can be lixiviated from these materials, and that dentin could be an important pulp protector, in addition to pulp circulation, which reduces the concentration of harmful agents.…”

“…The potential cell damage caused by monomers and other constituents of adhesive systems (7,13,14) may vary from one material to another due to the differences in chemical composition, producing various effects on pulpal tissue (14). Therefore, it is necessary to assess the degree of cellular damage caused by these agents.…”

Theanalysis of cytotoxicity of an experimental preparation used for the reduction of dentin hypersensitivity

“…20 Huang and Chang, as well as Huang et al obtained similar effects using extracts from bonding systems just after photopolymerization and after 16 weeks since their polymerization. 18,19 A number of studies also analyzed binding systems and their effect on the bacterial culture in relation to: the light-curing conditions and their potentially influence on the conversion rate 21 or the level of dilution. 22 Non-diluted extracts had a greater lethal effect on cells than diluted extracts.…”

Polymer Biocompatibility

“…Several types of cells can be used for cytotoxicity tests such as cell lines -human and mouse fibroblasts, lymphocytes, queratinocytes, mouse odontoblast-like cells, mouse macrophages, rat submandibular salivary gland acinar cells - (Bakoupoulou et al, 2007;Franz et al, 2009;Kostoryz et al, 2003;Lin et al, 2007;Roll et al, 2004;Samuelsen et al, 2008), and primary cell types -human lymphocytes, polymorphonuclear leukocytes, and mixed leukocytes, mouse blastocysts, mouse macrophages, and mouse embryo cells - (Becher et al, 2006;Huang & Chang, 2002;Jonhson et al, 1985;Libonati et al, 2011;Porto et al, 2009Porto et al, , 2011Prica et al, 2006).…”