Cytotoxicity of Crude Extract and Isolated Constituents of the<i>Dichrostachys cinerea</i>Bark towards Multifactorial Drug-Resistant Cancer Cells

Mbaveng, Armelle T.; Damen, François; Mpetga, James D. Simo; Awouafack, Maurice D.; Tane, Pierre; Kuete, Victor; Efferth, Thomas

doi:10.1155/2019/8450158

Cited by 27 publications

(15 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Regarding the structure-activity relationship, it appears that pentacyclic triterpene 1 is different from 2 by the presence of the carboxyl group (-COOH) in C-13 ( Figure 1 ); the presence of this −COOH group significantly enhanced the cytotoxic activity of triterpene 1 , with IC 50 values ranging from 14.44 µ M to 44.20 µ M in cancer cells tested whilst no IC 50 value at up to 100 µ M was recorded with triterpene 2 ( Table 1 ). Betulic acid, another pentacyclic triterpene with–COOH in C-17, previously displayed good cytotoxicity against all cancer cell lines tested in this work [ 41 ], illustrating the importance of the carboxyl function in the cytotoxicity of pentacyclic triterpenes. Concerning xanthones, though 5 was active in all tested cancer cell lines, 3-5 displayed moderate activities ( Table 1 ).…”

Section: Discussionmentioning

confidence: 71%

“…To the best of our knowledge, the cytotoxicity of the crude extract and compounds 1 , 2 , 3 , 7 , and 8 on the studied cell lines is being reported, herein, for the first time. Betulinic acid ( 6 ) is a well-known cytotoxic compound and has previously been found active towards the cancer cell lines tested in the present work, with IC 50 values ranging from 7.65 µ M (in CEM-ADR5000 cells) to 44.17 µ M (in HepG2 cells) [ 41 ]. Although it was not further tested, herein, compound 6 can be ranked amongst the best active principles of Hypericum roeperianum.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Cytotoxic Constituents of the Bark of Hypericum roeperianum towards Multidrug‐Resistant Cancer Cells

Guefack

Damen

Mbaveng

et al. 2020

Evidence-Based Complementary and Alternative Medicine

Self Cite

View full text Add to dashboard Cite

The global cancer burden remains a serious concern with the alarming incidence of one in eight men and one in eleven women dying in developing countries. This situation is aggravated by the multidrug resistance (MDR) of cancer cells that hampers chemotherapy. In this study, the cytotoxicity of the methanol extract (HRB), fractions (HRBa, HRBb, and HRBa1-5), and compounds from the bark of Hypericum roeperianum (HRB) was evaluated towards a panel of 9 cancer cell lines. The mode of action of the HRB and trichadonic acid (1) was also studied. Column chromatography was applied to isolate the constituents of HRB. The cytotoxicity of botanicals and phytochemicals was evaluated by the resazurin reduction assay (RRA). Caspase-Glo assay was used to evaluate the activity of caspases, and reactive oxygen species (ROS) (H2DCFH-DA) were assessed by flow cytometry. Phytochemicals isolated from HRB were trichadonic acid (1), fridelan-3-one (2), 2-hydroxy-5-methoxyxanthone (3), norathyriol (4), 1,3,5,6-tetrahydroxyxanthone (5), betulinic acid (6), 3′-hydroxymethyl-2′-(4″-hydroxy-3″,5″-dimethoxyphenyl)-5′,6′:5,6-(6,8-dihydroxyxanthone)-1′,4′-dioxane (7), and 3′-hydroxymethyl-2′-(4″-hydroxy-3″,5″-dimethoxyphenyl)-5′,6′:5,6-(xanthone)-1′,4′-dioxane (8). Botanicals HRB, HRBa, HRBa2-4, HRBb, and doxorubicin displayed cytotoxic effects towards the 9 tested cancer cell lines. The recorded IC50 values ranged from 11.43 µg/mL (against the P-glycoprotein (gp)-overexpressing CEM/ADR5000 leukemia cells) to 26.75 µg/mL (against HCT116 (p53+/+) colon adenocarcinoma cells) for the crude extract HRB. Compounds 1, 5, and doxorubicin displayed cytotoxic effects towards the 9 tested cancer cell lines with IC50 values varying from 14.44 µM (against CCRF-CEM leukemia cells) to 44.20 µM (against the resistant HCT116 (p53−/−) cells) for 1 and from 38.46 µM (against CEM/ADR5000 cells) to 112.27 µM (against the resistant HCT116 (p53−/−) cells) for 5. HRB and compound 1 induced apoptosis in CCRF-CEM cells. The apoptotic process was mediated by enhanced ROS production for HRB or via caspases activation and enhanced ROS production for compound 1. This study demonstrated that Hypericum roeperianum is a potential source of cytotoxic phytochemicals such as trichadonic acid and could be further exploited in cancer chemotherapy.

show abstract

Section: Discussionmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Cytotoxic Constituents of the Bark of Hypericum roeperianum towards Multidrug‐Resistant Cancer Cells

Guefack

Damen

Mbaveng

et al. 2020

Evidence-Based Complementary and Alternative Medicine

Self Cite

View full text Add to dashboard Cite

show abstract

“…Cells were kept at −20 °C for 24 h. After washing with cold PBS, cells were re-suspended in cold PBS with 20 ug/mL RNAse for 1 h at room temperature and then stained with 50 μg/mL propidium iodide (PI) (Thermo Fisher Scientific, Darmstadt, Germany). Cell cycle analysis was performed using the BD Accuri TM C6 Flow Cytometer (BD Biosciences, Heidelberg, Germany) [ 47 , 48 , 49 , 50 ]. Cell cycle data were analyzed with ModFit LT™.…”

Section: Methodsmentioning

confidence: 99%

“…One million of CCRF/CEM or KMS-12-BM cells were exposed to sencha-MeOH and -H 2 O extracts, a solvent control (DMSO), or a positive control hydrogen peroxide (H 2 O 2 ) in a 6-well plate for 48 h. Then, the measurement of ROS production was evaluated with the cell-permeable and non-fluorescent 2′,7′-dichlorodihydrofuorescein diacetate (H2DCFHDA) (Sigma-Aldrich, Taufkirchen, Germany), which could be converted to the highly fluorescent 2′,7′-dichlorofluorescein (DCF) by intracellular hydroxyl and peroxyl. Cells were measured with BD Accuri TM C6 Flow Cytometer using FL-1 the channel (488 nm excitation) [ 48 , 53 ]. 10 4 events were counted for each sample.…”

Section: Methodsmentioning

confidence: 99%

Chemopreventive Property of Sencha Tea Extracts towards Sensitive and Multidrug-Resistant Leukemia and Multiple Myeloma Cells

Saeed

Hegazy

et al. 2020

Biomolecules

Self Cite

View full text Add to dashboard Cite

The popular beverage green tea possesses chemopreventive activity against various types of tumors. However, the effects of its chemopreventive effect on hematological malignancies have not been defined. In the present study, we evaluated antitumor efficacies of a specific green tea, sencha tea, on sensitive and multidrug-resistant leukemia and a panel of nine multiple myelomas (MM) cell lines. We found that sencha extracts induced cytotoxicity in leukemic cells and MM cells to different extents, yet its effect on normal cells was limited. Furthermore, sencha extracts caused G2/M and G0/G1 phase arrest during cell cycle progression in CCRF/CEM and KMS-12-BM cells, respectively. Specifically, sencha-MeOH/H2O extracts induced apoptosis, ROS, and MMP collapse on both CCRF/CEM and KMS-12-BM cells. The analysis with microarray and COMPARE in 53 cell lines of the NCI panel revealed diverse functional groups, including cell morphology, cellular growth and proliferation, cell cycle, cell death, and survival, which were closely associated with anti-tumor effects of sencha tea. It is important to note that PI3K/Akt and NF-κB pathways were the top two dominant networks by ingenuity pathway analysis. We demonstrate here the multifactorial modes of action of sencha tea leading to chemopreventive effects of sencha tea against cancer.

show abstract

“…The crude extract and isolated constituents of the Dichrostachys cinerea bark was also reported against multifactorial drugresistant Cancer Cells by Mbaveng et al, (2019). The betulinic acid and 6-hydroxy-2-(4-hydroxyphenyl)-4H-chromen-4oneisolated from D. cinerashowed cytotoxic effects towards the 9 tested cancer cell lines with IC50 below 50 μM (Mbaveng et al,2019). Tatematsu et al (1991) reported two cytotoxic secondary metabolites isolated from Securinega virosa i.e.…”

mentioning

confidence: 95%

Ethnobotanical Survey of Medicinal Plants Used in the Treatment of Cancer in Dutsin-Ma Local Government Area of Katsina State, Nigeria

Okunola¹,

Ali²,

Bello³

2020

FJS

View full text Add to dashboard Cite

Ethnopharmacological surveys showed that herbal remedies and their products are mostly preferred type of alternative and complementary medicine (ACM) globally. In Dutsin-Ma Local Government Area (LGA) like other places herbal remedies are popularly employed against many ailment including tumor and cancer. Therefore, this study aimed to report and document the ethnobotanical survey of the plants used in the management of tumor and cancer in Dutsin-Ma Local Government Area of Katsina State, Nigeria. Traditional medicine practitioners (TMPs) living within the area of study were cross-examined by employing questionnaires forms and key informant survey. Forty-two (42) different plant species were discovered to be employed against cancer and tumor management. The results obtained showed the parts of plant used for the herbs include; roots (4), bark (10), whole plant (13), seeds (5), fruits (3), stem (1) and leaves (6). The plant families Leguminosae caesalpinoideae and Leguminosae mimosoideae appeared more in the ethno-surveyed plant list though other families are involved. Many of these plants are mix together as storehouses for active compounds which may be good template for cancer and tumor management. The preparation of the remedies include; grinding to powder form then dispersing in either water or milk for drinking, or either mix with shear butter or water and administered on the affected part of the body. The reports showed that the herbal remedies indentified are effective for the treatment of cancer in Dutsin-Ma local government area. Hence, it is imperative for ethnobotanists and chemists to establish the toxicity and efficacy of these

show abstract

Cytotoxicity of Crude Extract and Isolated Constituents of theDichrostachys cinereaBark towards Multifactorial Drug-Resistant Cancer Cells

Cited by 27 publications

References 33 publications

Cytotoxic Constituents of the Bark of Hypericum roeperianum towards Multidrug‐Resistant Cancer Cells

Cytotoxic Constituents of the Bark of Hypericum roeperianum towards Multidrug‐Resistant Cancer Cells

Chemopreventive Property of Sencha Tea Extracts towards Sensitive and Multidrug-Resistant Leukemia and Multiple Myeloma Cells

Ethnobotanical Survey of Medicinal Plants Used in the Treatment of Cancer in Dutsin-Ma Local Government Area of Katsina State, Nigeria

Contact Info

Product

Resources

About