Chemopreventive Property of Sencha Tea Extracts towards Sensitive and Multidrug-Resistant Leukemia and Multiple Myeloma Cells

Abstract

<jats:p>The popular beverage green tea possesses chemopreventive activity against various types of tumors. However, the effects of its chemopreventive effect on hematological malignancies have not been defined. In the present study, we evaluated antitumor efficacies of a specific green tea, sencha tea, on sensitive and multidrug-resistant leukemia and a panel of nine multiple myelomas (MM) cell lines. We found that sencha extracts induced cytotoxicity in leukemic cells and MM cells to different extents, yet its effect on normal cells was limited. Furthermore, sencha extracts caused G2/M and G0/G1 phase arrest during cell cycle progression in CCRF/CEM and KMS-12-BM cells, respectively. Specifically, sencha-MeOH/H2O extracts induced apoptosis, ROS, and MMP collapse on both CCRF/CEM and KMS-12-BM cells. The analysis with microarray and COMPARE in 53 cell lines of the NCI panel revealed diverse functional groups, including cell morphology, cellular growth and proliferation, cell cycle, cell death, and survival, which were closely associated with anti-tumor effects of sencha tea. It is important to note that PI3K/Akt and NF-κB pathways were the top two dominant networks by ingenuity pathway analysis. We demonstrate here the multifactorial modes of action of sencha tea leading to chemopreventive effects of sencha tea against cancer.</jats:p>

Increased Stress Resistance and Lifespan in Chaenorhabditis elegans Wildtype and Knockout Mutants—Implications for Depression Treatment by Medicinal Herbs

“…The following method has been applied with the same equipment and similar settings, as previously reported by us [ 49 ]. Dried extracts were dissolved in MeOH at a concentration of 2 mg/mL and diluted by a factor of 1000.…”

Molecular mechanisms and applications of tea polyphenols: A narrative review

“…TPs have been shown to have strong anti-tumor effects in many studies (Lu et al, 2020;Rady et al, 2018). In general, the anti-tumor mechanisms of TPs can be summarized as: (Hu et al, 2014;Min & Kwon, 2014;Rashidi et al, 2017;Tang et al, 2017;Wang, Pan et al, 2018;Wang, Xiong, et al, 2019).…”

“…Studies have been shown that EGCG inhibits STAT and Pglycoprotein signaling pathways to suppress cell proliferation and drug resistance in breast cancer cell (Lu et al, 2020;Nakano et al, 2018;Tang et al, 2017). GTPs can regulate the transition between pre-DNA synthesis/DNA synthesis (G1/S) and late DNA synthesis/division (G2/M), inducing cell cycle arrest and significantly inhibiting the growth of MCF-7 cells (Wang, Pan, et al, 2018;Wang, Cui, et al, 2020).…”

“…Meanwhile, GTPs also induce apoptosis through the induction of the mitochondrial apoptotic pathway, in which the depolarization of mitochondrial membrane potential (ΔΨm) is an early sign of apoptotic signal activation. Excessive generation of ROS leads to oxidative damage to mitochondrial membrane, which ultimately affects apoptosis (Hayakawa et al, 2020;Liu, Ou, et al, 2017;Lu et al, 2020). Furthermore, in non-small cell lung cancer, EGCG/GTPs pretreatment leads to a decrease in mRNA and protein levels of interferon IFNγ-induced programmed cell death ligand 1 (PD-L1) by inhibiting Janus kinase/signal transducer and activator of transcription (STAT) signal transduction; EGCG pretreatment also reduces the expression of EGF-induced PD-L1 by inhibiting epidermal growth factor receptor (EGFR)/AKT signaling (Yang et al, 2009).…”

The Effect of Yellow Tea Leaves Camellia sinensis on the Quality of Stored Chocolate Confectionery

“…Numerous studies reported that regular intake of tea components is associated with an improved antioxidant status in vivo conditions. Systematic delivery of substances with high antioxidant potential may contribute to the lowering risk of coronary heart disease, stroke, atherosclerosis, certain types of cancer, increasing insulin sensitivity properties, and protection against inflammation, bacteria or neurodegenerative diseases [22,[28][29][30][31][32][33].…”