Cytotoxicity of Aspergillus strains isolated from the neonatal intensive care unit environment

Gniadek, Agnieszka; Macura, Anna B.; Twarużek, Magdalena; Grajewski, Jan

doi:10.2478/v10039-010-0039-y

Cited by 10 publications

(21 citation statements)

References 16 publications

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“…Also, the results of this study are consistent with a study conducted by Gniadek et al (2010) [19] with respect to non-Candida species as an etiology for IFD. The authors found an increased prevalence of environmental contamination and colonization of individuals working with infants in pediatric and neonatal intensive care units, primarily by cytotoxic strains of Aspergillus spp., in particular A. fumigatus.…”

Section: Discussionsupporting

confidence: 91%

Etiology of Fungemia in Neonates: Four Decades of Case Reports

Amaral-Lopes

Tavares-Neto

2014

OJMM

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Objectives: To compare groups of etiologic agents for neonatal fungemia based on previously published case reports with regard to case outcomes. Methods: Secondary analysis of data from case reports published in scientific literature indexed in Medline, Scopus and LILACS databases, and starting from the year, full texts were registered until the year 2010. Results: Thirty-four species of fungi were isolated in 252 cases reported over a 40-year period. The distribution of fungal groups according to the development level of a case's region of origin was very uneven (p < 0.001), with a predominance of C. albicans and other fungi (Aspergillus sp.) in more developed countries. However, 73% of the chi-squared distribution corresponded to differences observed in the frequency of Candida sp. and non-albicans Candida. While the frequency of candidemia by C. albicans was progressively declining in the period from 1966 to 2010, reports of non-albicans candidemia and other non-Candida fungi became significantly (p < 0.00001) more frequent over the course of the period studied. As for the outcome of death, it was statistically more frequent (p < 0.02) in cases where non-albicans Candida or non-Candida fungi were isolated. Conclusion: fungi of the genera Candida and Aspergillus were the etiologic agents most frequently identified in published cases of neonatal fungemia; the outcome of death was more often related to cases where non-albicans Candida or non-Candida fungi were isolated.

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Section: Discussionsupporting

confidence: 91%

Etiology of Fungemia in Neonates: Four Decades of Case Reports

Amaral-Lopes

Tavares-Neto

2014

OJMM

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“…Open Petri dishes were exposed to air for between 10 and 30 min at a height of 80 to 150 cm from the floor. However, most authors employed air vacuum devices such as Anderson single-step model (10-710), MAS 100 Iso MH Microbial Air Sampler (Merck Millipore, Germany), SAS SUPER 100 (Merck), and LWC (Kangjie Instrument Research Institute, Liaoyang, China) at a variable flow rate (28.3-100 L/min) to obtain the suspended particle concentration (Martins-Diniz et al, 2005;Krajewska-Kułak et al, 2007;Chuaybamroong et al, 2008;Quadros et al, 2009;Ortiz et al, 2009;Qudiesat et al, 2009;Gniadek et al, 2010;Kim et al, 2010;Boff et al, 2013;Macedo et al, 2013;Park et al, 2013;Hoseinzadeh et al, 2013;El-Sharkawy & Noweir, 2014;Maldonado-Vega et al, 2014;Yu et al, 2015;Gniadek et al, 2017;Abbasi & Samaei, 2019). In some studies, material from inanimate surfaces (keyboards, phones, stethoscope, beds, door handles, walls, and equipment) was also collected by means of swabs or imprints (Martins-Diniz et al, 2005;Krajewska-Kułak et al, 2007;Melo et al, 2009;Qudiesat et al, 2009;Sales et al, 2011;Aboul-Nasr et al, 2014).…”

Section: Collection and Cultivation Techniquesmentioning

confidence: 99%

“…constituted 70.5% of the recovered filamentous fungi in a study conducted in Greece (Krajewska-Kułak et al, 2007) whist in a Brazilian study (Mobin & Salmito, 2006), A. niger was identified in 60% of the isolates obtained from the indoor air in the ICUs of public and private hospitals in Teresina, PI. Qudiesat et al (2009) reported that Aspergillus was the main fungus recovered from neonatal wards in public and private hospitals in Zarqa, Jordan, whereas Gniadek et al (2010), reported that A. fumigatus was the most frequently isolated Aspergillus species from the indoor air, walls, and equipment in a NICU. A Brazilian study reported recovering the same Aspergillus species from the indoor air from four of ten investigated ICUs (Mobin & Salmito, 2006).…”

Section: Identification and The Main Isolated Organismsmentioning

confidence: 99%

Fungi in the indoor air of critical hospital areas: a review

2021

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Invasive fungal infection is an important cause of mortality and morbidity in neonates, especially in low-birthweight neonates. The contribution of fungi in the indoor air to the incidence of mucocutaneous colonization and to the risk of invasive fungal infection in this population is uncertain. This review aimed to identify and to summarize the best available evidence on the fungal contamination in the indoor air of critical hospital areas with an emphasis on pediatric/neonatal ICUs. Publications from 2005 to 2019 were searched in the databases Scientific Electronic Library Online (SciELO), US

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“…For the cells damaged or destroyed by mycotoxins, the reaction is less intensive or does not occur at all (Hanelt et al 1994). The SK cells (2.2 9 10 5 ) were cultivated in a mixture of ethanoldimethyl sulphoxide-minimum medium with Earle's salts (MEM) supplemented with antibiotics (penicillin and streptomycin; Sigma-Aldrich, Poznań, Poland) and foetal calf serum (Sigma-Aldrich) according to the procedure described by Gniadek et al (2010). Chloroform spore extracts of the fungal strains were added to the medium forming 1:2 dilution series with the tested concentrations ranged between 31.25 and 0.061 cm 2 mL -1 .…”

Section: Detection Of Cytotoxic Strains Of a Fumigatusmentioning

confidence: 99%

Fungal air contamination in distinct sites within a municipal landfill area

Frączek

Kozdrój

Górny

et al. 2017

Int. J. Environ. Sci. Technol.

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Municipal wastes collected in landfills are a significant source of air contamination and frequently characterize by elevated concentrations of different fungi. Posing a serious health threat to landfill workers and local residents, the fungal aerosol has to be monitored with respect to its quantity and quality. In this study, concentrations of airborne fungi, their particle size distribution, species composition and the presence of cytotoxic strains of Aspergillus fumigatus were assessed in different sites within the landfill area. The quantitative and qualitative changes in the fungal aerosol were determined with respect to a season and landfill activity level (i.e. exploitation or standstill periods). Within the landfill area, particular sites were grouped with regard to airborne fungi concentrations and similarities in species composition. The qualitative analysis indicated that 43 species were shared during both sampling times, and only nine species were characteristic for the standstill period. Among fungal isolates, 21 strains of A. fumigatus revealed cytotoxic activity expressed at different levels, depending on the fungal extract concentrations used in the MTT assay. The results suggested that exposure (especially in summer) to small airborne particles, containing distinct species, may occur not only in the active sector but also in close vicinity to the landfill. Hence, microbial monitoring of the landfill and surrounding area should be carried out taking into account both quantitative aspect supplemented by size distribution analysis and qualitative features, especially of those strains possessing cytotoxic activity.

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Cytotoxicity of Aspergillus strains isolated from the neonatal intensive care unit environment

Cited by 10 publications

References 16 publications

Etiology of Fungemia in Neonates: Four Decades of Case Reports

Etiology of Fungemia in Neonates: Four Decades of Case Reports

Fungi in the indoor air of critical hospital areas: a review

Fungal air contamination in distinct sites within a municipal landfill area

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