Cytotoxicity of antineoplastic agents on rabbit corneal epithelium in vitro

Botti, Roberte.; Hillard, M. Lazarus; Paul, Saki; H, Jonathan

doi:10.3109/15569528709052167

Cited by 11 publications

(5 citation statements)

References 25 publications

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“…33 Antiproliferative effects have also been shown on rabbit corneal epithelial cells, 29,32,34 bovine corneal endothelial cells, 35 rabbit lens epithelial cells, 36 and human retinal pigment epithelial cells. 37 However, these studies have never been conducted in the same experimental conditions.…”

Section: Discussionmentioning

confidence: 94%

“…A collagen shield implant containing 5-FU caused inflammatory reaction and capsule formation around the implant. 46 A hydrophobic poly(ortho ester) and 5-FU sys- 29 Rabbit corneal epithelial cells 6 ϫ 10 Ϫ4 5 days Coulter counter Rivera et al 32 Rabbit corneal epithelial cells 4.2 ϫ 10 Ϫ4 5 days Hemocytometer/cell counter Botti et al 34 Rabbit corneal epithelial cells Significant inhibition 1.3 ϫ 10 Ϫ4…”

Section: Discussionmentioning

confidence: 99%

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The Effects of 5-Fluorouracil on Ocular Tissues In Vitro and In Vivo after Controlled Release from a Multifunctional Implant

Huhtala

Rönkkö²,

Teräsvirta³

et al. 2009

Invest. Ophthalmol. Vis. Sci.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

The Effects of 5-Fluorouracil on Ocular Tissues In Vitro and In Vivo after Controlled Release from a Multifunctional Implant

Huhtala

Rönkkö²,

Teräsvirta³

et al. 2009

Invest. Ophthalmol. Vis. Sci.

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“…5-FU treatment has been found to be associated with various types of ocular toxicity, affecting mainly the corneal epithelium [50] . Previous invitro studies on the human as well as rabbit corneal epithelial cells reported that 5-FU has anti-proliferative effects [51,52] .…”

Section: Discussionmentioning

confidence: 99%

The Potential Protective Role of Hesperidin Against Capecitabine-Induced Corneal Toxicity in Adult Male Albino Rat. Light and Electron Microscopic Study

Elwan

Kassab

2017

Egyptian Journal of Histology

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Background: Capecitabine is a chemotherapeutic agent widely used for the treatment of malignancies. Ocular disorders have been reported with capecitabine therapy. Hesperidin is a naturally occurring compound derived mainly from citrus fruits and has a wide range of pharmacological activities and a proposed role in combating many ocular diseases. Aim: To evaluate the potential protective role of hesperidin against the corneal toxicity caused by capecitabine in adult male albino rats. Material and Methods: Thirty-six adult male albino rats were divided into four equal groups; control group, hesperidintreated group (50 mg/kg), capecitabine-treated group (40 mg/kg), and combination-treated "capecitabine and hesperidintreated" group. Animals were orally administered once daily for one month. Specimens from the cornea were processed for light and electron microscopy. Immunohistochemical study was performed using antibodies against p53. Results: Specimens from capecitabine-treated animals showed significant decrease of epithelial thickness. The corneal epithelial cells showed nuclear alteration and vacuolated cytoplasm. The stromal collagen fibers were irregularlyarranged and widely separated with neovascularization and mononuclear cellular infiltration. Ultrastructurally, focal widening of the intercellular spaces, partial loss of desmosomal junctions and swollen mitochondria were observed. The immunohistochemical study showed a significant increase in p53 immunoreaction. In contrast, minimal changes were observed in rats treated concomitantly with both capecitabine and hesperidin, with a non significant increase in the immunoreactions. Conclusion: Capecitabine induced structural changes in cornea of adult albino rat that could be ameliorated by concomitant treatment with hesperidin.

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“…[5 -9,19] 5-FU has been reported to cause antiproliferative effects in vitro in rabbit conjunctival fibroblasts, [10 -13] human scleral fibroblasts, [4] and human Tenon's capsule fibroblasts. [20] 5-FU has also been shown to induce antiproliferative effects in other ocular cells, such as rabbit corneal epithelial cells, [4,11,14] human retinal pigment epithelial cells, [21] rabbit lens epithelial cells, [22] and bovine corneal endothelial cells. [23] The EC 50 values and the method used for evaluation of the antiproliferative effect of 5-FU are presented in Table 1.…”

Section: Huhtala Et Al 288mentioning

confidence: 99%

“…[5 -9] Cytotoxicity of 5-FU in vitro has been studied previously by using rabbit conjunctival fibroblasts [10 -13] and rabbit corneal epithelial cells. [4,11,14] In the present study, the direct adverse effects of 5-FU on SV40-immortalized human corneal epithelial (HCE) cells were evaluated. Special attention was paid to differentiate the effects on cell proliferation and viability.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Adverse Ocular Effects of 5-Fluorouracil by Using Human Corneal Epithelial Cell Cultures

Huhtala

Tähti

Salminen

et al. 2002

Journal of Toxicology: Cutaneous and Ocular Toxicology

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5-Fluorouracil (5-FU) is commonly used in ophthalmology for suppressing fibroblast activity after glaucoma surgery. Adverse effects on corneal epithelial cells have been reported to relate to 5-FU therapy. The effects of 5-FU were evaluated in vitro on SV40-immortalized human corneal epithelial cell (HCE) cultures with two cytotoxicity tests: WST-1 assay as an index of cell proliferation, and lactate dehydrogenase (LDH) assay as an index of plasma membrane integrity. The cells were exposed to 5-FU with various concentrations in serum-free medium and in medium containing 15 % (v/v) fetal bovine serum (FBS) for 1, 24, 48 and 72 hours. One-hour exposure had no effects on HCE cells. Longer exposures caused dose-dependent inhibition of cell proliferation. Exposure to 5 mg/ml 5-FU lowered cell number to 50 % of controls after 24-hour treatment and resulted to complete cell death after 72 hours. Serum protected the cells for 24 hours, but after longer exposure times the protective nature of serum disappeared. 5-FU had only minor effects on LDH release. The LDH leakage was at its peak after 48-hour treatment.

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Cytotoxicity of antineoplastic agents on rabbit corneal epithelium in vitro

Cited by 11 publications

References 25 publications

The Effects of 5-Fluorouracil on Ocular Tissues In Vitro and In Vivo after Controlled Release from a Multifunctional Implant

The Effects of 5-Fluorouracil on Ocular Tissues In Vitro and In Vivo after Controlled Release from a Multifunctional Implant

The Potential Protective Role of Hesperidin Against Capecitabine-Induced Corneal Toxicity in Adult Male Albino Rat. Light and Electron Microscopic Study

Evaluation of Adverse Ocular Effects of 5-Fluorouracil by Using Human Corneal Epithelial Cell Cultures

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