Cytotoxicity of 2-tert-butyl hydroquinone glutathione conjugates after apical and basolateral exposure of rat renal proximal tubular cell monolayers

Haenen, H.E.M.G.; Bleijlevens, E.; Elzerman, H.; Temmink, J.H.M.; Koeman, J.H.; Bladeren, P.J. van

doi:10.1016/0887-2333(95)00116-6

Cited by 5 publications

(2 citation statements)

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“…who found loss of cell viability and LDH release in renal proximal tubular cells after 250 μM and 500 μM HQ treatment. [24] Our findings differ from those of Terasaka el al. who reported caspase-3 and caspase-9 activation at 50 μM HQ in HL60 cells after 6 h of incubation.…”

Section: Discussioncontrasting

confidence: 99%

Effects of hydroquinone on retinal and vascular cells in vitro

Sharma

Patil

Gramajo

et al. 2012

Indian J Ophthalmol

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Aim:To explore the molecular pathophysiology that might explain the epidemiologic association between cigarette smoke and age-related macular degeneration (AMD) by examining the effects of hydroquinone (HQ), a toxic compound present in high concentration in cigarette smoke-related tar, on human retinal pigment epithelial cells (ARPE-19), rat retinal neurosensory cells (R-28), and human microvascular endothelial cells (HMVEC).Materials and Methods:ARPE-19, R-28, and HMVEC were treated for 24 h with four different concentrations of HQ (500 μM, 200 μM, 100 μM, 50 μM). Cell viability, caspase-3/7 activation, DNA laddering patterns, and lactate dehydrogenase (LDH) levels were analyzed.Results:At 50 μM HQ, R-28 cells showed a significant decrease in cell viability compared with the dimethyl sulfoxide (DMSO)-treated controls. At the 100–500 μM concentrations, all three cell lines showed significant cell death (P < 0.001). In the ARPE-19, R-28, and HMVEC cultures, the caspase-3/7 activities were not increased at any of the HQ concentration.Conclusion:Our findings suggest that the mechanism of cell death in all three cell lines was through non-apoptotic pathway. In addition, neuroretinal R-28 cells were more sensitive to HQ than the ARPE-19 and HMVEC cultures.

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Section: Discussioncontrasting

confidence: 99%

Effects of hydroquinone on retinal and vascular cells in vitro

Sharma

Patil

Gramajo

et al. 2012

Indian J Ophthalmol

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“…Nowadays, many synthetic antioxidants like butylated hydroxyl anisole (BHA), butylated hydroxyl toluene (BHT), proppylgallate (PG), and tert-butylhydroquinone (TBHQ) represent strong antioxidant activities and, therefore, are being used to remove free radicals in food and biological systems (Haenen et al, 1996). However, because of the potential side effects, their applications are strictly controlled (Chen et al, 1992).…”

Section: Introductionmentioning

confidence: 99%