An identified antioxidant peptide obtained from ostrich (<i>Struthio camelus</i>) egg white protein hydrolysate shows wound healing properties

Tabrizi, Masoud Homayouni; Asoodeh, Ahmad; Abbaszadegan, M R; Shahrokhabadi, Khadijeh; Moghaddam, Mahboobeh Nakhaie

doi:10.3109/13880209.2014.962061

Cited by 17 publications

(6 citation statements)

References 39 publications

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“…The 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical was used to test the antioxidant activities of the pGO and the pGO-CS/SF scaffolds, based on previously reported methods. , First, DPPH was dissolved in a methanol solution to prepare a 100 μM solution for use. pGO, hGO, and pGO-CS/SF scaffolds were dispersed in a methanol solution to obtain dispersions of 1 and 100 mg/mL.…”

Section: Materials and Experimental Sectionmentioning

confidence: 99%

Mussel-Inspired Electroactive and Antioxidative Scaffolds with Incorporation of Polydopamine-Reduced Graphene Oxide for Enhancing Skin Wound Healing

Tang

Han

et al. 2019

ACS Appl. Mater. Interfaces

179

139

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Wound repair and tissue regeneration are complex processes that involve many physiological signals. Thus, employing novel wound dressings with potent biological activity and physiological signal response ability to accelerate wound healing is a possible solution. Herein, inspired by mussel chemistry, we developed a polydopamine (PDA)-reduced graphene oxide (pGO)-incorporated chitosan (CS) and silk fibroin (SF) (pGO-CS/SF) scaffold with good mechanical, electroactive, and antioxidative properties as an efficient wound dressing. First, pGO with good dispersibility and cell affinity was obtained upon reduction by PDA under alkali conditions. Second, pGO was dispersed into a CS/SF mixture, and then CS and SF chains were dual-cross-linked by poly(ethylene glycol) diglycidyl ether and glutaraldehyde to obtain a pGO-incorporated gel. Finally, the gel underwent a freeze-dry process to obtain the pGO-CS/SF scaffold. Owing to PDA reduction and functionalization, pGO in the scaffold plays important roles for the performances of the scaffolds. First, the pGO acts as nanoreinforcement to enhance the mechanical properties of the scaffold by combining the dual-cross-linked CS/SF network. Second, the uniformly distributed pGO in the scaffolds comprises a well-connected electric pathway, which can provide a channel for the transmission of electrical signals in the scaffold. Moreover, pGO in the scaffolds serves as an antioxidant agent to scavenge reactive oxygen species (ROS) and therefore terminates excessive ROS oxidation. In vitro studies show that electroactive pGO-CS/SF scaffolds can respond to electrical signals and promote cytological behavior. In addition, the pGO-CS/SF scaffolds can reduce cellular oxidation by removing excessive ROS. The in vivo full-thickness skin defect model demonstrates that the electroactive and antioxidative pGO-CS/SF scaffold can efficiently enhance wound healing. In summary, the pGO-CS/SF scaffold is a promising wound dressing because of its ability to promote physiological electrical signal transmission for cell growth and reduce ROS oxidation, resulting in an improved wound regeneration effect.

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Section: Materials and Experimental Sectionmentioning

confidence: 99%

Mussel-Inspired Electroactive and Antioxidative Scaffolds with Incorporation of Polydopamine-Reduced Graphene Oxide for Enhancing Skin Wound Healing

Tang

Han

et al. 2019

ACS Appl. Mater. Interfaces

179

139

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“…In a mouse model of burn injury, the antioxidant peptide SS31 alleviated symptoms of burn injury and promoted recovery of skeletal muscle mitochondrial functions [82,83]. An antioxidant peptide identified from ostrich egg white protein hydrolysate was also shown to promote wound healing in rats [84]. At present, the effects of pure antioxidant peptides identified from edible marine invertebrates have not been tested in animal models of human diseases.…”

Section: Potential Applications In Food Therapy and Cosmeticsmentioning

confidence: 99%

Enzyme-Assisted Discovery of Antioxidant Peptides from Edible Marine Invertebrates: A Review

et al. 2017

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Marine invertebrates, such as oysters, mussels, clams, scallop, jellyfishes, squids, prawns, sea cucumbers and sea squirts, are consumed as foods. These edible marine invertebrates are sources of potent bioactive peptides. The last two decades have seen a surge of interest in the discovery of antioxidant peptides from edible marine invertebrates. Enzymatic hydrolysis is an efficient strategy commonly used for releasing antioxidant peptides from food proteins. A growing number of antioxidant peptide sequences have been identified from the enzymatic hydrolysates of edible marine invertebrates. Antioxidant peptides have potential applications in food, pharmaceuticals and cosmetics. In this review, we first give a brief overview of the current state of progress of antioxidant peptide research, with special attention to marine antioxidant peptides. We then focus on 22 investigations which identified 32 antioxidant peptides from enzymatic hydrolysates of edible marine invertebrates. Strategies adopted by various research groups in the purification and identification of the antioxidant peptides will be summarized. Structural characteristic of the peptide sequences in relation to their antioxidant activities will be reviewed. Potential applications of the peptide sequences and future research prospects will also be discussed.

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“…As shown in Figure 1B , the deduced sequence from the fragment ions (b and y fragments) was found to have the sequence of WESLSRLLG (called WG-9) with the molecular weight of 1060.22 Da. We previously purified and identified a bioactive peptide named DG-10 from ostrich egg hydrolysate showing wound-healing properties [ 24 ]. Here, the antioxidant and ACE inhibitory activity of the WG-9 peptide was reported.…”

Section: Resultsmentioning

confidence: 99%

Biochemical characterization of a novel antioxidant and angiotensin I-converting enzyme inhibitory peptide from Struthio camelus egg white protein hydrolysis

Asoodeh

Tabrizi

Shabestarian

et al. 2016

Journal of Food and Drug Analysis

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A peptide from ostrich (Struthio camelus) egg white protein hydrolysate (OEWPH) was purified, characterized, and its antioxidant and enzyme inhibitory properties were evaluated. The OEWPH was prepared using pepsin and pancreatin, and then fractionated using reversed-phase high performance liquid chromatography. The antioxidant activity of the WG-9 peptide was investigated based on its scavenging capacity for 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, 2,20-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), superoxide (O), hydroxyl (OH), and lipid peroxidation inhibition. The angiotensin-converting enzyme (ACE) inhibitory activity and kinetic parameters of the peptide were determined using N-[3-(2-Furyl)acryloyl]-L-phenylalanyl-glycyl-glycine (FAPGG) as a substrate. Tandem mass spectrometry analysis of the purified peptide revealed a sequence of WESLSRLLG (MW: 1060 Da; WG-9). This peptide inhibited linoleic acid oxidation and acted as a DPPH (IC = 15 ± 0.4 μg/mL), ABTS (IC = 130 ± 4.5 μg/mL), superoxide (IC = 160 ± 6.4 μg/mL), and hydroxyl (IC = 150 ± 6.7 μg/mL) radical scavenger. The ACE-inhibitory activity and kinetic parameters of the WG-9 peptide were determined, showing an ACE inhibitory activity with IC of 46.7 ± 1.4 μg/mL. The parameters of peptide/ACE interactions were investigated by molecule docking. Furthermore, viability assays showed that the identified peptide had no cytotoxicity against an HFLF-PI-5 cell line. In conclusion, the WG-9 peptide showed potent antioxidant and ACE-inhibitory activity.

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An identified antioxidant peptide obtained from ostrich (Struthio camelus) egg white protein hydrolysate shows wound healing properties

Abstract: Our results suggested that DG-10 is a natural agent obtained from ostrich egg possessing considerable antioxidant and wound-healing properties.

Cited by 17 publications

References 39 publications

Mussel-Inspired Electroactive and Antioxidative Scaffolds with Incorporation of Polydopamine-Reduced Graphene Oxide for Enhancing Skin Wound Healing

Mussel-Inspired Electroactive and Antioxidative Scaffolds with Incorporation of Polydopamine-Reduced Graphene Oxide for Enhancing Skin Wound Healing

Enzyme-Assisted Discovery of Antioxidant Peptides from Edible Marine Invertebrates: A Review

Biochemical characterization of a novel antioxidant and angiotensin I-converting enzyme inhibitory peptide from Struthio camelus egg white protein hydrolysis

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