Cytotoxicity, inhibition of DNA and protein syntheses and oxidative damage in cultured cells exposed to zearalenone

Abid‐Essefi, Salwa; Ouanes, Zouhour; Hassen, Wafa; Baudrimont, Isabelle; Creppy, E. E.; Bacha, Hassen

doi:10.1016/j.tiv.2003.12.011

Cited by 228 publications

(145 citation statements)

References 46 publications

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“…For instance, ZEN has been demonstrated to be hepatotoxic (Maaroufi et al 1996;Obremski et al 1999;Conkova et al 2001;Bouaziz et al 2008), hematotoxic (Maaroufi et al 1996;Murata et al 2003;Abbes et al 2006a, b), nephrotoxic Abbes et al 2006a;Liang et al 2010), and toxic toward the intestinal tract (Abid-Essefi et al 2003). Different reports have shown in vitro and in vivo that ZEN induces cytotoxicity through reactive oxygen species (ROS) production leading to lipid peroxidation, DNA damages, and apoptosis by the mitochondrial pathway (Abid-Essefi et al 2004;Hassen et al 2007;Abbes et al 2007;Bouaziz et al 2008). ZEN was also shown to induce endoplasmic reticulum (ER) stress-mediated apoptosis in leukemic cells (Banjerdpongchai et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Crocin and Quercetin protect HCT116 and HEK293 cells from Zearalenone-induced apoptosis by reducing endoplasmic reticulum stress

Salem

Prola

Boussabbeh

et al. 2015

Cell Stress and Chaperones

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Mycotoxins are considered to be significant contaminants of food and animal feed. Zearalenone (ZEN) is a non-steroidal estrogenic mycotoxin produced by several species of Fusarium in cereals and agricultural products. ZEN has been shown to be cytotoxic, genotoxic, and mutagenic in different cell types. In the present study, we investigated the involvement of endoplasmic reticulum (ER) stress in ZENmediated toxicity in human intestine (HCT116) and kidney (HEK293) cells and evaluated the effects of the two common dietary compounds Quercetin (QUER) and Crocin (CRO). We show that ZEN treatment induces ER stress and activates the unfolded protein response (UPR) as evidenced by XBP1 mRNA splicing and upregulation of GRP78, ATF4, GADD34, PDIA6, and CHOP. Activation of the ER stress response is associated with activation of the mitochondrial pathway of apoptosis. This apoptotic process is characterized by an increase in ROS generation and lipid peroxidation, a loss of mitochondrial transmembrane potential (ΔΨm), and an activation of caspases and DNA damages. We also demonstrate that the antioxidant properties of QUER and CRO help to prevent ER stress and reduce ZEN-induced apoptosis in HCT116 and HEK293 cells. Our results suggest that antioxidant molecule might be helpful to prevent ZEN-induced ER stress and toxicity.

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Section: Introductionmentioning

confidence: 99%

Crocin and Quercetin protect HCT116 and HEK293 cells from Zearalenone-induced apoptosis by reducing endoplasmic reticulum stress

Salem

Prola

Boussabbeh

et al. 2015

Cell Stress and Chaperones

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“…Também pode ser observada redução na taxa de concepção acompanhada de repetição de cio, nascimento de leitões fracos e natimortos e, muitas vezes, surtos da síndrome dos membros abertos (Malekinejad et al, 2006). Embora pouco explorados, os efeitos tóxicos da ZEA interferem na síntese lipídica e protéica dos animais (Szkudelska, 2002;Abid-Essefi et al, 2004). Assim, suínos poderiam apresentar alterações no seu desenvolvimento quando expostos à micotoxina.…”

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Alimentação de leitoas pré-púberes com dietas contendo zearalenona

Andretta

Lovatto

Hauschild

et al. 2008

Arq. Bras. Med. Vet. Zootec.

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RESUMOO desempenho, o peso de alguns órgãos e a morfologia vulvar de leitoas pré-púberes, alimentadas por 28 dias com dietas contendo zearalenona, foram avaliados. O delineamento experimental utilizado foi inteiramente ao acaso, com dois tratamentos, dieta controle (DC) e dieta controle + 2mg kg -1 de zearalenona (DZ), e seis repetições cada. Não houve diferença (P>0,05) entre os tratamentos para consumo médio diário de ração (1,24 x 1,19kg), ganho médio diário de peso (0,68 x 0,71kg), conversão alimentar (1,86 x 1,71) e peso vivo (PV); (30,9 x 30,4kg). A zearalenona não alterou (P>0,05) os pesos absoluto e relativo do coração (137 x 141g e 0,45 x 0,45% PV), fígado (699 x 699g e 2,31 x 2,26%PV), rins (47 x 49g e 0,15 x 0,16%PV) e baço (166 x 171g e 0,55 x 0,55%PV). Houve aumento (P<0,05) no comprimento (17 x 27cm) e no peso (23 x 157g e 0,07 x 0,51%PV) do trato reprodutivo das leitoas do grupo DZ. O volume vulvar ao final do período foi 820% maior (P<0,05) nos animais alimentados com zearalenona (941 x 8658mm 3 /kgPV 0,6 ). Os resultados indicam que em suínos a zearalenona e seus metabólitos possuem atividade estrogênica, mas não interferem no desempenho dos animais.

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“…However, globulin concentrations were not affected by the naturally contaminated diet. So, the reduction of serum protein concentrations may be due to the metabolic effect of ZEA, FUM and DON or its synergetic effect in the liver, which affects DNA and protein syntheses (Abid-Essefi et al, 2004;Speijers and Speijers, 2004). This variation in results of piglets fed naturally contaminated diet might be the result of differences in age of pigs, types of grain as mycotoxin sources, different doses of mycotoxins, and the presence of other mycotoxins as well as volume of blood sample collected (Dänicke et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Effects of feeding naturally contaminated diet with zearalenone, fumonisin and deoxynivalenol with or without yeast cell wall adsorbent on growth, vulva size and organ weights of gilts

Liu¹,

Yang²,

Yang³

et al. 2016

J. Anim. Feed Sci.

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Cytotoxicity, inhibition of DNA and protein syntheses and oxidative damage in cultured cells exposed to zearalenone

Cited by 228 publications

References 46 publications

Crocin and Quercetin protect HCT116 and HEK293 cells from Zearalenone-induced apoptosis by reducing endoplasmic reticulum stress

Crocin and Quercetin protect HCT116 and HEK293 cells from Zearalenone-induced apoptosis by reducing endoplasmic reticulum stress

Alimentação de leitoas pré-púberes com dietas contendo zearalenona

Effects of feeding naturally contaminated diet with zearalenone, fumonisin and deoxynivalenol with or without yeast cell wall adsorbent on growth, vulva size and organ weights of gilts

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