Cytotoxicity, Genotoxicity and Mutagenicity of Aluminum, Manganese and Lead in Meristematic Cells of Root Allium cepa

Franscisco, Luiza Flavia Veiga; Crispim, Bruno do Amaral; Viana, Lucilene Finoto; Nascimento, Hélina dos Santos; Raposo, Jorge Luiz; Barufatti, Alexéia

doi:10.17807/orbital.v10i1.1037

Cited by 10 publications

(8 citation statements)

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“…Therefore, studies that evaluate their cytotoxicity, genotoxicity, and mutagenicity in organisms are extremely important. Thus, bioassays with plants [ 38 , 39 , 40 ], animals [ 16 , 41 , 42 ], and human cells [ 5 , 16 , 43 , 44 , 45 ] are important tools for the assessment of the responses of organisms when exposed to metals.…”

Section: Discussionmentioning

confidence: 99%

Acute Toxic and Genotoxic Effects of Aluminum and Manganese Using In Vitro Models

Francisco

Baldivia

Crispim

et al. 2021

Toxics

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The objective of this study was to use the same concentrations of aluminum (Al) and manganese (Mn) detected previously in groundwater above those permitted by Brazilian law and assess their cytotoxic and genotoxic effects in hamster ovary cell lines and their mutagenic effects through the Salmonella microsome assay. Chinese hamster ovary (CHO) and CHO-XRS5 cells were treated with different concentrations of Al and Mn (0.2 to 2.0 mg/L and 0.1 to 3.0 mg/L, respectively). The Ames test was used to analyze the concentrations of Al and Mn ranging from 0.025 to 1.0 mg/L and 0.0125 to 1.5 mg/L, respectively. Both metals showed cytotoxic effects on both cell lines and two bacterial strains (TA98 and TA100). The genotoxic effects of the highest concentrations of Al and Mn in cell lines showed nuclear buds, micronuclei, and DNA damage; however, none of the concentrations showed a positive mutagenic response in the Ames test. This is one of the few studies to demonstrate the cytotoxic effects of Al and Mn through the Ames test. In addition, the metals caused genomic instability in cell lines. Therefore, this study may help hasten the review of established regulatory standards for human consumption of groundwater.

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Section: Discussionmentioning

confidence: 99%

Acute Toxic and Genotoxic Effects of Aluminum and Manganese Using In Vitro Models

Francisco

Baldivia

Crispim

et al. 2021

Toxics

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“…The inhibition of germination, as well as the decrease in MI values and increase in CAI, indicated the presence of substances in the water that promoted cytotoxicity and genotoxicity in A. cepa cells. The MI serves as an indicator for the analysis of the cytotoxic potential of various substances that may inhibit or increase cell proliferation (Leme and Marin-Morales 2009;Francisco et al 2018;). On the other hand, the chromosomal alterations measured by CAI indicated the presence of clastogenic and aneugenic agents in the environment, which allowed de ning the genotoxic effect (Athanásio et al 2014).…”

Section: Discussionmentioning

confidence: 99%

“…Positive control (PC) seeds were exposed to the herbicide tri uralin (0.84 ppm), and distilled water was used as a NC. Cytotoxicity, genotoxicity, and mutagenicity parameters, mitotic index (MI), chromosomal alteration index (CAI), mutagenicity index (MTI), and cell death rate (CDR), were calculated according to Leme and Marin-Morales (2009) and Francisco et al (2018).…”

Section: Land Usementioning

confidence: 99%

Environmental diagnosis of water quality in situ and ex situ in streams in Upper Paraná River Basin, Brazil

Meira

Silva

Viana

et al. 2021

Preprint

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The aim of this study was to (i) evaluate water quality using limnological parameters in three streams belonging to the Ivinhema River basin, (ii) investigate the composition and structure of the landscape around the locations sampled and their possible contribution to changes in water quality, (iii) investigate genotoxicity in fish in situ as environmental bioindicators, and (iv) evaluate the cytotoxic and genotoxic potential of water through bioassays in animal and plant models. A total of seven fish species were sampled in situ as bioindicators of environmental toxicogenetic damage: Astyanax fasciatus, Astyanax lacustris, Cyphocharax modestus, Moenkhausia forestii, Piabina argentea, Psellogrammus kennedyi, and Hypostomus ancistroides. Four types of nuclear and one cytoplasmic alteration were observed, with nuclear invagination being the most frequent. Animal bioassays showed five types of nuclear and one cytoplasmic alteration. In the plant bioassay, a reduction in germination and cell division was observed, indicating the cytotoxic and genotoxic effects of the water for Allium cepa cells. Thus, genotoxic alterations in fish and plant bioassays may reflect the different types of land use and cover around streams and the conversion of forest fragments into agricultural areas, mainly due to the expansion of sugarcane crops. The evaluation of the toxicogenetic status of the three streams is essential to emphasize the need to create projects to recover the native vegetation cover, which would improve the quality of the habitat and ensure the permanence of native species.

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“…Marin and Santos (2008) also expressed that Al caused toxicity in plants and reduced the density and the growth of roots. Francisco et al (2018) noticed a decrease in MI in meristematic cells of A. cepa exposed to Al. Fenech et al (1999) affirming that DNA damage was dependent on the rate of cell division.…”

Section: Groupsmentioning

confidence: 93%

Water quality assessment of the Demetrio stream: an affluent of the Gravataí River in the South of Brazil

et al. 2022

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The Gravataí River basin, one of the main water sources of the metropolitan region of Porto Alegre, is among the ten most polluted rivers in Brazil. Water quality is monitored only through physico-chemical and microbiological parameters in Brazil, and in this context, considering the importance of the use of biomarkers in complementing the analysis of water, the present study aimed to evaluate the environmental quality of the main affluent of the Gravataí River, Demetrio stream, through physico-chemical, microbiological, and cytogenotoxic criteria, at the stream source (P1), whereas samples P2 and P3 were obtained from the upstream near the area with the highest urban density and the downstream near the meeting point with the Gravataí River, respectively. The results for copper concentration and color classified the Demetrio stream as Class 4 in general, that is, the water is suitable only for navigation and to landscape harmony. The main genotoxic alterations (micronuclei and nuclear buds) were observed in P2, in which were obtained the highest levels of copper, in addition to iron and manganese. Anthropic interventions were observed in P1 and P2; however, due to its low metal concentration, P3, near the Gravataí River, manifested an improvement in environmental quality.

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Cytotoxicity, Genotoxicity and Mutagenicity of Aluminum, Manganese and Lead in Meristematic Cells of Root Allium cepa

Cited by 10 publications

References 0 publications

Acute Toxic and Genotoxic Effects of Aluminum and Manganese Using In Vitro Models

Acute Toxic and Genotoxic Effects of Aluminum and Manganese Using In Vitro Models

Environmental diagnosis of water quality in situ and ex situ in streams in Upper Paraná River Basin, Brazil

Water quality assessment of the Demetrio stream: an affluent of the Gravataí River in the South of Brazil

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