Cytotoxicity evaluation of three light-cured dentin adhesive materials on human gingival fibroblasts, ex vivo

Kierklo, Anna; Pawińska, Małgorzata; Tokajuk, Grażyna; Popławska, Bożena; Bielawska, Anna

doi:10.2478/v10039-012-0038-2

Cited by 14 publications

(13 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Overall, the Clearfil SE Bond showed severe cytotoxicity to all cell types, whereas Adper Single Bond 2 was reasonably well tolerated. These results are consistent with others described in the literature, although different experimental conditions were reported [ 14 , 18 , 28 , 29 ]. Demirci et al [ 14 ] found that Clearfil SE Bond leads to decreased cell viability in a concentration-dependent mode.…”

Section: Discussionsupporting

confidence: 93%

Laser Phototherapy Enhances Mesenchymal Stem Cells Survival in Response to the Dental Adhesives

Diniz

Matos

Marques

2015

The Scientific World Journal

View full text Add to dashboard Cite

Background. We investigated the influence of laser phototherapy (LPT) on the survival of human mesenchymal stem cells (MSCs) submitted to substances leached from dental adhesives. Method. MSCs were isolated and characterized. Oral mucosa fibroblasts and osteoblast-like cells were used as comparative controls. Cultured medium conditioned with two adhesive systems was applied to the cultures. Cell monolayers were exposed or not to LPT. Laser irradiations were performed using a red laser (GaAlAs, 780 nm, 0.04 cm2, 40 mW, 1 W/cm2, 0.4 J, 10 seconds, 1 point, 10 J/cm2). After 24 h, cell viability was assessed by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide reduction assay. Data were statistically compared by ANOVA followed by Tukey's test (P < 0.05). Results. Different cell types showed different viabilities in response to the same materials. Substances leached from adhesives were less cytotoxic to MSCs than to other cell types. Substances leached from Clearfil SE Bond were highly cytotoxic to all cell types tested, except to the MSCs when applied polymerized and in association with LPT. LPT was unable to significantly increase the cell viability of fibroblasts and osteoblast-like cells submitted to the dental adhesives. Conclusion. LPT enhances mesenchymal stem cells survival in response to substances leached from dental adhesives.

show abstract

Section: Discussionsupporting

confidence: 93%

Laser Phototherapy Enhances Mesenchymal Stem Cells Survival in Response to the Dental Adhesives

Diniz

Matos

Marques

2015

The Scientific World Journal

View full text Add to dashboard Cite

show abstract

“…Monomers such as TEGDMA, HEMA, and 10-MDP appeared to contribute to the inflammation and inhibition of odontoblastic differentiation also by inducing oxidative stress via the production of reactive oxygen species and reduction of intracellular glutathione levels [ 17 , 19 , 29 , 30 ]. Moreover, the combination of Bis-GMA, dimethacrylate, and/or polyalcene acids in the bonding system may play a role in the apparent cytotoxicity of this product and may suppress the healing capacity of the pulp tissue [ 7 , 31 ]. In a study by Lee et al [ 32 ], the absence of complete bridging in a group treated with TheraCal was attributed to the lower biocompatibility of the material, which caused a higher degree of inflammation due to the acrylic monomer Bis-GMA present in the material.…”

Section: Discussionmentioning

confidence: 99%

Clinical and Histological Evaluation of Direct Pulp Capping on Human Pulp Tissue Using a Dentin Adhesive System

Nowicka

Łagocka

Lipski

et al. 2016

BioMed Research International

View full text Add to dashboard Cite

Objective. This study presents a clinical and histological evaluation of human pulp tissue responses after direct capping using a new dentin adhesive system. Methods. Twenty-eight caries-free third molar teeth scheduled for extraction were evaluated. The pulps of 22 teeth were mechanically exposed and randomly assigned to 1 of 2 groups: Single Bond Universal or calcium hydroxide. Another group of 6 teeth acted as the intact control group. The periapical response was assayed, and a clinical examination was performed. The teeth were extracted after 6 weeks, and a histological analysis was performed. The pulp status was assessed, and the thickness of the dentin bridge was measured and categorized using a histological scoring system. Results. The clinical phase was asymptomatic for Single Bond Universal patients. Patients in the calcium hydroxide group reported mild symptoms of pain, although the histological examination revealed that dentin bridges with or without limited pulpitis had begun forming in each tooth. The universal adhesive system exhibited nonsignificantly increased histological signs of pulpitis (P > 0.05) and a significantly weaker thin mineralized tissue layer (P < 0.001) compared with the calcium hydroxide group. Conclusion. The results suggest that Single Bond Universal is inappropriate for human pulp capping; however, further long-term studies are needed to determine the biocompatibility of this agent.

show abstract

“…This test has been used by many authors in order to evaluate the cytotoxicity of various materials for application in Dentistry [29][30][31][32]. The MTT assay is reported to have several advantages including the fast analyses, measurements and management of many samples [28].…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity of non-vital dental bleaching agents in human gingival fibroblasts

Fernandes

Marques

Camargo

et al. 2013

BDS

View full text Add to dashboard Cite

Purpose: The aim of this study was to compare several non-vital dental bleaching agents for their in vitro cytotoxicity to human gingival fibroblasts primary cell line.Methods: The cells were cultivated in DMEM and were seed in plates of 96 wells; then, it was exposed to the conditioned medium according to the experimental groups (n=12): G1- SP (sodium perborate) + distilled water; G2- SP + 20% CP (carbamide peroxide); G3- 20% CP; G4- SP + 35% HP (hydrogen peroxide); G5- 35% HP. In the control group (n=12), corresponded to the curve of cell growth and viability, the cells did not receive any treatment. Cell viability was measured photometrically using a MTT assay after a 24h and 48h of exposure period. Data were submitted to ANOVA and Tukey’s tests.Results: All the experimental groups presented high cytotoxicity statically in comparison to the control group. The rank of the most to the least toxic material after 24 hours was: SP + DW > 35% PH > PS + 20% PC > PS + 35% PH > 20% PC; and after 48 hours was: SP + DW > PS + 20% PC > 35% PH > PS + 35% PH > 20% PC. All the bleaching agents had presented cytotoxicity effects, reducing significantly the cell viability, however, in the conditions that the study was conducted the association of sodium perborate with distilled water was the most toxic bleaching agent.

show abstract

Cytotoxicity evaluation of three light-cured dentin adhesive materials on human gingival fibroblasts, ex vivo

Cited by 14 publications

References 24 publications

Laser Phototherapy Enhances Mesenchymal Stem Cells Survival in Response to the Dental Adhesives

Laser Phototherapy Enhances Mesenchymal Stem Cells Survival in Response to the Dental Adhesives

Clinical and Histological Evaluation of Direct Pulp Capping on Human Pulp Tissue Using a Dentin Adhesive System

Cytotoxicity of non-vital dental bleaching agents in human gingival fibroblasts

Contact Info

Product

Resources

About