Cytotoxicity evaluation of four endodontic sealers

Silva, Paulo Tadeu da; Pappen, Fernanda Geraldo; Souza, Erick Miranda; Dias, João Eduardo; Filho, Idomeo Bonetti; Carlos, Iracilda Z.; Leonardo, Renato de Toledo

doi:10.1590/s0103-64402008000300010

Cited by 22 publications

(21 citation statements)

References 18 publications

(19 reference statements)

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“…The findings of the present study agree with several previous studies that showed that RoekoSeal was only little cytotoxic or completely non-cytotoxic even in fresh conditions 9,11,12,21 . The relatively severe and mild cytotoxic responses exhibited by the other sealers in fresh conditions, confirm the results reported in previous studies 2,7,9,11,12,18,22,23 . This could be attributed to the release of small amounts of toxic substances present in the sealers.…”

Section: Discussionsupporting

confidence: 90%

Long-term cytotoxic effects of contemporary root canal sealers

2013

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Objectives:The aim of the present study was to investigate the effects of root canal sealers on the cytotoxicity of 3T3 fibroblasts during a period of 5 weeks.Material and Methods:Fibroblasts (3T3, 1x105 cells per well) were incubated with elutes of fresh specimens from eight root canal sealers (AH Plus, Epiphany, Endomethasone N, EndoReZ, MTA Fillapex, Pulp Canal Sealer EWT, RoekoSeal and Sealapex) and with elutes of the same specimens for 5 succeeding weeks after immersing in simulated body fluid. The cytotoxicity of all root canal sealers was determined using the MTT assay. Data were analyzed using ANOVA and Tukey's test.Results:RoekoSeal was the only sealer that did not show any cytotoxic effects (p<0.05). All the other tested sealers exhibited severe toxicity initially (week 0). MTA Fillapex remained moderately cytotoxic after the end of experimental period. Toxicity of the other tested sealers decreased gradually over time. The evaluated root canal sealers presented varying degrees of cytotoxicity, mainly in fresh mode.Conclusions:RoekoSeal had no cytotoxic effect both freshly mixed and in the other tested time points. MTA Fillapex was associated with significantly less cell viability when compared to the other tested root canal sealers.

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Section: Discussionsupporting

confidence: 90%

Long-term cytotoxic effects of contemporary root canal sealers

2013

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“…Although in our daily life, the clinical application of eugenol is still limited to dentistry (Zinc oxide –eugenol fillings), it is important to note that the chemosensitivity of MCF-7 cells to eugenol occurs at lower concentrations than those previously reported (1–4 mM) in non-cancer cells6061 and those commonly used in dental practice15161741. Elucidation of the key players underlying the sensitivity to eugenol and mechanistic differences between normal and cancer cells to eugenol requires further in vivo and in vitro investigations.…”

Section: Discussionmentioning

confidence: 97%

“…However, despite the reported safety, concentration-dependent adverse side effects of eugenol have been noted111213. Reduced healing process, cytotoxicity and necrosis were observed in eugenol (0.1–1 mM) treated macrophages1415, human periodontal ligament cells16, osteoblasts17, and fibroblasts18.…”

mentioning

confidence: 99%

Chemosensitivity of MCF-7 cells to eugenol: release of cytochrome-c and lactate dehydrogenase

Wafai

El-Rabih

Katerji

et al. 2017

Sci Rep

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Phytochemicals have been extensively researched for their potential anticancer effects. In previous study, direct exposure of rat liver mitochondria to eugenol main ingredient of clove, uncoupled mitochondria and increased F0F1ATPase activity. In the present study, we further investigated the effects of eugenol on MCF-7 cells in culture. Eugenol demonstrated: a dose-dependent decrease in viability (MTT assay), and proliferation (real time cell analysis) of MCF-7 cells, (EC50: 0.9 mM); an increase in reactive oxygen species; a decrease in ATP level and mitochondrial membrane potential (MitoPT JC-1 assay); and a release of cytochrome-c and lactate dehydrogenase (Cytotoxicity Detection Kit PLUS) into culture media at eugenol concentration >EC50. Pretreatment with the antioxidants Trolox and N-acetyl cysteine partially restored cell viability and decreased ROS, with Trolox being more potent. Expression levels of both anti- and pro-apoptotic markers (Bcl-2 and Bax, respectively) decreased with increasing eugenol concentration, with no variation in their relative ratios. Eugenol-treated MCF-7 cells overexpressing Bcl-2 exhibited results similar to those of MCF-7. Our findings indicate that eugenol toxicity is non-apoptotic Bcl-2 independent, affecting mitochondrial function and plasma membrane integrity with no effect on migration or invasion. We report here the chemo-sensitivity of MCF-7 cells to eugenol, a phytochemical with anticancer potential.

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“…2 h later, non-adherent cells were discarded. The remaining adherent cells were maintained in culture for 24 h until they were used for experiments [53].…”

Section: Methodsmentioning

confidence: 99%

Effects of Aging on Endotoxin Tolerance Induced by Lipopolysaccharides Derived from Porphyromonas gingivalis and Escherichia coli

Sun

Yang

et al. 2012

PLoS ONE

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BackgroundPeriodontitis is a bacterially induced chronic inflammatory disease. Exposure of the host to periodontal pathogens and their virulence factors induces a state of hyporesponsiveness to subsequent stimulations, termed endotoxin tolerance. Aging has a profound effect on immune response to bacteria challenge. The aim of this study was to explore the effects of aging on endotoxin tolerance induced by Porphyromonas gingivalis (P. gingivalis) lipopolysaccharide (LPS) and Escherichia coli (E. coli) LPS in murine peritoneal macrophages.Methodology/Principal FindingsWe studied the cytokine production (TNF-αand IL-10) and Toll-like receptor 2, 4 (TLR2, 4) gene and protein expressions in peritoneal macrophages from young (2-month-old) and middle-aged (12-month-old) ICR mice following single or repeated P. gingivalis LPS or E. coli LPS stimulation. Pretreatment of peritoneal macrophages with P. gingivalis LPS or E. coli LPS resulted in a reduction in TNF-α production and an increase in IL-10 production upon secondary stimulation (p<0.05), and the markedly lower levels of TNF-α and higher levels of IL-10 were observed in macrophages from young mice compared with those from middle-aged mice (p<0.05). In addition, LPS restimulations also led to the significantly lower expression levels of TLR2, 4 mRNA and protein in macrophages from young mice (p<0.05).Conclusions/SignificanceRepeated LPS stimulations triggered endotoxin tolerance in peritoneal macrophages and the ability to develop tolerance in young mice was more excellent. The impaired ability to develop endotoxin tolerance resulted from aging might be related to TLR2, 4 and might lead to the incontrollable periodontal inflammation in older adults.

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Cytotoxicity evaluation of four endodontic sealers

Cited by 22 publications

References 18 publications

Long-term cytotoxic effects of contemporary root canal sealers

Long-term cytotoxic effects of contemporary root canal sealers

Chemosensitivity of MCF-7 cells to eugenol: release of cytochrome-c and lactate dehydrogenase

Effects of Aging on Endotoxin Tolerance Induced by Lipopolysaccharides Derived from Porphyromonas gingivalis and Escherichia coli

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