Cytotoxicity effects of amiodarone on cultured cells

Golli-Bennour, Emna El; Bouslimi, Amel; Zouaoui, Olfa; Nouira, S.; Achour, A.; Bacha, Hassen

doi:10.1016/j.etp.2010.10.008

Cited by 33 publications

(21 citation statements)

References 38 publications

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“…An increase in hepatic carbohydrates, proteins and DNA was observed in rats given and AlCl 3 plus grapefruit. These results are in line with the report of [36] and [37] who reported that grapefruit juice restores the renal contents of carbohydrates and proteins to normal contents. [13] reported that rats were given grapefruit juice prior to treatment with aflatoxin-B 1 resulted in a noticeable reduction of DNA damage in the liver.…”

Section: Discussionsupporting

confidence: 83%

Effect of Grapefruit Juice on Aluminum-Induced Hepatotoxicity in Albino Rats: Histological, Ultrastructural and Histochemical Assessment

Al-Amoudi¹

2017

ABB

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The present work studied the effect of grapefruit juice (Citrus) on the hepatotoxicity of AlCl 3 . Four groups of adult male albino rats were used. Group I contained 10 rats and remained as a control group; Group II: 10 rats were included and orally administered with grapefruit juice at a dose level of 27 ml/kg of body weight per day for 6 weeks; Group III: 10 rats were included and AlCl 3 was administered orally at a dose level of 100 mg/kg of body weight per day for 6 weeks; Group IV: animals of this group (10 rats) were given a combination of AlCl 3 and grapefruit juice per day for 6 weeks. At the end of the sixth week, the liver was processed for histological and ultrastructure studies. In addition, ALT and AST were determined in blood sera. The results showed that AlCl 3 induced several histological alterations in the liver, including congestion of blood vessels, leucocytic infiltration, cytoplasmic vacuolation of hepatocytes and fatty infiltrations. Ultrastructural changes include the degeneration of mitochondria, RER swelling and pyknosis of the nuclei with an increase in fat droplets and lysosomes. The biochemical results showed an increase in ALT and AST activities in the AlCl 3 group. However, the group of rats treated with AlCl 3 and grapefruit juice improved the histological and ultrastructural changes, while the levels of ALT and AST were reduced. It was concluded in the results that grapefruit juice has a protective effect against hepatotoxicity of AlCl 3 that can be attributed to the antioxidant properties of its components.

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Section: Discussionsupporting

confidence: 83%

Effect of Grapefruit Juice on Aluminum-Induced Hepatotoxicity in Albino Rats: Histological, Ultrastructural and Histochemical Assessment

Al-Amoudi¹

2017

ABB

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“…Eahy.926 cells are vascular endothelial cells and are used as a cellular model of the vascular wall. Eahy.926 cells are used to study the toxicity of nanomaterials on the vascular endothelium [32,33]. The reason for the choice of these two cells lies in the application of these nanomaterials in drug delivery.…”

Section: Discussionmentioning

confidence: 99%

Nanotoxicity comparison of four amphiphilic polymeric micelles with similar hydrophilic or hydrophobic structure

Zhao

Wang

et al. 2013

Part Fibre Toxicol

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BackgroundNanocarriers represent an attractive means of drug delivery, but their biosafety must be established before their use in clinical research.ObjectivesFour kinds of amphiphilic polymeric (PEG-PG-PCL, PEEP-PCL, PEG-PCL and PEG-DSPE) micelles with similar hydrophilic or hydrophobic structure were prepared and their in vitro and in vivo safety were evaluated and compared.MethodsIn vitro nanotoxicity evaluations included assessments of cell morphology, cell volume, inflammatory effects, cytotoxicity, apoptosis and membrane fluidity. An umbilical vein cell line (Eahy.926) and a kind of macrophages (J774.A1) were used as cell models considering that intravenous route is dominant for micelle delivery systems. In vivo analyses included complete blood count, lymphocyte subset analysis, detection of plasma inflammatory factors and histological observations of major organs after intravenous administration to KM mice.ResultsAll the micelles enhanced inflammatory molecules in J774.A1 cells, likely resulting from the increased ROS levels. PEG-PG-PCL and PEEP-PCL micelles were found to increase the J774.A1 cell volume. This likely correlated with the size of PEG-PG-PCL micelles and the polyphosphoester structure in PEEP-PCL. PEG-DSPE micelles inhibited the growth of Eahy.926 cells via inducing apoptosis. This might relate to the structure of DSPE, which is a type of phospholipid and has good affinity with cell membrane. No evidence was found for cell membrane changes after treatment with these micelles for 24 h. In the in vivo study, during 8 days of 4 time injection, each of the four nanocarriers altered the hematic phase differently without changes in inflammatory factors or pathological changes in target organs.ConclusionsThese results demonstrate that the micelles investigated exhibit diverse nanotoxicity correlated with their structures, their biosafety is different in different cell model, and there is no in vitro and in vivo correlation found. We believe that this study will certainly provide more scientific understandings on the nanotoxicity of amphiphilic polymeric micelles.

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“…Choi et al (2002) demonstrated that L-132 human lung cell line treated with amiodarone exhibited several features of apoptosis and increase in the messenger RNA levels of Bax and caspase 3. It was reported that the predominant molecular mechanism of amiodarone-induced toxicity is the trigger caused by oxidative damage due to production of reactive oxygen species (Golli-Bennour et al, 2010). Vereckei et al (1993) mentioned that amiodarone generated free radicals in vitro by chemiluminometric method and caused a significant increase of nicotinamide adenine dinucleotide phosphate, and ferrous ion induced lipid peroxidation in the liver microsomal fraction.…”

Section: Discussionmentioning

confidence: 99%

Effect of grapefruit juice on amiodarone induced nephrotoxicity in albino rats

Sakr

El-Gamal

2013

Toxicol Ind Health

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Amiodarone is a potent antiarrhythmic drug that is used to treat ventricular and supraventricular tachyarrhythmias. The present work studied the effect of amiodarone on the kidney of albino rats and the possible ameliorative role of grapefruit juice. Administration of amiodarone by gastric intubation (18 mg/kg body weight (b.w.), daily for 5 weeks) caused many histological alterations including intertubular leucocytic infiltrations, degeneration of the renal tubules, and atrophy of the glomeruli. Amiodarone caused marked elevation in serum creatinine and blood urea nitrogen. Histochemical examination of the renal tubules revealed depletion of glycogen and total proteins. Besides, animals administered with amiodarone showed an increase of apoptotic bands as detected by gel electrophoresis. Treating animals with amiodarone and grapefruit juice (27 ml/kg b.w.) caused an improvement in histological and histochemical appearance of the kidney together with decrease of serum creatinine and blood urea nitrogen. Moreover, the apoptosis was decreased. It is concluded from the obtained results that grapefruit juice ameliorates the nephrotoxicity of amiodarone in albino rats and this may be due to the potent antioxidant effects of its components.

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Cytotoxicity effects of amiodarone on cultured cells

Cited by 33 publications

References 38 publications

Effect of Grapefruit Juice on Aluminum-Induced Hepatotoxicity in Albino Rats: Histological, Ultrastructural and Histochemical Assessment

Effect of Grapefruit Juice on Aluminum-Induced Hepatotoxicity in Albino Rats: Histological, Ultrastructural and Histochemical Assessment

Nanotoxicity comparison of four amphiphilic polymeric micelles with similar hydrophilic or hydrophobic structure

Effect of grapefruit juice on amiodarone induced nephrotoxicity in albino rats

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