Cytotoxicity Effect of Solid Lipid Nanoparticles on Human Breast Cancer Cell Lines

Abbasalipourkabir, Roghayeh; Salehzadeh, Aref; Abdullah, Rasedee

doi:10.3923/biotech.2011.528.533

Cited by 34 publications

(18 citation statements)

References 6 publications

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“…These results demonstrate that NLS is a biocompatible formulation that is safe for use in eukaryotic cells; natural lipids are well tolerated by living organisms (Huang, 2008; Abbasalipourkabirreh et al, 2011; Oliveira et al, 2015). The SI is calculated by dividing the IC 50 value by the MIC value.…”

Section: Discussionmentioning

confidence: 71%

Alkyl Protocatechuate-Loaded Nanostructured Lipid Systems as a Treatment Strategy for Paracoccidioides brasiliensis and Paracoccidioides lutzii In Vitro

et al. 2017

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Dodecyl protocatechuate (dodecyl) is a derivative of protocatechuic acid (3,4-dihydroxybenzoic acid) that possesses anti-oxidant and antifungal properties. Nanostructured lipid systems (NLS) can potentiate the action of many antifungal agents, reducing the required dose and side effects by improving their activity. This work aimed to evaluate dodecyl protocatechuate loaded into a NLS (NLS+dodecyl) as a strategy for the treatment of Paracoccidioides brasiliensis and P. lutzii in vitro. Antifungal activity against P. brasiliensis and P. lutzii was evaluated using the microdilution technique. NLS+dodecyl showed high antifungal activity with a minimum inhibitory concentration ranging from 0.06 to 0.03 μg/mL; 4- to 16-fold higher than that of free dodecyl. NLS+dodecyl was able to inhibit fungal adhesion of the extracellular artificial matrix proteins (laminin and fibronectin), resulting in 82.4 and 81% inhibition, respectively, an increase of 8–17% compared with free dodecyl. These findings corroborate previous results demonstrating 65 and 74% inhibition of fungal adhesion in pulmonary fibroblast cells by dodecyl and NLS+dodecyl, respectively, representing a 9% increase in inhibition for NLS+dodecyl. Subsequently, cytotoxicity was evaluated using the 0.4% sulforhodamine B assay. NLS+dodecyl did not exhibit cytotoxicity in MRC5 (human pneumocyte) and HepG2 (human hepatic carcinoma) cells, thus increasing the selectivity index for NLS+dodecyl. In addition, cytotoxicity was evaluated in vivo using the Caenorhabditis elegans model; neither dodecyl nor NLS+dodecyl exhibited any toxic effects. Taken together, these results suggest that NLS can be used as a strategy to improve the activity of dodecyl against P. brasiliensis and P. lutzii because it improves antifungal activity, increases the inhibition of fungal adhesion in lung cells and the extracellular matrix in vitro, and does not exhibit any toxicity both in vitro and in vivo.

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Section: Discussionmentioning

confidence: 71%

Alkyl Protocatechuate-Loaded Nanostructured Lipid Systems as a Treatment Strategy for Paracoccidioides brasiliensis and Paracoccidioides lutzii In Vitro

et al. 2017

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“…>80% of cells remain viable), which is comparable to or better than more widely studied drug nanocarriers at the same concentrations. 57,58 More extensive studies of biological transport and toxicity are ongoing.…”

Section: Adaptability Of Double Nanoemulsion Nanogel Synthesis To Othmentioning

confidence: 99%

Synthesis of Oil-Laden Poly(ethylene glycol) Diacrylate Hydrogel Nanocapsules from Double Nanoemulsions

et al. 2017

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Multiple emulsions have received great interest due to their ability to be used as templates for the production of multi-compartment particles for a variety of applications.However, scaling these complex droplets to nanoscale dimensions has been a challenge due to limitations on their fabrication methods. Here, we report the development of oil-in-water-in-oil (O1/W/O2) double nanoemulsions via a two-step high-energy method, and their use as templates for complex nanogels comprised of inner oil droplets encapsulated within a hydrogel matrix.Using a combination of characterization methods, we determine how the properties of the nanogels are controlled by the size, stability, internal morphology, and chemical composition of the nanoemulsion templates from which they are formed. This allows for identification of 2 compositional and emulsification parameters that can be used to optimize the size and oil encapsulation efficiency of the nanogels. Our templating method produces oil-laden nanogels with high oil encapsulation efficiencies and average diameters of 200-300 nm. In addition, we demonstrate the versatility of the system by varying the types of inner oil, the hydrogel chemistry, the amount of inner oil, and the hydrogel network crosslink density. These non-toxic oil-laden nanogels have potential applications in food, pharmaceutical, and cosmetic formulations.

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“…Several studies have shown that chitosan nanoparticles (ChNPs) could exert a high cytotoxicity against EAC cell line, this is due to their ability to adhere to the cell membrane and degrade products in the cell culture medium or inside the cells [33,34]. ChNPs can be first adsorbed onto the negatively charged tumor cell membrane by electron interaction, then exhibit antitumor effects by damaging membrane and disrupting organelle, and finally lead to cell death with the structure breakdown [35].The reactive hydroxyl and amine groups of chitosan facilitate the adherence to negatively charged surfaces, thereby enhancing cellular transport.…”

Section: Discussionmentioning

confidence: 99%

Antioxidative stress of Chitosan-Gallium Nanoparticles and low doses of γ-irradiation on mice bearing Ehrlich carcinoma

Mohammad¹,

Ahmed²,

Kandil³

et al. 2019

IJASTR

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Guided treatments with nanoparticles and low doses of radiation are a new approach in cancer therapy. This study evaluated the ability of Chitosan-Gallium nanoparticles (Ch.GaNPs) and low dose ofγ-radiation to suppressoxidative stressof female mice bearing solid Ehrlich carcinoma (EC)in the neck region. Begins from 7 th day of EC cells inoculation Ch.GaNPs(0.5 mg/kg body weight) were orally administered via gavages for a period of 15 consecutive days and Whole-body γ -irradiation was carried out at a single dose of 0.25Gy/twice a week for 2 weeks. Tumor size was monitored. Oxidative stress markers were assessed, histopathological changes in tumor and liver tissues were studied. In addition, the angiogenic markers concentrations were evaluated.Treatment of EC-bearing mice with Ch.GaNPs and/or low doseof γ -radiation exposure significantly reduced tumor volume, increased lipid peroxidation and decreased glutathione content as well as glutathione peroxidase activity intumor and liver tissues. Moreover, the dual treatment significantly reducedserumVEGF, PDGF and TNF-α levels, and significantly elevated Cas-3 activity. These results were well appreciated with the histopathologicalfindings in the tumor and liver tissue. In conclusion, the combined treatment of Ch.GaNPs and low dose ofγ-radiation have a role in suppressing oxidative stress of female mice bearing solid Ehrlich carcinoma .

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Cytotoxicity Effect of Solid Lipid Nanoparticles on Human Breast Cancer Cell Lines

Cited by 34 publications

References 6 publications

Alkyl Protocatechuate-Loaded Nanostructured Lipid Systems as a Treatment Strategy for Paracoccidioides brasiliensis and Paracoccidioides lutzii In Vitro

Alkyl Protocatechuate-Loaded Nanostructured Lipid Systems as a Treatment Strategy for Paracoccidioides brasiliensis and Paracoccidioides lutzii In Vitro

Synthesis of Oil-Laden Poly(ethylene glycol) Diacrylate Hydrogel Nanocapsules from Double Nanoemulsions

Antioxidative stress of Chitosan-Gallium Nanoparticles and low doses of γ-irradiation on mice bearing Ehrlich carcinoma

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