Cytotoxicity Effect and Morphological Study of Different Duku(Lansium domesticum corr.) Extract towards Human Colorectal Adenocarcinoma Cells Line (HT-29)

Rohin, Mohd Adzim Khalili; Noratiqah, Jumli Mimie; Ridzwan, Norhaslinda; Norhayati, Abd Hadi; Amin, Baig Atif; Arshad, Roslan; Zubaidi, A. L. Ahmad

doi:10.5530/pj.2017.6.119

Cited by 19 publications

(15 citation statements)

References 9 publications

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“…10 Exponentially growing cells were harvested, enumerated using the hemocytometer and diluted with a medium. A cell volume with a concentration of 1.0 x 10 4 cells/mL was prepared and was plated (100 μL/well) into 96-well plates (NUNCTM, Denmark).…”

Section: Mtt Assays and Determination Of Ic 50mentioning

confidence: 99%

Effect of Gynura procumbens Extracts on Anti-Proliferative Activity and its Associated Morphological Changes of Human Glioblastoma multiforme Cell Line (U-87)

Rohin¹,

Jumli²,

Ridzwan³

et al. 2018

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Objective: Gynura procumbens (Lour.) Merr is a well-known traditional herb and is widely used for traditional medicine by human. Previous clinical studies have shown the benefit effect of this leaves with inflammation, high blood pressure and others. This study aims to investigate the effect of methanol, ethanol, and ethyl acetate extracts of Gynura procumbens on U-87 cell line, human Glioblastoma multiforme cell line. Methods: In the present study, effect of methanol, ethanol and ethyl acetate extracts on U-87 cell line was determined by MTT-based anti-proliferative assay and any significant changes to the cells towards apoptotic changes was observed under a light microscope. Results: The ethanol and methanol extracts of Gynura procumbens was found to have high anti-proliferative effect on U-87 cell line with IC 50 less than 20µg/ml. By comparing IC 50 value, ethanol and methanol extracts of Gynura procumbens had high potential for anti-proliferative effect on U-87 cell line. Therefore, ethanol and methanol extracts can be a potential anti-proliferative effect on U-87 cell line. Conclusion: To the best of our knowledge, this is the first study of exploring the effect of Gynura procumbens extracts using three different polarity (methanol, ethanol, ethyl acetate) effect on U-87 cell line, Human Glioblastoma multiforme cells.

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Section: Mtt Assays and Determination Of Ic 50mentioning

confidence: 99%

Effect of Gynura procumbens Extracts on Anti-Proliferative Activity and its Associated Morphological Changes of Human Glioblastoma multiforme Cell Line (U-87)

Rohin¹,

Jumli²,

Ridzwan³

et al. 2018

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“…The methods used previously by Khalili et al [16] were adopted for this study with slight modification. A total of 10 mg of different singgang extracts were dissolved in 1 mL of DMSO to prepare a stock solution of 10 mg/mL.…”

Section: Preparation Of Singgang Extractsmentioning

confidence: 99%

Fish Singgang Extracts as a Potential Anti-Proliferative against Colon Cancer Cell Lines (HT-29, HCT-116, CT-26)

Jamain¹,

Anwar²,

Ridzwan³

et al. 2021

JPRI

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Aim: To investigate anti-proliferative effect of three types of Terengganu singgang extracts on colon cancer cell lines (HT-29, HCT-116, CT-26). Study Design: Experimental study. Place and Duration of Study: Central Laboratory, Tissue Culture Laboratory, Universiti Sultan Zainal Abidin, Terengganu between April 2019 and July 2019. Methodology: Samples comprised three types of singgang dish, which were prepared, cooked, and then extracted with distilled water and ethanol (EtOH) in different strengths, 50%, 70%, and 100%.These singgang samples were chub mackerel (ST), Indian mackerel (SK),and a control sample with no fish (SC). Extracts were analyzed for their anti-proliferative effect by MTT-based assay. Then, the morphological of cell apoptotic changes was observed using light inverted microscope. Results: Experimental assays showed that the SC sample extracted in 100% EtOH produced the highest yield (3.7%).The extract of ST in aqueous (0.27 (0.11)) yielded the most cytotoxic value, followed by extract SK in 100% EtOH (0.28 (0.10)) and extract SC in 50% EtOH (0.20 (0.08)). Then, the anti-proliferative effect was confirmed with morphological changes of cell which were characterized by cell shrinkage, membrane blebbing, and fragmentation of apoptotic bodies after 24, 48 and 72 hours of treatment. Conclusion: In conclusion, the ST extract showed the best anti-proliferative effect.

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“…The methods used previously by Khalili et al [15] were adopted for this study with modifications. A total of 1 mg of different polarity solvents of P. minus extracts were dissolved in 1 mL of DMSO to prepare a stock solution of 1 mg/mL.…”

Section: Preparation Of P Minus Extractsmentioning

confidence: 99%

Different Polarity Extracts of Polygonum minus towards Cytotoxic Activities against Colon Cancer Cell Lines (HT-29, HCT-116, CT-26)

Rohin

Hadi

Ridzwan

2020

JPRI

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Aims: To investigate the cytotoxic properties of different polarity solvents of Polygonum minus extracts towards colon cancer cell lines, HT-29, HCT-116 and CT-26. Study Design: Experimental study. Place and Duration of Study: Central Laboratory, Tissue Culture Laboratory, Universiti Sultan Zainal Abidin, Terengganu between September 2019 until December 2019. Methodology: The different polarity solvents of P. minus extracts had been led to tetrazolium salt reduction (MTT) assay and an inhibition concentration of 50 (IC50) value for their cytotoxic potential against colon cancer cells. Then, cell morphology observation and fluorescence double staining of treatment cells were determined using a light inverted microscope and acridine orange/propidium iodide staining. Results: The results indicated that an extraction yields aligned from 0.01% for acetone and ethyl acetate to 0.45% for aqueous solution with decreasing order of aqueous solution > 70% aqueous ethanol > 50% aqueous ethanol > methanol > ethanol > acetone and ethyl acetate. Meanwhile, the ethyl acetate extract showed a higher cytotoxic effect at IC50 values of 7.00 ± 0.06 µg/mL and 7.00 ± 0.30 µg/mL towards the HCT-116 and CT-26 cells; and 50% aqueous ethanol towards HT-29 cells (24.00 ± 0.01 µg/mL). The different solvent extracts of P. minus induced cytotoxic effects on the treated cell lines by altering their normal cell morphology and cell membrane integrity (except for acetone extract). Conclusion: Therefore, the use of different polarity solvent extracts of P. minus as an anti-cancer agent is promising more on ethyl acetate and warrants further investigation.

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Cytotoxicity Effect and Morphological Study of Different Duku(Lansium domesticum corr.) Extract towards Human Colorectal Adenocarcinoma Cells Line (HT-29)

Cited by 19 publications

References 9 publications

Effect of Gynura procumbens Extracts on Anti-Proliferative Activity and its Associated Morphological Changes of Human Glioblastoma multiforme Cell Line (U-87)

Effect of Gynura procumbens Extracts on Anti-Proliferative Activity and its Associated Morphological Changes of Human Glioblastoma multiforme Cell Line (U-87)

Fish Singgang Extracts as a Potential Anti-Proliferative against Colon Cancer Cell Lines (HT-29, HCT-116, CT-26)

Different Polarity Extracts of Polygonum minus towards Cytotoxic Activities against Colon Cancer Cell Lines (HT-29, HCT-116, CT-26)

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