Cytotoxicity Brine Shrimp Activity of Leptadenia Hastata (PER) Decne Leaves, Stem-Bark and Root Extract

Abstract

Objective: The present study was conducted to test for in vivo Brine Shrimp Cytotocity activity of Leptadenia hastata leaves, stem-bark and root extracts after successive maceration in five solvents (n-hexane, dichloromethane (DCM) ethyl acetate chloroform and methanol) and correlate cytotoxicity results with known pharmacological activities of the plant.Methods: Cytotoxicity was evaluated in terms of LC50 (lethality concentration), 10 nauplii were added into three replicates of each concentration of the plant extracts, and after 24 h the surviving brine shrimp larvae were counted, and LC50was assessed.Results: Potent cytotoxicity was found for both the leaves, stem-bark and root extracts of Leptadenia hastata, results showed a concentration dependent increment in mortality rate of the brine shrimp nauplii and the n-hexane dichloromethane ethyl acetate, chloroform and methanol fractions of the leaves, stem-bark and root extracts were potent against the brine shrimp, with chloroform Leaf extract having the highest 3460.00 and Methanol as the lowest LC50 with 651.292. Conclusion:The result indicated that bioactive components are present in these plants that could be accounted for its pharmacological effects. Thus, the results support the uses of these plant species in traditional medicine.

Assessment of Cytotoxic, Antioxidant, Thrombolytic, Anti Inflammatory and Antimicrobial Activity of Curcuma longa Linn, Cissus quadrangularis and Boerhaavia diffusa Herbal Mixture - An In vitro Study

“…The brine shrimp test is a rapid, economical and easy bioassay for determining the lethality of plant extracts, which correlates relatively well with cytotoxic and anti-tumor properties in most cases [23]. The majority of the time, a desired biological response is due to a combination of bioactive plant components, rather than a single component [24].…”

Toksisitas Akut Ekstrak Etanol Batang Rumput Knop (Hyptis capitata Jacq.) dengan Metode Brine Shrimp Lethality Test (BSLT)

“…Nilai LC 50 880,579 µg/ml menunjukkan bahwa ekstrak etanol batang rumput Knop bersifat toksik terhadap larva Artemia salina (L.). Namun tingkat toksisitas tersebut tergolong lemah (Umaru et al, 2018). Tingkat toksisitas yang lemah, dapat dikaitkan dengan kandungan senyawa fitokimia pada suatu sediaan.…”

Toksisitas Akut Ekstrak Etanol Daun Rumput Knop (Hyptis capitata Jacq.) dengan Metode Brine Shrimp Lethality Test (BSLT)

“…Nilai LC 50 suatu sediaan/bahan yang lebih kecil dari 1000µg/ml, selain menujukkan bahwa sediaan tersebut toksik, juga menunjukkan potensinya sebagai antitumor (Umaru et al, 2018). Metode uji toksisitas akut dengan Brine Shrimp Lethality Test merupakan tahapan awal untuk penapisan senyawa bioaktif yang memiliki potensi sebagai antitumor (Marraskuranto et al, 2008).…”