Cytotoxicity and selectiveness of Brazilian Piper species towards oral carcinoma cells

Macedo, Arthur L.; Silva, Diego P.D. da; Moreira, Davyson L.; Queiroz, Lucas Nicolau de; Vasconcelos, Thatyana R. A.; Araujo, Geisoellen Felicio; Kaplan, Maria Auxiliadora Coelho; Pereira, Suiane S.C.; Almeida, Elan Cardozo Paes de; Valverde, Alessandra L.; Robbs, Bruno Kaufmann

doi:10.1016/j.biopha.2018.11.129

Cited by 23 publications

(15 citation statements)

References 42 publications

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“…Therefore, both postulated hypotheses were accepted. For cytotoxicity assessment, MTT assay was employed, as the technique was convenient, rapid, and reproducible [17]. Similarly, immunofluorescence was used to determine α-SMA positive staining in fibroblastic cells, as it showed high sensitivity and specificity over other techniques [18].…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic Influence of Khat (Catha edulis (Vahl) Forssk. ex Endl) on Oral Fibroblasts, Squamous Carcinoma Cells, and Expression of α Smooth Muscle Actin

et al. 2021

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The aim was to determine the cytotoxicity of Khat (Catha edulis (Vahl) Forssk. ex Endl) on normal oral fibroblasts (NOFs) and SCC4 (squamous carcinoma cells) along with expression of α-smooth muscle actin (α-SMA) in fibroblasts. Khat filtrate was prepared to obtain a concentrated viscous solution. NOFs and SCC4 cells were cultured in biological cabinets and were grown in Dulbeccos’ modified Eagles medium. Frozen cells were thawed at 37 °C and cell seeding was per-formed. NOFs and SCC4 cells were seeded on 96 well plates and allowed to attach. The medium was removed and a fresh medium containing different concentrations of Khat was added. The group without Khat served as a negative control and 4% paraformaldehyde as the positive control. Cell viability was assessed using the MTT assay and effect of Khat on fibroblast and SCC4 pheno-types was evaluated by immunostaining. Analysis of variance was used to assess data (p < 0.05). NOF 316 showed cell death in response to 4% paraformaldehyde, 12.5, 6.25, and 3.12 mg/mL of Khat. The highest concentration of Khat (25 mg/mL) failed to cause cytotoxicity of NOF 316. NOF 319 and NOF 26 displayed cell death at all concentrations of Khat, however, cytotoxicity was not dose dependent. NOF 18 and SCC4 cells showed dose-dependent cell death. NOF 316 showed α-SMA expression after 1 mg/mL of Khat exposure. Not all fibroblasts were α-SMA-positive, sug-gesting specific activation of a subset of fibroblasts. Khat is cytotoxic to NOF and SCC4 cells. Fur-thermore, it can also cause activation and phenotypic changes in oral fibroblasts, indicating a po-tential role in progression of oral squamous cell carcinoma.

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Section: Discussionmentioning

confidence: 99%

Cytotoxic Influence of Khat (Catha edulis (Vahl) Forssk. ex Endl) on Oral Fibroblasts, Squamous Carcinoma Cells, and Expression of α Smooth Muscle Actin

et al. 2021

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“…It is important to highlight that to continue exploring plant extracts that could be used as therapeutics in humans in the future, it is important to perform toxicity tests. Although these tests were not carried out in this study, previous research has already demonstrated reduced toxicity of P. arboreum and J. gossypiifolia extracts (Nagaharika et al ., 2013; Macedo et al ., 2019). However, more tests are needed to be carried out to confirm the absence of toxicity of the extracts used in this study in non-target organisms and cells.…”

Section: Discussionmentioning

confidence: 99%

Antiparasitic effects of ethanolic extracts of Piper arboreum and Jatropha gossypiifolia leaves on cercariae and adult worms of Schistosoma mansoni

et al. 2020

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New treatment strategies for schistosomiasis should be evaluated, since resistant strains to the only available drug, Praziquantel, have already been described. Thus, we demonstrated antiparasitic effects of ethanolic extracts of Jatropha gossypiifolia and Piper arboreum on cercariae and adult worms of Schistosoma mansoni. The bioassays were performed at 0–10 000 μg mL−1 concentration for 0–72 h. Adult worms were stained with carmine to assess external and internal damage. The chemical screening was performed using high-performance liquid chromatography. P. arboreum displayed the best cercaricidal effect, with a 100% reduction in viability in just 60 min. The extract of J. gossypiifolia was more effective against adult worms, with 100% viability reduction of male and female worms after 12 and 24 h, respectively. P. arboreum and J. gossypiifolia were equally effective in inhibiting the oviposition of S. mansoni (93% reduction) and causing damage to internal and external structures in adult worms. Flavonoids were identified in both the extracts and phenolic compounds and amides only in P. arboreum. Thus, for the first time, it was proven that ethanolic extracts of P. arboreum and J. gossypiifolia leaves are biologically active against cercariae and adult worms of S. mansoni in vitro.

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“…The three fractions PM (-L-D), PCa (-L-D) and PC (-L-D) (crude methanolic extract of leaves) with OSCC cell treatment showed toxicity, with IC 50 values of 47.2, 94.2, and 47.5 µg/mL, respectively. An in vivo toxicology analysis of the PC-L-D fraction showed no significant alterations and less than 5% haemolysis [ 64 ]. Original photographs of a few important discussed plants are shown in Figure 3 .…”

Section: Plants With Beneficial Effects Against Oral Cancermentioning

confidence: 99%

Plant-Based Antioxidant Extracts and Compounds in the Management of Oral Cancer

et al. 2021

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Oral cancer continues to be a leading cause of death worldwide, and its prevalence is particularly high in developing countries, where people chew tobacco and betel nut on a regular basis. Radiation-, chemo-, targeted-, immuno-, and hormone-based therapies along with surgery are commonly used as part of a treatment plan. However, these treatments frequently result in various unwanted short- to long-term side effects. As a result, there is an urgent need to develop treatment options for oral cancer that have little or no adverse effects. Numerous bioactive compounds derived from various plants have recently attracted attention as therapeutic options for cancer treatment. Antioxidants found in medicinal plants, such as vitamins E, C, and A, reduce damage to the mucosa by neutralizing free radicals found in various oral mucosal lesions. Phytochemicals found in medicinal plants have the potential to modulate cellular signalling pathways that alter the cellular defence mechanisms to protect normal cells from reactive oxygen species (ROS) and induce apoptosis in cancer cells. This review aims to provide a comprehensive overview of various medicinal plants and phytoconstituents that have shown the potential to be used as oral cancer therapeutics.

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Cytotoxicity and selectiveness of Brazilian Piper species towards oral carcinoma cells

Cited by 23 publications

References 42 publications

Cytotoxic Influence of Khat (Catha edulis (Vahl) Forssk. ex Endl) on Oral Fibroblasts, Squamous Carcinoma Cells, and Expression of α Smooth Muscle Actin

Cytotoxic Influence of Khat (Catha edulis (Vahl) Forssk. ex Endl) on Oral Fibroblasts, Squamous Carcinoma Cells, and Expression of α Smooth Muscle Actin

Antiparasitic effects of ethanolic extracts of Piper arboreum and Jatropha gossypiifolia leaves on cercariae and adult worms of Schistosoma mansoni

Plant-Based Antioxidant Extracts and Compounds in the Management of Oral Cancer

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