Cytotoxicity and oxidative DNA damage by nanoparticles in human intestinal Caco-2 cells

Gerloff, Kirsten; Albrecht, Catrin; Boots, Agnes W.; Förster, Irmgard; Schins, Roel P. F.

doi:10.3109/17435390903276933

Cited by 241 publications

(175 citation statements)

References 36 publications

Supporting

Mentioning

158

Contrasting

Unclassified

Order By: Relevance

“…It is well known that ROS can interact with biomolecules including DNA and causes DNA single or double strand breaks and it is also well known that MDA causes free radicals. Some studies have reported similar results (Jeng and Swanson 2006;Musarrat et al 2009;Lin et al 2009;Gerloff et al 2009;Zhu et al 2009;Kim et al 2010). Our findings also clearly demonstrate that ZnO is clastogenic in human lymphocytes in vitro.…”

Section: Discussionsupporting

confidence: 78%

In vitro genotoxic effects of ZnO nanomaterials in human peripheral lymphocytes

et al. 2013

View full text Add to dashboard Cite

In this study, possible genotoxic effects of zinc oxide (ZnO) nanoparticles were investigated in cultured human peripheral lymphocytes by using chromosome aberrations and micronucleus assays (MN). For this purpose, the cells were treated with ZnO (1, 2, 5, 10, 15 and 20 lg/mL) for 24 and 48 h. In this research, four types of chromosome aberrations were observed as chromatid and chromosome breaks, fragment and dicentric chromosomes. ZnO induced significant increase of the ratio of chromosomal aberrations as well as percentage of abnormal cells at concentrations of 1, 5, 10 and 20 lg/mL in 24 h treatments. In 48 h treatments, while ZnO nanomaterials induced significant increase of the percentage of abnormal cells only at a concentration of 10 lg/mL, and of chromosome aberration per cell in comparison to the control at concentrations of 5 and 10 lg/mL. On the other hand, this material significantly increased the micronuclei frequency (MN) at concentrations of 10 and 15 lg/mL in comparison to the control. Cytokinesis-block proliferation index was not affected by ZnO treatments. It also decreased the mitotic index in all concentrations at 24 h but not at 48 h. The present results indicate that ZnO nanoparticles are clastogenic, mutagenic and cytotoxic to human lymphocytes in vitro at specific concentrations and time periods.

show abstract

Section: Discussionsupporting

confidence: 78%

In vitro genotoxic effects of ZnO nanomaterials in human peripheral lymphocytes

et al. 2013

View full text Add to dashboard Cite

show abstract

“…[67][68][69][70][71] The ability of TiO 2 nanoparticles to exhibit these toxic effects has been attributed to numerous mechanisms, including generation of ROS species that damage key cellular constituents, interference with efflux pumps and nutrient transporters, induction of inflammation, and alteration of the gut microbiota. 12,72,73 Anatase TiO 2 nanoparticles were reported to be more toxic to cells than rutile nanoparticles due to their higher photo-catalytic activity.…”

Section: Inorganic Nanoparticlesmentioning

confidence: 99%

Is nano safe in foods? Establishing the factors impacting the gastrointestinal fate and toxicity of organic and inorganic food-grade nanoparticles

2017

View full text Add to dashboard Cite

Nanotechnology offers the food industry a number of new approaches for improving the quality, shelf life, safety, and healthiness of foods. Nevertheless, there is concern from consumers, regulatory agencies, and the food industry about potential adverse effects (toxicity) associated with the application of nanotechnology in foods. In particular, there is concern about the direct incorporation of engineered nanoparticles into foods, such as those used as delivery systems for colors, flavors, preservatives, nutrients, and nutraceuticals, or those used to modify the optical, rheological, or flow properties of foods or food packaging. This review article summarizes the application of both inorganic (silver, iron oxide, titanium dioxide, silicon dioxide, and zinc oxide) and organic (lipid, protein, and carbohydrate) nanoparticles in foods, highlights the most important nanoparticle characteristics that influence their behavior, discusses the importance of food matrix and gastrointestinal tract effects on nanoparticle properties, emphasizes potential toxicity mechanisms of different food-grade nanoparticles, and stresses important areas where research is still needed. The authors note that nanoparticles are already present in many natural and processed foods, and that new kinds of nanoparticles may be utilized as functional ingredients by the food industry in the future. Many of these nanoparticles are unlikely to have adverse affects on human health, but there is evidence that some of them could have harmful effects and that future studies are required.

show abstract

“…In previous study, exposure of human bronchoalveolar carcinomaderived cells to SiO 2 nanoparticles revealed doseand time-dependent cytotoxicity (Lin et al 2006). Mechanistic studies suggest that the cytotoxicity is due to elevated oxidative stress, oxidative DNA damage and lipid peroxidation (Gurr et al 2005, Gerloff et al 2009, Asadpour et al 2016. In this study, we investigated silicon nanoparticles (as silicon carbide, silicon dioxide, silicon nitride)-mediated changes in cellular pathway-specific gene expression associated with DNA damage or repair (ATM, Rad23 and Rad50), apoptosis (Anxa5 and Fasl) and oxidative stress (Gpx2, Gs, Mt2, Cyp4a10) in human alveolar epithelial (HPAEpiC) and pharynx (HPPC) cell lines.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Cytotoxicity and Molecular Effects Related to Silicon Nanoparticles Exposures

Aydın¹,

Türkez²,

Hacımüftüoğlu³

2017

AKU-J. Sci. Eng.

View full text Add to dashboard Cite

Silicon nanoparticles are widely used for various applications including environmental, biological, chemical and physical. And, to translate these nanomaterials to the clinic and industrial domains, their safety needs to be verified, particularly in terms of genotoxicity and cytotoxicity. Therefore, in this study, we aimed to investigate of cytotoxicity and changes in gene expression profiles influenced by commonly silicon (as silicon carbide, silicon dioxide, silicon nitride) nanoparticles in human alveolar epithelial (HPAEpiC) and pharynx (HPPC) cell lines in vitro since inhalation is an important pathway for exposure to these nanoparticles. HPAEpiC and HPPC cells were treated with silicon (0-100 µg/mL), nanoparticles for 72 h, and then cytotoxicity was detected by, [3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide] (MTT) and lactate dehydrogenase (LDH) release assays, while genotoxicity was also analyzed by cDNA array -RT-PCR assay. According to the results of MTT and LDH assays, all tested nanoparticles induced cytotoxicity on both HPAEpiC and HPPC cells in dose-dependent manner. Determining and analyzing the gene expression profiles of HPAEpiC and HPPC cells, silicon nanoparticles showed changes in genes related to apoptosis, DNA damage or repair and oxidative stress. This study of gene expression profiles affected by nanotoxicity provides critical information for the clinical and environmental applications of silicon nanoparticles. Silikon Nanopartikül Maruziyetine Bağlı Olarak Oluşan İn Vitro Sitotoksik ve Moleküler Etkiler Anahtar kelimelerAlveolar epitel hücreleri; Genotoksisite; In vitro gen ekspresyonu; Nanotoksisite; Silikon nanopartikülleri ÖzetSilikon nanopartikülleri çevresel, biyolojik, kimyasal ve fiziksel amaçlarla çeşitli alanlarda yaygın olarak kullanılmaktadır. Bu nanopartiküllerin klinik ve endüstriyel alanlarda kullanılabilmesi için güvenilirlikleri özellikle genotoksisite ve sitotoksisite açısından doğrulanmalıdır. Bu yüzden mevcut çalışmada, yaygın olarak kullanılan silikon nanopartiküllerinin ( silikon karbid, silikon dioksit, silikon nitrit) insan alveolar epitel (HPAEpiC) ve farinks (HPPC) hücrelerindeki sitotoksisitesi ve gen ekspresyon profillerindeki değişimlerin araştırılması amaçlanmıştır. HPAEpiC ve HPPC hücreleri 72 saat boyunca silikon nanopartikülleriyle (0-100 µg/mL) muamele edildi. Nanopartiküllerin sitotoksisite değerlendirmeleri için 3-(4,5 dimetylthiazol -2-yl) -2,5 diphenltetrazolium bromide (MTT) ve laktat dehidrogenaz salınım (LDH) yöntemleri kullanılırken; genotoksisite analizi için cDNA array -RT-PCR yöntemi kullanıldı. MTT ve LDH yöntemi sonuçlarına göre, uygulanan bütün test nanopartikülleri hem HPAEpiC hem de HPPC hücre hatlarında doza bağlı olarak sitotoksisiteyi indüklemiştir. HPAEpiC ve HPPC hücrelerinin ilgili genler açısından (apoptozis, DNA fasarı ve tamiri, oksidatif stres) gen ekspresyon profilleri incelendiğinde silikon naopartiküllerinin ekspresyonu değiştirdiği gözlenmiştir. Bu çalışmadan elde edilen nanotoksisiteye bağlı olarak ol...

show abstract

Cytotoxicity and oxidative DNA damage by nanoparticles in human intestinal Caco-2 cells

Cited by 241 publications

References 36 publications

In vitro genotoxic effects of ZnO nanomaterials in human peripheral lymphocytes

In vitro genotoxic effects of ZnO nanomaterials in human peripheral lymphocytes

Is nano safe in foods? Establishing the factors impacting the gastrointestinal fate and toxicity of organic and inorganic food-grade nanoparticles

In Vitro Cytotoxicity and Molecular Effects Related to Silicon Nanoparticles Exposures

Contact Info

Product

Resources

About