Cytotoxicity and Genotoxicity of Biosynthesized Gold and Silver Nanoparticles on Human Cancer Cell Lines

Parveen, Asra; Rao, Srinath

doi:10.1007/s10876-014-0744-y

Cited by 57 publications

(25 citation statements)

References 48 publications

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“…Therefore, it can be hypothesized that growth of cells were arrested at G1 phase and lead to apoptosis. This study was in concordance with study done by Prabhu et al (2013), which reported AgNps caused colon cancer cells (HCT-15) growth arrested at G0/G1 phase after treated with 20 mg/mL at 48 h incubation time [23]. Study done by Palaniselvam et al (2015) reported AgNps inhibit growth of colon cancer cells (HCT-116) and increased in proportion of Sub-G1 phase in all treated samples compared to control group [7].…”

Section: Discussionsupporting

confidence: 93%

“…Study by Sankar et al, (2013) reported anticancer effects of silver nanoparticle against human lung cancer cell lines (A549) with 50% of cell death at 100 mg/mL [22]. Various concentrations from 5 mg/mL-500 mg/mL of silver nanoparticle can be seen to exhibit cytotoxicity effects against various cultured cancer cells such as MCF-7, Hela, HepG2 and HT29 cell lines [23,24]. Therefore, AgNps in this study exhibited higher potential compared to other studies mentioned above with lower IC 50 and 90% of cell death were found when treated at 3 mg/mL.…”

Section: Discussionmentioning

confidence: 99%

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Silver nanoparticles Clinacanthus Nutans leaves extract induced apoptosis towards oral squamous cell carcinoma cell lines

Yakop

Ghafar

Yong

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Silver nanoparticles Clinacanthus Nutans leaves extract induced apoptosis towards oral squamous cell carcinoma cell lines

Yakop

Ghafar

Yong

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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“…These findings suggest that the higher concentration of silver nanoparticles can induce toxicity and can affect the whole genome resulting into the DNA damage [ 63 , 64 ]. Previous reports [ 30 , 26 , 31 ] on mammalian and plant cells have proved that AgNPs accumulate in cytoplasm and may lead to toxic effect on mitochondria and nucleus, causing uncoupling of respiration and increased oxidative stress which leads to DNA breakage, base modification and cross linking of DNA damage, which can be repaired with or without cell cycle arrest [ 65 , 66 ]. The present study demonstrated that the frequency of chromosomal aberrations is inversely proportional to the mitotic index (MI) with increased concentrations of AgNPs in a dose dependent manner, which results in DNA breakage, inhibition of DNA synthesis and altered DNA replication [ 67 ].…”

Section: Resultsmentioning

confidence: 99%

Characterization and cytotoxic effect of biogenic silver nanoparticles on mitotic chromosomes of Drimia polyantha (Blatt. & McCann) Stearn

Daphedar

Taranath

2018

Toxicology Reports

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“…Many researchers have demonstrated that gold NPs exhibit less cytotoxic and genotoxic effects compared to other nanomaterials like Zn and Ag NPs 36,37 . However, research in Drosophila melanogaster reported the gold NPs induces decreased infertility and numbers of progeny, increased in aberrant phenotype 38 .…”

Section: Discussionmentioning

confidence: 99%

The nervous system is the major target for Gold nanoparticles: Evidence from RNA sequencing data of C. elegans

Sun

Yang

et al. 2019

Preprint

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12The increasing use of gold nanoparticles (NPs) raises concerns about the potential effect of gold NPs 13 exposure on human health. Therefore, gold NPs exposure is hard to evaluate at the organ level with 14 current measurement technology. The bio-distribution assay showed that intestine was the organ with 15 most gold NPs accumulated. However, our data indicated that 62.8% of the significant altered genes 16 were function in the nervous system using tissue enrichment analysis. Notably, gene ontology analysis 17 has demonstrated that NP exposure interfered with the development of animals. Furthermore, the 18 transcription factors DAF-16 and HSF-1 were regulating the oxidative stress genes induced by gold NPs. 19Therefore, we proposed the localization of the oxidative stress genes in the neuron cells and how their 20 expression affect neuron communication. Our results demonstrate that the gold NPs-induced oxidative 21 stress affects the nervous system via physical damage to the neurons and disruption of cell-to-cell 22 communication. Future toxicology research on gold NPs should focus on neurons. 23 24 25 26 55 Studies in mice show that 13 nm PEG-coated gold NPs were mainly deposited in the liver, kuffer cells, 56 and the spleen 21 . While other studies in rats proved 18 nm gold NPs were accumulated in the liver and 57 spleen, but 1.4 nm mainly in the kidney 22 . The intestine of C. elegans, have the combined function of 58 liver, spleen and kidney, accumulates most of the gold NPs 23 . Although these organs accumulate gold 59 NPs, further investigations should be focused on how each organ responds to the gold NPs, and how the 60 expression of genes in each tissues restore homeostasis. 62C. elegans is a transparent animal with multiple important signaling pathways conserved in mammals, 63 like autophagy, insulin, and apoptosis signaling pathways 24 . We analyzed the RNA sequencing data set 64 (GSE32521) from gold NPs exposed worm. However, different with the biodistribution data, the results 65 showed that the nerve system was the most affected when exposing organisms to gold NPs. Further 66 studies show that the oxidative stress transcription factor DAF-16 and HSF-1 are the main transcription 67 factors that regulate the gold NP-induced gene expressions. The ion-channel protein analysis indicated 68 that the potassium and solute carrier (SLC) channels are the most altered channels by gold NPs, which 69 could be the direct way of how gold NPs affect neuron activity. 70 5 71 Materials and Methods 72RNA sequencing data Analysis 73The RNA sequencing data, reference series GSE32521, was obtained from Gene Expression Omnibus, 74National Center for Biotechnology Information. Age-synchronized L3 worms were exposed to 4 nm gold 75NPs with a concentration of 5.9 mg/L in 50% K-Medium. Then they were cultured with 50% K-Medium 76 and left for another 12h. The RNA was extracted from each of the replicates. Significantly changed genes 77 with 1.5 fold and p < 0.05 and FDR < 0.1 were analyzed 23 . 79Gene Expression Analysis 80...

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Cytotoxicity and Genotoxicity of Biosynthesized Gold and Silver Nanoparticles on Human Cancer Cell Lines

Cited by 57 publications

References 48 publications

Silver nanoparticles Clinacanthus Nutans leaves extract induced apoptosis towards oral squamous cell carcinoma cell lines

Silver nanoparticles Clinacanthus Nutans leaves extract induced apoptosis towards oral squamous cell carcinoma cell lines

Characterization and cytotoxic effect of biogenic silver nanoparticles on mitotic chromosomes of Drimia polyantha (Blatt. & McCann) Stearn

The nervous system is the major target for Gold nanoparticles: Evidence from RNA sequencing data of C. elegans

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