Characterization and cytotoxic effect of biogenic silver nanoparticles on mitotic chromosomes of Drimia polyantha (Blatt. &amp; McCann) Stearn

Daphedar, Azharuddin; Taranath, T. C.

doi:10.1016/j.toxrep.2018.08.018

Cited by 36 publications

(25 citation statements)

References 63 publications

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“…The results of the Allium cepa assay were consistent with those obtained in a similar trial using the plant species Drimia polyantha , where decreases of the mitotic index and the presence of chromosomal alterations were found following exposure to the highest concentrations of biogenic silver nanoparticles synthesized using the plant Getonia floribunda 61 . Similar results were obtained using the Allium cepa assay to evaluate the effects of low concentrations of silver nanoparticles synthesized using the plant Swertia chirata 62 .…”

Section: Resultssupporting

confidence: 85%

Biosynthesis of silver nanoparticles employing Trichoderma harzianum with enzymatic stimulation for the control of Sclerotinia sclerotiorum

Guilger-Casagrande

Germano-Costa

Pasquoto-Stigliani

et al. 2019

Sci Rep

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Biogenic synthesis of silver nanoparticles employing fungi offers advantages, including the formation of a capping from fungal biomolecules, which provides stability and can contribute to biological activity. In this work, silver nanoparticles were synthesized using Trichoderma harzianum cultivated with (AgNP-TS) and without enzymatic stimulation (AgNP-T) by the cell wall of Sclerotinia sclerotiorum. The nanoparticles were evaluated for the control of S. sclerotiorum. The specific activity of the T. harzianum hydrolytic enzymes were determined in the filtrates and nanoparticles. Cytotoxicity and genotoxicity were also evaluated. Both the nanoparticles exhibited inhibitory activity towards S. sclerotiorum, with no new sclerotia development, however AgNP-TS was more effective against mycelial growth. Both the filtrates and the nanoparticles showed specific enzymatic activity. Low levels of cytotoxicity and genotoxicity were observed. This study opens perspectives for further exploration of fungal biogenic nanoparticles, indicating their use for the control of S. sclerotiorum and other agricultural pests.

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Section: Resultssupporting

confidence: 85%

Biosynthesis of silver nanoparticles employing Trichoderma harzianum with enzymatic stimulation for the control of Sclerotinia sclerotiorum

Guilger-Casagrande

Germano-Costa

Pasquoto-Stigliani

et al. 2019

Sci Rep

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“…There are also some reports on the genotoxic effects of AgNPs in Tradescantia paludosa, Vicia faba, Hordeum vulgare, Crepis capillaries, Pisum sativum, Allium sativum, Drimia indica, etc. [27,28]. Researchers have revealed that the A. cepa system is considered to be the best-established system, which is correlated considerably with the studies performed in eukaryotic and prokaryotic systems for evaluation of genotoxicity in vitro and in vivo [29].…”

Section: Introductionmentioning

confidence: 89%

Biosynthesis, characterization, and in vitro assessment on cytotoxicity of actinomycete-synthesized silver nanoparticles on Allium cepa root tip cells

Nayaka

Chakraborty

Bhat

et al. 2020

Beni-Suef Univ J Basic Appl Sci

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Background The industrial production of silver nanoparticles (AgNPs) and its commercial applications are being considerably increased in recent times, resulting in the release of AgNPs in the environment and enhanced probability of contaminations and their adverse effects on living systems. Based on this, the present study was conducted to evaluate the in vitro cytotoxicity of actinomycete-synthesized AgNPs on Allium cepa (A. cepa) root tip cells. A green synthesis method was employed for biosynthesis of AgNPs from Streptomyces sp. NS-33. However, morphological, physiological, biochemical, and molecular analysis were carried out to characterize the strain NS-33. Later, the synthesized AgNPs were characterized and antibacterial activity was also carried out against pathogenic bacteria. Finally, cytotoxic activity was evaluated on A. cepa root tip cells. Results Results showed the synthesis of spherical and polydispersed AgNPs with a characteristic UV-visible (UV-Vis.) spectral peak at 397 nm and average size was 32.40 nm. Energy dispersive spectroscopy (EDS) depicted the presence of silver, whereas Fourier transform infrared (FTIR) studies indicated the presence of various functional groups. The phylogenetic relatedness of Streptomyces sp. NS-33 was found with Streptomyces luteosporeus through gene sequencing. A good antibacterial potential of AgNPs was observed against two pathogenic bacteria. Concerning cytotoxicity, a gradually decreased mitotic index (MI) and increased chromosomal aberrations were observed along with the successive increase of AgNPs concentration. Conclusions Therefore, the release of AgNPs into the environment must be prevented, so that it cannot harm plants and other beneficial microorganisms.

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“…Contrarily, accumulating evidences showed AgNP-induced toxicological effects in aquatic ecosystem, causing lethal damage to aquatic plants, aquatic microbes and aquatic vertebrates [33], as well as bacteria and non-mammalian animal species [17,34]. In addition, several investigations using in vitro models, further demonstrated size-, dose- and cell-dependent nanosilver cytotoxicity and genotoxicity, including chromosomal abnormalities and inhibition of mitotic processes [17,[35], [36], [37], [38], [39]].…”

Section: Introductionmentioning

confidence: 99%

Pulmonary and hepatic effects after low dose exposure to nanosilver: Early and long-lasting histological and ultrastructural alterations in rat

Roda

Biggiogera

et al. 2019

Toxicology Reports

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Characterization and cytotoxic effect of biogenic silver nanoparticles on mitotic chromosomes of Drimia polyantha (Blatt. & McCann) Stearn

Cited by 36 publications

References 63 publications

Biosynthesis of silver nanoparticles employing Trichoderma harzianum with enzymatic stimulation for the control of Sclerotinia sclerotiorum

Biosynthesis of silver nanoparticles employing Trichoderma harzianum with enzymatic stimulation for the control of Sclerotinia sclerotiorum

Biosynthesis, characterization, and in vitro assessment on cytotoxicity of actinomycete-synthesized silver nanoparticles on Allium cepa root tip cells

Pulmonary and hepatic effects after low dose exposure to nanosilver: Early and long-lasting histological and ultrastructural alterations in rat

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