Cytotoxicity and Genotoxicity Analysis of two Endodontic cements in Human Fibroblast Culture in Vitro

Abstract

The present in vitro study aims to evaluate cytotoxic and genotoxic potential of MTA Fillapex endodontic sealer and to compare it with AH Plus sealer. It was used human fibroblast cell lines FG11 and FG15 for this study. Cytotoxicity and genotoxicity was analysed in human gingival fibroblast submitted to growth condition with MTT test conditioned cells, respectively. Cells cultivated in DMEM means was used as command. Celular viability was mensured in 24, 48 and 72h. Results was analysed by the software Biostat 4.0 Shapiro-Wilk normality test was made but sample presented non-normal behavior. Descriptive analysis was made and its results was submitted to Kruskal-Wallis test (Dunn). All sealers and control groups presented MTT values lower in 24h period than 48 and 72h (p<0.05). The biggest cell viability was observed in AH Plus sealer and in control group related to MTA Fillapex in all experimental periods (p≤0.0002). In terms of genotoxicity, the biggest value was mensuared in AH Plus sealer in the 24h period with significantly difference compared to MTA Fillapex and the control group (p=0.0004). It may be concluded that MTA Fillapex sealer showed higher cell cytotoxicity than two control groups and AH Plus sealer presented higher genotoxicity than other groups.

In vitro toxicogenomic activity of an MTA/salicylate-based endodontic sealer

“…The MTA Fillapex® endodontic sealer tested in the present study had no genotoxic effect on HGFs. Using a modified micronucleus test without cytokinesis-blocking, no mutagenicity of the cement was also detected in human fibroblast cell lines FG11 and FG15 [31] . Previously, neither regular nor white MTAs were found to be genotoxic in Chinese hamster ovary (CHO) cells or in vitro human peripheral lymphocytes obtained from 10 healthy volunteers.…”