“…Regarding the number of cells evaluated in the micronucleus assay, a total of five studies evaluated 1,000 cells per slide. For the alkaline comet assay, a total of three studies evaluated 100 randomly selected comets per slide, totaling 300 comets ( Teixeira et al, 2021 , Baraba et al, 2011 , Dhopavkar et al, 2021 ), whereas 50 comets per treatment were evaluated in four studies ( Leme and Salvadori, 2022 , Huang et al, 2002 , Huang et al, 2004 , Dhopavkar et al, 2021 ). The study conducted by Nair et al (2018) evaluated from 50 to 100 cells per sample, whereas the study by Camargo et al (2014) evaluated only 25 cells and three studies failed to inform the amount of analyzed cells.…”