Cytotoxicity and fibroblast properties during in vitro test of biphasic calcium phosphate/poly‐dl‐lactide‐co‐glycolide biocomposites and different phosphate materials

Abstract

Reconstruction of bone defects is one of the major therapeutic goals in various clinical fields. Bone replacement materials must satisfy a number of criteria. Biological criteria are biocompatibility, controlled biodegradability, and osteoconductive or even osteogenic potential. The material should have a three-dimensional structure with an interconnected pore system so as to permit cell growth and transport of substances. The surface must permit cell adhesion and proliferation. Composite biomaterials have enormous potential for natural bone tissue reparation, filling and augmentation. Calcium hydroxyapatite/polymer composite biomaterials belong to this group of composites and, because of their osteoconductive and biocompatible properties, can be successfully implemented within bone tissue reparations. In this study, possible differences between BCP/DLPLG, pure BCP, and Bio-Oss materials were examined in vitro. During overnight incubations, fibroblast and fibroblast-like cells (L929, MRC5) were able to adhere, spread, and remain viable on BCP, BCP/PLGA, and Bio-Oss discs, as was evidenced by using light- and LVSEM-microscopy. Inhibiting influence over the cell growth is more pronounced in the cases of BCP usage on both cell lines--41.29% for L929 and 43.08% for MRC-5 cells. MRC-5 cells are, within the given experimental conditions, less sensitive on inhibiting effects for the materials BCP/PLGA and Bio-Oss (10.13% and 10.76%, respectively) than for the L929 cell lines (23.02% and 15.44%, respectively).

Comparative study of a new composite biomaterial fluor-hydroxyapatite on fibroblast cell line NIH-3T3 by direct test

“…Most cell lines of mesenchymal origin, for instance fibroblasts, without specialized persistent metabolic functions demonstrate reactions to toxic agents based on basal cell functions (Barile 1994). The L929 clone of mouse fibroblasts is commonly used to assess the cytotoxicity of an experimental material for medical applications (ISO 10993-5;Rice et al 1978;Vallittu & Ekstrand 1999;Liskorish et al 2004;Nablo & Schoenfish 2005;Ignjatovič et al 2006). Mouse L929 and Balb/c fibroblasts are appropriate cell models for the study of cellular basal cytotoxicity because of their easiness to maintain and good correlation with animal tests (INVITTOX protocols no.38,46).…”

Nanosized hydroxyapatite and other calcium phosphates: Chemistry of formation and application as drug and gene delivery agents

“…Polymer matrices have thus been reinforced with HAP nanocrystals to improve the mechanical properties of these polymers. 306,307 Poly-L-lactide, 308 poly(lactic-co-glycolic acid) (PLGA) 309,310,311 and collagen 312 all present prospective choices as polymeric phases that promote cellular adhesion, proliferation and growth. In fact, inorganic nanoparticle fillers in general have been shown to add tensile strength, stiffness, abrasion resistance, crack resistance, and stability to polymer networks.…”

Biocomposites and hybrid biomaterials based on calcium orthophosphates

“…491 More relevantly, both PLGA/BCP 492,493 and PLLA/ BCP 494 biocomposites were fabricated, and their cytotoxicity and fibroblast properties were found to be acceptable for natural bone tissue reparation, filling and augmentation. 495,496 PCL/BCP 497 and gelatin/BCP 498,499 biocomposites are known as well. A choice of DCPD-based biocomposites of DCPD, albumin and duplex DNA was prepared by a water/oil/water interfacial reaction method.…”