Cytotoxicity and cytokine expression induced by silorane and methacrylate-based composite resins

Abstract

The successful use of composite resins in Dentistry depends on physicochemical properties, but also on the biological compatibility of resins, because of the close association between pulp and dentin. Objective The aim of this study was to evaluate cytotoxicity and cytokine production induced by light-cured or non-light-cured methacrylate-based and silorane composite resins in RAW 264.7 macrophages.Material and Methods Cells were stimulated with the extracts from light-cured or non-light-cured composite resins. After incubation for 24 h, cytotoxicity was assessed with the lactate dehydrogenase (LDH) and methyl thiazolyl tetrazolium (MTT) assays, and total protein was quantified using the Lowry method. TNF-α detection was examined with an enzyme-linked immunosorbent assay (ELISA) conducted with cell supernatants after cell stimulation for 6, 12, and 24 h. Data were analyzed using one-way analysis of variance (ANOVA) and Tukey’s post hoc test (α=0.05).Results KaloreTM and FiltekTM Silorane were cytotoxic with or without light curing (p<0.05) after 24 h of incubation. KaloreTM stimulated the early production of TNF-α in comparison with control (p<0.05), whereas FiltekTM Silorane did not affect TNF-α levels after 6 and 12 h (p>0.05). However, after 24 h FiltekTM Silorane inhibited the production of TNF-α (p<0.05).Conclusions KaloreTM and FiltekTM Silorane were cytotoxic regardless of light curing. The extract obtained from KaloreTM after 15 days of incubation stimulated the production of TNF-α, unlike that obtained from FiltekTM Silorane.

Cytotoxic and biological effects of bulk fill composites on rat cortical neuron cells

“…It was reported that the contact period was another effective factor on the cytotoxicity of the materials. [ 19 , 65 ]. But, in the present study, cortical neuron cells are used instead of rat pulp cells [ 50 ].…”

“…Recently, in vitro and in vivo studies showed that monomers have been interrelated with genotoxicity [ 14 – 19 ], estrogenicity [ 14 , 17 , 19 , 20 ], immune system [ 19 , 21 , 22 ], hypersensitivity, cytotoxicity [ 14 , 15 , 23 , 24 ] and the production of reactive oxygen species [ 23 , 25 ]. Based on these literature data, it is necessary to know biological and chemical effects of dental materials on cells.…”

The biocompatibility of resin composite materials on different stem cells.

“…In a study evaluating the cytotoxocity and cytokine expression of silorane and methacrylate-based composite resins; it was proven that cytotoxicity was not observed in siloran-based composites within 6 h and 12 h. Also, the toxic symptoms were seen after 24 h [51]. In this study, there were a cell degeneration of approximately 13% in GFBCs and a cell degeneration of approximately 25% in CNCs after 72 h.…”

Investigation of the Cytotoxic Effect of Composite Materials on Gingival Fibroblast Cells

“…39 Longo ve ark., metakrilat bazlı nanohibrid yapıda (doldurucu oranı: %82) ve siloran bazlı mikrohibrid yapıda iki kompozitin (doldurucu oranı %76) hücre canlılığı üzerine etkisini değerlendirdikleri çalışmalarında ise monomer yüzdesi daha düşük olmasına rağmen nanohibrid yapıdaki metakrilat bazlı kompozitte daha düşük hücre canlılığı oranlarına rastlandığını bildirmişlerdir. 40 Gonçalves ve ark., farklı yapılardaki konvansiyonel ve bulk-fill kom- pozitlerin insan fibroblastları üzerindeki sitotoksisitesini inceledikleri bir çalışmada; en yüksek hücre canlılık oranı, en yüksek oranda doldurucu, en düşük oranda monomer içeren bulk-fill kompozitten elde edilmiş olup, monomer oranının doğrudan sitotoksisite üzerinde etkili olduğunu ortaya koymuşlardır. 41 Bununla birlikte, benzer monomer oranlarındaki konvansiyonel kompozitler ve bulkfill kompozitlerin eşit kalınlıkta polimerizasyonu sonucunda (4 mm) bulk-fill kompozitlerde daha yüksek hücre canlılığı elde edildiği saptanarak, konvansiyonel kompozitlerin 2 mm'den fazla kalınlıkta uygulanmaması gerektiğinin biyouyumluluk açısından da oldukça önem arz ettiği bilgisi doğrulanmıştır.…”