Cytotoxicity and cytochrome P450 inhibitory activities of <i>Clinacanthus nutans</i>

Quah, Suk Yen; Chin, Jin Han; Akowuah, Gabriel Akyirem; Khalivulla, Shaik Ibrahim; Yeong, Siew Wei; Sabu, M C

doi:10.1515/dmpt-2016-0034

Cited by 14 publications

(9 citation statements)

References 22 publications

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“…However, the same extract was much less toxic to normal 3T3 cells. Further analyses showed that the antiproliferative effects were possibly attributed to the inhibition in activities of CYP3A4 and CYP2E1, two liver enzymes for detoxifying drugs [ 19 ].…”

Section: Effects and Action Mechanism Studies Of C Nutans Extracts On Cancersmentioning

confidence: 99%

“…However, the methanolic extract was much less toxic to normal 3T3 cells. The antiproliferative effect was possibly through inhibition in the enzymatic activities of two detoxifying enzymes CYP3A4 and CYP2E1 [ 19 ]. Another study reported the preparation of C. nutans extracts with five different solvents (hexane, chloroform, ethyl acetate, methanol, and water) and examined the anticancer activities on HepG2 cells.…”

Section: Effects and Action Mechanism Studies Of C Nutans Extracts On Cancersmentioning

confidence: 99%

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Recent Advancement in Anticancer Activity of Clinacanthus nutans (Burm. f.) Lindau

Lin

Chen

Lung

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Clinacanthus nutans is a traditional medicinal herb that is applied for the therapy of snake bites, skin infection, herpes infection, burns, scalds, dysentery, and diabetes. Clinacanthus nutans is also used to treat several cancers, including breast, cervical, colon, gastric, head and neck, liver, lung, pancreatic, and skin cancers, as well as lymphoma and leukemia; however, the underlying mechanisms of its anticancer activity remained undetermined. We searched PubMed and Google with key words “Clinacanthus nutans and cancer” and collected recent papers of Clinacanthus nutans with anticancer activity. We focused on the preparation, effects, and action mechanisms of Clinacanthus nutans extracts on various types of cancers. We hope that this mini review can help update our knowledge about active components, effects, and molecular mechanisms of extracts from this promising herb Clinacanthus nutans for ongoing studies and speed up its clinical application in the future.

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Section: Effects and Action Mechanism Studies Of C Nutans Extracts On Cancersmentioning

confidence: 99%

Section: Effects and Action Mechanism Studies Of C Nutans Extracts On Cancersmentioning

confidence: 99%

Recent Advancement in Anticancer Activity of Clinacanthus nutans (Burm. f.) Lindau

Lin

Chen

Lung

et al. 2021

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

show abstract

“…Dried C. nutans leaves powder was bought from a nursery located in Kota Bharu, Kelantan andwas extracted according to the method described previously with slight modifications (Quah et al, 2017). For ethanolic extract, 50 mg of a powder sample in 500 mL of 70% ethanol was sonicated for 30 min.…”

Section: Extraction Of Clinacanthus Nutansmentioning

confidence: 99%

“…C. nutans has been widely used as an anticancer remedy without knowing its effects on chemotherapy. A recent study found that methanol extract of C. nutans at different concentrations possesses inhibitory activity on the metabolism of an analgesic drug (aminopyrine) by CYP3A4 (Quah et al, 2017). In this study, polar extracts of C. nutans were evaluated for their phytochemical profiles, antiproliferative activity, and also combination effect with cyclophosphamide on the normal liver cell line.…”

Section: Introductionmentioning

confidence: 99%

Antagonistic Drug-herb Interactions between Clinacanthus nutans and Cyclophosphamide on WRL 68 Cell Line

Mutalib¹,

Latip²

2020

psr

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Clinacanthus nutans (Acanthaceae), locally known as Sabah snake grass, is popularly taken as prevention as well as treatment for cancer in Malaysia, despite lack of concrete clinical evidence. However, it is crucial to evaluate potential antagonistic, additive, or synergistic interactions that may result from the co-treatment of this plant in chemotherapy. In this study, we demonstrate the drug-herb interaction using combination treatment of C. nutans extracts and cyclophosphamide on the WRL 68 cell line. Materials and Methods: Aqueous and 70% ethanol extract of C. nutans leaves were prepared using decoction and maceration methods. MTT assay was used to test single treatment as well as the combination of C. nutans extract and cyclophosphamide. Phytochemical profiling and flavonoid were identified using HPLC. Results: C. nutans ethanolic extract exhibits low antiproliferative activity. A combination of ethanolic extract of C. nutans and cyclophosphamide at various concentrations resulted in antagonism with combination index values of 1.413, 1.482, and 1.525. Flavonoids identified in the phytochemical profile of both extracts were schaftoside, isoorientin, orientin, and vitexin. High flavonoids level in C. nutans ethanolic extract could potentially interact with metabolic enzymes of the cell line, which might have affected metabolism and activated the cyclophosphamide. The in vitro data suggested that there was a potential for drug-herb interactions, which could negatively affect the chemotherapeutic outcomes. Further investigation should be done in vivo and clinical research model.

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“…Dried C. vespertilionis leaves powder was extracted according to method described previously 10 with slight modifications. For ethanolic extract, 50 g of powder sample in 500mL of 70% ethanol was sonicated for 30 minutes before solvent removal at 40 o C using rotary evaporator.…”

Section: Extraction Of C Vespertilionismentioning

confidence: 99%

Synergistic Interactions between Christia vespertilionis Leaves Extract and Chemotherapy Drug Cyclophosphamide on WRL-68 Cell Line

Latip

Mutalib

2019

Asian J Pharm Res Dev

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Christia vespertilionis locally known as ‘rerama’ has gained much attention as herbal preparation to treat various ailments and diseases although there is no conclusive evidence of its therapeutic values. Self-medication is commonly practiced by patients, underpinned by health believes that affect their adherence to medication regimens, and impacting on treatment outcomes. Therefore, in this study the phytochemical profile and potential interaction of this plant extracts with conventional chemotherapy drug cyclophosphamide were investigated. Water decoction and maceration method with 70% ethanol were used to extract the plant leaves. Phytochemical profiling of the extracts and compound identification was done using HPLC. Isoorientin was identified as the major phytochemical component of the extracts. MTT assay was used to screen cytotoxicity of the extract towards CRL2522, HaCaT, HepG2, MCF-7 and WRL68 cell lines. Combination index (CI) and isobologram were derived from combination treatment of extract and cyclophosphamide. Ethanolic extract has low cytotoxicity against all cell lines (IC50>1mg/mL). However, combination treatment of ethanolic extract and cyclophosphamide at IC15 and IC25 concentration resulted in synergism (CI<1). There is potential synergism arise with combination treatment of this plant with cyclophosphamide, which enhances cytotoxicity and the mechanism of synergism worth to be further investigated.

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Cytotoxicity and cytochrome P450 inhibitory activities of Clinacanthus nutans

Cited by 14 publications

References 22 publications

Recent Advancement in Anticancer Activity of Clinacanthus nutans (Burm. f.) Lindau

Recent Advancement in Anticancer Activity of Clinacanthus nutans (Burm. f.) Lindau

Antagonistic Drug-herb Interactions between Clinacanthus nutans and Cyclophosphamide on WRL 68 Cell Line

Synergistic Interactions between Christia vespertilionis Leaves Extract and Chemotherapy Drug Cyclophosphamide on WRL-68 Cell Line

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