Cytotoxicity and Bioactivity of Calcium Silicate Cements Combined with Niobium Oxide in Different Cell Lines

Mestieri, Letícia Boldrin; Gomes-Cornélio, Ana Lívia; Rodrigues, Érico; Faria, Gisele; Guerreiro-Tanomaru, Juliane Maria; Tanomaru‐Filho, Mário

doi:10.1590/0103-6440201700525

Cited by 20 publications

(19 citation statements)

References 23 publications

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“…Rodr ıguez-Lozano et al Acidic environment and root repair cements International Endodontic Journal, 52, 1196-1209 rather than bismuth oxide and it is Fe-free. This result was consistent with a previous report showing that tantalum oxide promotes physicochemical properties such as adequate radiopacity and calcium hydroxide production (Tanomaru-Filho et al 2017). In addition, the presence of tantalum oxide in bioactive cements stimulated alkaline phosphatase activity and mineralization formation (Quintana et al 2018).…”

Section: Discussionsupporting

confidence: 94%

“…Correlating the cell viability assays with the cell migration data, similar values of cell migration to the control at pH 5.2 were observed, but lower cell migration rates than the control at pH 7.4. Previous studies with other MTAbased cements displayed osteoinductive potential and good biocompatibility when used as root-end filling materials in the medullary bone of rabbits (Chen et al 2015, Mestieri et al 2017. However, the lack of previous reports evaluating the biological properties of NeoMTA-Plus and Endocem-MTA exposed to an acidic environment may be considered a limitation of the present study.…”

Section: Discussionmentioning

confidence: 78%

“…, Mestieri et al . ). However, the lack of previous reports evaluating the biological properties of NeoMTA‐Plus and Endocem‐MTA exposed to an acidic environment may be considered a limitation of the present study.…”

Section: Discussionmentioning

confidence: 97%

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Evaluation of changes in ion release and biological properties of NeoMTA‐Plus and Endocem‐MTA exposed to an acidic environment

et al. 2019

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Aim To analyse in vitro changes in ion release and biological properties of Endocem‐MTA (Maruchi, Wonju, Korea) and NeoMTA‐Plus (Avalon Biomed Inc, Bradenton, FL, USA) exposed to acidic or neutral environment on human dental periodontal ligament stem cells (hPDLSCs). Methodology Cell viability and wound healing assays were performed using eluates of each material. Cell death and changes in phenotype induced by the set endodontic sealer eluates were evaluated through flow cytometry. To evaluate cell attachment to the different materials, hPDLSCs were directly seeded onto the material surfaces and analysed by scanning electron microscopy. The chemical composition of the materials was determined by energy‐dispersive X‐ray (EDX), and ion release was evaluated by inductively coupled plasma‐mass spectrometry. Statistical analysis was performed with analysis of variance and a Bonferroni or Tukey post‐test (α < 0.05). Results The MTT assay revealed non‐cytotoxic effects of NeoMTA‐Plus and Endocem‐MTA at pH 5.2 and 7.4. However, there were minor differences compared with the control, especially at pH 5.2, where both materials were associated with significantly greater cell viability (P < 0.05). In both environments, the materials stimulated hPDLSCs to migrate. hPDLSCs were attached to the bioactive cements, with multiple prolongations proliferated on the surface of the samples. Moreover, there were no changes to cell phenotype or apoptosis/necrosis rates, indicating that the acidic environment did not induce cell death. Prismatic crystalline structures were seen on the surface of the cements exposed to butyric acid and EDX analysis identified a marked peak of Ca2+ from NeoMTA‐Plus and Endocem‐MTA in acidic and physiological environments. Conclusions An acidic environment favoured the release of Ca2+ ions from both bioactive cements, and the cytotoxicity of these bioactive cements was low in both environments studied.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 78%

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Evaluation of changes in ion release and biological properties of NeoMTA‐Plus and Endocem‐MTA exposed to an acidic environment

et al. 2019

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“…ALP activity increased at 3 and 7 days of cells exposure to the calcium silicate cements, as has been demonstrated in previous studies with the Saos‐2 cell line (Mestieri et al . , Tanomaru‐Filho et al . ).…”

Section: Discussionmentioning

confidence: 99%

Cytocompatibility, bioactive potential and antimicrobial activity of an experimental calcium silicate‐based endodontic sealer

et al. 2019

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Aim To evaluate the cytocompatibility, bioactive potential, antimicrobial and antibiofilm activities of an experimental calcium silicate‐based endodontic sealer, in comparison with TotalFill BC Sealer and AH Plus. Methodology Cytocompatibility was assessed by methyltetrazolium (MTT) and neutral red (NR) assays, after exposure of the Saos‐2 cells to the sealer extracts (1 : 2, 1 : 4, 1 : 8, 1 : 16 and 1 : 32 dilutions) for 24 h. The sealers were manipulated and placed in 12‐well culture plates and exposed to ultraviolet light; then, 5 mL of DMEM without serum was added. Cell bioactivity was evaluated by alkaline phosphatase activity (ALP) and Alizarin red staining (ARS). Antimicrobial and antibiofilm activities were evaluated by direct contact test (DCT) on planktonic cells (DCTPC) and modified DCT on biofilm formed in bovine dentine blocks (MDCT). MTT, NR and ALP data were analysed by two‐way anova and Bonferroni tests; ARS data by anova and Tukey's tests; and the microbiological data by Kruskal–Wallis and Dunn tests (α = 0.05). Results The experimental sealer, TotalFill BC and AH Plus were not cytotoxic to Saos‐2, in comparison with the negative control (P > 0.05). Greater ALP was observed after 7 days of exposure of Saos‐2 to AH Plus and the experimental sealer (P < 0.05) when compared to the control. Significantly greater mineralized nodule production was observed for TotalFill BC and the experimental sealer (P < 0.05). In DCTPC, the experimental sealer and TotalFill BC were associated with a significantly greater reduction of E. faecalis (P < 0.05) and eliminated C. albicans. In MDCT, the experimental sealer and TotalFill BC had significantly greater antibiofilm efficacy (P < 0.05). Conclusions The experimental calcium silicate‐based sealer was cytocompatible, bioactive, antimicrobial against E. faecalis and C. albicans and effective against E. faecalis biofilms, with potential for use in root canal treatment.

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“…Several other studies have also shown that the addition of niobium into biomaterials do not result in cytotoxicity. These investigations include tests with different materials, such as fluorapatite glass‐ceramics (Kushwaha et al, ), phosphate glasses (Minghui, Wenchuan, Jun, et al, ), and bioactive glass‐niobium granules (Fernandes et al, 2012) hydroxyapatite bioceramics (Capanema et al, ) and calcium silicate cements with different cell lines (Mestieri et al, ). In one of these assays, human osteogenic cells (SAOS) were incubated with Nb‐doped hydroxyapatite for 72 h and cell viability was tested by means of a MTT assay.…”

Section: Resultsmentioning

confidence: 99%

In vitro and in vivo osteogenic potential of niobium‐doped 45S5 bioactive glass: A comparative study

et al. 2019

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In vitro and in vivo experiments were undertaken to evaluate the solubility, apatite‐forming ability, cytocompatibility, osteostimulation, and osteoinduction for a series of Nb‐containing bioactive glass (BGNb) derived from composition of 45S5 Bioglass. Inductively coupled plasma optical emission spectrometry (ICP‐OES) revealed that the rate at which Na, Ca, Si, P, and Nb species are leached from the glass decrease with the increasing concentration of the niobium oxide. The formation of apatite as a function of time in simulated body fluid was monitored by 31P Magic Angle Spinning (MAS) Nuclear magnetic resonance spectroscopy. Results showed that the bioactive glasses: Bioglass 45S5 (BG45S5) and 1 mol%‐Nb‐containing‐bioactive glass (BGSN1) were able to grow apatite layer on their surfaces within 3 h, while glasses with higher concentrations of Nb2O5 (2.5 and 5 mol%) took at least 12 h. Nb‐substituted glasses were shown to be compatible with bone marrow‐derived mesenchymal stem cells (BMMSCs). Moreover, the bioactive glass with 1 mol% Nb2O5 significantly enhanced cell proliferation after 4 days of treatment. Concentrations of 1 and 2.5 mol% Nb2O5 stimulated osteogenic differentiation of BMMSCs after 21 days of treatment. For the in vivo experiments, trial glass rods were implanted into circular defects in rat tibia in order to evaluate their osteoconductivity and osteostimulation. Two morphometric parameters were analyzed: (a) thickness of new‐formed bone layer and (b) area of new‐formed subperiostal bone. Results showed that BGNb bioactive glass is osteoconductive and osteostimulative. Therefore, these results indicate that Nb‐substituted glass is suitable for biomedical applications.

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Cytotoxicity and Bioactivity of Calcium Silicate Cements Combined with Niobium Oxide in Different Cell Lines

Cited by 20 publications

References 23 publications

Evaluation of changes in ion release and biological properties of NeoMTA‐Plus and Endocem‐MTA exposed to an acidic environment

Evaluation of changes in ion release and biological properties of NeoMTA‐Plus and Endocem‐MTA exposed to an acidic environment

Cytocompatibility, bioactive potential and antimicrobial activity of an experimental calcium silicate‐based endodontic sealer

In vitro and in vivo osteogenic potential of niobium‐doped 45S5 bioactive glass: A comparative study

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