Cytotoxicity and antitumor activity of organometallic copper (II) Nano particles in a chemically induced hepatocellular carcinoma rat model

Abstract

This study determined the selective cytotoxicity and mutagenic potential of novel Schiff base complex nanoparticles of copper salt. Chemotherapeutic study of this complex nanoparticles both invitro and invivo in the treatment of hepatocellular carcinoma induced in rats had been done. The treatment efficacy of the studied complex nanoparticles was evaluated by measuring antioxidant activities against oxidative stress caused by diethyl nitrosamine in liver tissue. The measurements included reduced glutathione content, superoxide dismutase activity, total antioxidant capacity, as well as malondialdehyde level. Liver and kidney function tests were also determined, in addition to the evaluation of serum alpha-fetoprotein. Histopathological studies were also performed for liver, spleen, kidney, lung and testis tissues. Results showed that the metal complex nanoparticles had a high potency in the treatment of hepatocellular carcinoma which induced by diethyl nitrosamine in rats as it ameliorated from the investigated parameters toward normal control animals. These findings were well appreciated with histopathological studies of diethylnitrosamine group treated with the complex nanoparticles. It was found that the complex nanoparticles under study can interact with antioxidant to form cancer-specific proteasome inhibitors and apoptosis inducers in liver cancer cell.