Cytotoxicity and antioxidant activity of 5-(2,4-dimethylbenzyl)pyrrolidin-2-one extracted from marine Streptomyces VITSVK5 spp.

Abstract

The aim of the present study was to evaluate the cytotoxicity and antioxidant activity of 5-(2,4-dimethylbenzyl)pyrrolidin-2-one (DMBPO) extracted from marine Streptomyces VITSVK5 spp. The strain was isolated from sediment samples collected at the Marakkanam coast of Bay of Bengal, India. Systematic screening of isolates for anti-Aspergillus activity resulted in the identification of Streptomyces species designated as Streptomyces VITSVK5 spp. Bioactivity guided extraction and purification yielded a compound 5-(2,4-dimethylbenzyl)pyrrolidin-2-one (DMBPO) and was tested for cytotoxicity and antioxidant activity. The structure of the extracted compound was established by spectroscopic studies and identified as 5-(2,4-dimethylbenzyl)pyrrolidin-2-one (DMBPO). DMBPO exhibited cytotoxic activity on HEP 2 and Hep G2 cell lines with the IC50 value of 2.8 μg/ml and 8.3 μg/ml, respectively, as compared to Vero cell line (22.6). DMBPO showed the hemolytic EC50 value of 288 μg/ml on human erythrocytes. DMBPO treatment showed fewer (31.7%) aberrations, gaps and chromatid breaks as compared to untreated controls (27.8%) of human chromosomes. DMBPO also exhibited significant (44.13% at 5 μg/ml DMBPO) DPPH radical scavenging activity and total antioxidant activity (50.10% at 5 μg/ml DMBPO). The results of this study showed that DMBPO is cytotoxic to cancer cells and possesses antioxidant property.

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“…[15] Assay of hemolytic activity Hemolytic activity of the lead compound was determined as described by Saurav and Kannabiran. [16] Erythrocytes (RBCs) were separated from blood samples collected from human volunteers and used within 24 h. It was washed three times with sterile saline (0.85%) solution. The cells were centrifuged for 10 min at 8000 rpm, and the pellet was collected.…”

“…The effect of bioactive compounds on the erythrocytic membrane stability serves as an index for the assessment of membrane stability. [16] …”

“…The antifungal compound 5-(2, 4-dimethylbenzyl) pyrrolidin-2-one extracted from Streptomyces sp.VITSVK5 exhibited cytotoxicity against normal Vero cell line (IC 50 value of 22.6 µg/mL). [16] Wang et al reported the cytotoxic activity (IC 50 value of 18.2 µg/mL) of novel furan derivative isolated from Streptomyces spp. HS-HY-071.…”

Review on bioactive potential of marine microbes

“…The cytotoxicity and antioxidant activity of 5-(2,4-dimethylbenzyl)pyrrolidin-2-one (DMBPO) extracted from marine Streptomyces VITSVK5 sp. from sediment samples collected from the Marakkanam coast, Bay of Bengal, India was studied (Saurav and Kannabiran, 2012). Anthracyclinones namely 4,6,11-trihydroxy-9-propyltetracene-5,12-dione and 10β-carbomethoxy-7,8,9,10-tetrahydro-4,6,7α,9α,11-pentahydroxy-9-propyltetracene-5,12-dione have cytotoxic activity against the HCT-8 human colon adenocarcinoma cell line, which were isolated from Micromonospora sp.…”

Isolation and characterization of biologically active alkaloids from marine actinobacteria Nocardiopsis sp. NCS1

“…Among the microorganisms, actinobacteria was the most studied group because of their capacity to produce novel bioactive secondary metabolites, notably antibiotics (Blunt and Prinsep, 2006), anti-tumor agents, immunosuppressive agents (Mann, 2001) and enzymes (Berdy, 2005;Newman and Cragg, 2007;Strohl, 2004). They are also capable of producing clinically important alkaloids (Charan et al, 2004;Macherla et al, 2005;Cho et al, 2006), which have significant and reproducible inhibitory activity against human carcinoma cells (Saurav and Kannabiran, 2012). Marine alkaloids predominantly brominated or chlorinated ones have distinct features as compared to alkaloids from terrestrial sources (Rocha et al, 2005).…”