Cytotoxic Triterpenoids from the Leaves of<i>Euphorbia pulcherrima</i>

Smith-Kielland, I.; Jm, Dornish; Ke, Malterud; Hvistendahl, Georg; Rømming, Christian; Oc, Bøckman; Kolsaker, P; Stenstrøm, Yngve; Nordal, Arnold

doi:10.1055/s-2006-957893

Cited by 37 publications

(16 citation statements)

References 4 publications

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“…23,24) In this strain of mice, metabolic abnormalities manifest early during development and are quite severe in young adult animals. Moreover, once established, these metabolic changes are resistant to modulation by caloric restriction or weight reduction compared with other mouse models of obesity-associated insulin resistance and dyslipidemia.…”

Section: Discussionmentioning

confidence: 99%

Identification of Five Phytosterols from Aloe Vera Gel as Anti-diabetic Compounds

Tanaka

Misawa

Ito

et al. 2006

Biological & Pharmaceutical Bulletin

197

128

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Of the more than 360 Aloe species known, Aloe barbadensis MILLER (Aloe vera) is the most widely used. Aloe vera contain two major parts: firstly, leaves containing a high concentration of anthraquinone compounds that have been used throughout the centuries as a cathartic and for medicinal purges; and secondly, a clear gel that has been used as a food and to treat burns and other wounds. 1,2) Several chemical components of the Aloe gel are thought to be responsible for its wound healing and immunostimulatory properties. For example, glycoprotein Aloectin A is reported to have anti-tumor and anti-ulcer effects, 3) and a 29 kDa glycoprotein has been found to increase proliferation of normal human dermal cells. 4) Most of these polysaccharides are glucomannans, mannans, or pectins with a range of molecular weights. A major focus of research has been on the carbohydrate fraction isolated from Aloe gel known as "acemannan," which comprises a polydispersed b-(1,4)-linked acetylated mannan interspersed with O-acetyl groups. 5)As a result of these studies, there have been numerous reports of Aloe having diverse biological activities, including anti-tumor activity, anti-acid activity, 6) tyrosinase inhibiting activity, 7) and antioxidant activity. 8)Two clinical trials are available from the same research group that reported hyperglycemic effects on fasting blood glucose as well as on HbA1c levels 9,10) with Aloe vera gel. Hikino et al. isolated two hyperglycemic polysaccharides from Aloe arborescens at 1985. 11) Beppu et al. separated two different anti-diabetic components from the leaf pulp and leaf skin of the same plant. A previous study made with Kidachi Aloe (Aloe arborescens var. naralensis) in streptozotocin (STZ)-induced diabetic rats confirmed its efficacy through administration, 12) contrary to Koo who reported hyperglycemic effects in diabetic rats in acute phase with a product containing Aloe vera gel. 13)In this study, we sought to determine the constituents of Aloe vera gel extract that normalize hyperglycemia in the diabetic mouse strain BKS.Cg-m ϩ/ϩ Lepr db/J (db/db), which exhibits many of the metabolic disturbances of human type 2 diabetes including hyperglycemia, obesity, and insulin resistance. Hemoglobin A1c (HbA1c), a binding product of glucose and hemoglobin, increases depending on the severity of hyperglycemia in a glucose level-dependent manner, and the level of HbA1c reflects the past blood glucose control conditions over a long period. Therefore, we tried to isolate the bioactive compounds from Aloe vera gel based on their ability to decrease the HbA1c level of db/db mice. Finally, we examined whether phytosterols and fractions isolated from Aloe vera gel play an important role in anti-hyperglycemic activity. MATERIALS AND METHODS General ProcedureMelting points were determined on a micro melting point apparatus (Yanako MP-3) without correction. NMR spectra were recorded using a Varian Unity-500 spectrometer ( C: 125 MHz). Positive APCI-MS was taken with LC-MS 2000 (Shimadzu).Preparation of Ext...

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Section: Discussionmentioning

confidence: 99%

Identification of Five Phytosterols from Aloe Vera Gel as Anti-diabetic Compounds

Tanaka

Misawa

Ito

et al. 2006

Biological & Pharmaceutical Bulletin

197

128

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“…100 ml each). Fractions 1-32 (2 g), subjected to column chromatography over silica gel, yielded mainly 9,19-cycloart-25-ene-3 ,24-diol (33 mg) 11 while benjaminamide (2, 5 mg) was obtained in fractions 33-54 (3 g) eluted with CHCl 3 /MeOH (6:1).…”

Section: Extraction and Isolationmentioning

confidence: 99%

Lutamide, a New Ceramide Isolated from the Leaves of Ficus lutea

Poumale¹

2012

A Search for Antibacterial Agents

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“…7 Many plants of this genus have been used in folk medicine for centuries against various diseases including infections, skin diseases as well as cancer. [8][9][10][11][12][13] The effects of different Euphorbia species on pathogens microorganism have been studied by researchers in different parts of the world. [14][15][16][17][18][19][20][21][22][23][24] Recently Battaloğlu F showed that chemical characterization of Euphorbia macroclada Boiss plant that belongs Euphorbia family and grows in Nigde region of Turkey was examine and two different substances which were of diterpenoids (horminon) and aliphatic alcohol (1-octacosanol) were found.…”

Section: Introductionmentioning

confidence: 99%

An in vitro Study of Cytotoxic Activity of Euphorbia macroclada boiss on Mcf–7 Cells

Taş¹,

Şahin‐Bölükbaşı²,

Cevik³

et al. 2018

IJPER

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Objective: The study was aimed to evaluate the cytotoxic activity of acetone extract of leaves, flower and body of Euphorbia macroclada boiss on human breast cancer cell line (MCF-7). Material: The cells were plated at a cell density of 1x10 5 cells in 96-well plates and grown with DMEM medium containing supplemented with 10% FBS and 1% penicillin. The cells were treated by different concentrations of acetone extract of Euphorbia macroclada boiss (10-1000 µg/mL) during 24, 48 and 72 h. The cytotoxic activities of the tested compounds were determined by cell proliferation analysis using standard (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: After the evaluation of cytotoxicity assay results, it is determined that flower and body parts have a significant cytotoxic effect on MCF-7 breast cancer cell line. The values that obtained reading at 570 nm spectrophotometrically, were analyzed with GraphPad Prism7 and IC 50 growth inhibition values was determined. Conclusion: The results of MTT assay showed that leaves, flower and body significantly reduced % cell viability comparative to the control. It was also shown that body had more growth inhibitory effect on MCF-7 cell compared to the leaves part.

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Cytotoxic Triterpenoids from the Leaves ofEuphorbia pulcherrima

Cited by 37 publications

References 4 publications

Identification of Five Phytosterols from Aloe Vera Gel as Anti-diabetic Compounds

Identification of Five Phytosterols from Aloe Vera Gel as Anti-diabetic Compounds

Lutamide, a New Ceramide Isolated from the Leaves of Ficus lutea

An in vitro Study of Cytotoxic Activity of Euphorbia macroclada boiss on Mcf–7 Cells

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