An in vitro Study of Cytotoxic Activity of Euphorbia macroclada boiss on Mcf–7 Cells

Abstract

Objective: The study was aimed to evaluate the cytotoxic activity of acetone extract of leaves, flower and body of Euphorbia macroclada boiss on human breast cancer cell line (MCF-7). Material: The cells were plated at a cell density of 1x10 5 cells in 96-well plates and grown with DMEM medium containing supplemented with 10% FBS and 1% penicillin. The cells were treated by different concentrations of acetone extract of Euphorbia macroclada boiss (10-1000 µg/mL) during 24, 48 and 72 h. The cytotoxic activities of the tested compounds were determined by cell proliferation analysis using standard (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: After the evaluation of cytotoxicity assay results, it is determined that flower and body parts have a significant cytotoxic effect on MCF-7 breast cancer cell line. The values that obtained reading at 570 nm spectrophotometrically, were analyzed with GraphPad Prism7 and IC 50 growth inhibition values was determined. Conclusion: The results of MTT assay showed that leaves, flower and body significantly reduced % cell viability comparative to the control. It was also shown that body had more growth inhibitory effect on MCF-7 cell compared to the leaves part.

Estrogenic, Antiestrogenic and Antiproliferative Activities of Euphorbia bicolor (Euphorbiaceae) Latex Extracts and Its Phytochemicals

“…It was previously reported that E. hirta methanolic extract significantly inhibited the proliferation of MCF-7 cells at 24 h at the GI 50 value of 25.26 µg/mL [32], much lower than that of E. bicolor latex extract in our study. Acetone extracts of E. macroclada leaves and flowers induced significant cytotoxicity in MCF-7 breast carcinoma [33]. Butanol, hexane or methanol extracts of E. tirucalli stem inhibited the proliferation of MCF-7 and MDA-MB-231 in a concentration dependent manner [19].…”