Cytotoxic Screening and In Vitro Evaluation of Pentadecane Against<i>Leishmania infantum</i>Promastigotes and Amastigotes

Bruno, Federica; Castelli, Germano; Migliazzo, Antonella; Piazza, Maria; Galante, Antonella; Verde, Vincenza Lo; Calderone, Simona; Nucatolo, Giuseppa; Vitale, Fabrizio

doi:10.1645/15-736

Cited by 25 publications

(5 citation statements)

References 20 publications

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“…Pentadecane exhibited antimicrobial activity against Leishmania infantum Promastigotes and Amastigotes parasites, antifungal activity against Fusarium oxysporum and antioxidant activity. 68,80,81 In addition, Hexadecane was present in EARE (2.14%) and EASE (0.37%). Hexadecane shows antimicrobial and antioxidant activity.…”

Section: Fatty Acids and Their Estersmentioning

confidence: 99%

Comparative GC-MS Analysis of Bioactive Phytochemicals from Different Plant Parts and Callus of Leptadenia reticulata Wight and Arn.

Godara¹,

Dulara²,

Barwer³

et al. 2019

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The aim of this study is identification and comparative analysis of bioactive phytochemicals present in methanol and ethyl acetate extracts of different plant organs and callus of Leptadenia reticulata by GC-MS technique. Methodology: The shade dried plant samples (leaves stem and root) and callus produced from leaf of L. reticulata were powdered and then sequentially extracted in methanol and ethyl acetate solvents. Total eight extracts were prepared which were Methanolic Leaf Extract (MLE), Methanolic Stem Extract (MSE), Methanolic Root Extract (MRE), Methanolic Callus Extract (MCE), Ethyl Acetate Leaf Extract (EALE), Ethyl Acetate Root Extract (EARE), Ethyl Acetate Stem Extract (EASE) and Ethyl Acetate Callus Extract (EACE). Then, each of the extracts was further subjected to Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Results: The GC-MS analysis of the eight extracts revealed the presence of 77 different types of high and low molecular weight phytochemicals and bioactive compounds in varying quantities. Some of the phytochemicals detected for first time in L. reticulata are γ-sitosterol, Campesterol, Pristane, Hexahydrofarnesol, Stearic acid, Arachidic acid, Coniferyl alcohol, n-Tetracosanol-1, Ascorbic acid 2,6-dihexadecanoate, (2S,3S)-3,7,4'-Trihydroxy-5-methoxy-6-methylflavanone etc. These chemical compounds are considered biologically active and pharmacologically important. Conclusion: This study gives a detailed comparison of detection and identification of various bioactive phytochemicals from different plant parts (leaves, stem and root) and callus of L. reticulata. This provides a basis for the biological and biochemical characterization of some newly detected biologically and pharmacologically important phytochemical components from this plant.

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Section: Fatty Acids and Their Estersmentioning

confidence: 99%

Comparative GC-MS Analysis of Bioactive Phytochemicals from Different Plant Parts and Callus of Leptadenia reticulata Wight and Arn.

Godara¹,

Dulara²,

Barwer³

et al. 2019

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“…As n -pentadecane is only released during early pupation [ 4 ], but pupal development takes approximately three to four weeks in G. m. morsitans , n -pentadecane would carry no information about the subsequent survival of the pupae. Interestingly, n -pentadecane has antimicrobial properties [ 26 ], and it is conceivable that the exudates from aggregated pupae create a microbicidal microenvironment in which pupae are more likely to develop optimally and survive. While there could, therefore, be strength in numbers, pupal aggregation exceeding a critical density threshold can be detrimental to survival owing to higher predation [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Tsetse flies ( Glossina morsitans morsitans ) choose birthing sites guided by substrate cues with no evidence for a role of pheromones

et al. 2023

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Tsetse flies significantly impact public health and economic development in sub-Saharan African countries by transmitting the fatal disease African trypanosomiasis. Unusually, instead of laying eggs, tsetse birth a single larva that immediately burrows into the soil to pupate. Where the female chooses to larviposit is, therefore, crucial for offspring survival. Previous laboratory studies suggested that a putative larval pheromone, n -pentadecane, attracts gravid female Glossina morsitans morsitans to appropriate larviposition sites. However, this attraction could not be reproduced in field experiments. Here, we resolve this disparity by designing naturalistic laboratory experiments that closely mimic the physical characteristics found in the wild. We show that gravid G. m. morsitans were neither attracted to the putative pheromone nor, interestingly, to pupae placed in the soil. By contrast, females appear to choose larviposition sites based on environmental substrate cues. We conclude that, among the many cues that likely contribute to larviposition choice in nature, substrate features are a main determinant, while we failed to find evidence for a role of pheromones.

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“…Phytol (3,7,11,15-tetramethyl-2hexadecen-1-ol) is a diterpene with different biological activities, including antimicrobial and anticancer activities, and is a precursor of vitamins E and K (McGinty et al, 2010). Pentadecane is a hydrocarbon with anticancer and antimicrobial activities (Bruno et al, 2015); isocaproic acid (4-methyl-pentanoic acid) is a fatty acid with a known antitumor activity (Colombo et al, 2005) in addition to squalene, which is a well-known terpene that has antiviral and anticancer activity and used as a drug for the hepatitis C virus (Vera et al, 2009;Kotelevets et al, 2017;El-Sheekh & Abomohra, 2020).…”

Section: Discussionmentioning

confidence: 99%

In vitro Anticancer Potentialities of Three Egyptian Cyanobacterial Isolates against Breast Adenocarcinoma and Hepatocellular Carcinoma Cell Lines

Mofeed

Deyab

El-Bilawy³

2022

Egypt. J. Bot.

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C YANOBACTERIA represent a promising but not totally explored source of a wide array of secondary metabolites. They also represent a source for the discovery of compounds and drugs. In the present study, three cyanobacterial species, namely, Arthrospira platensis MH285264, Leptolyngbya boryana MH155317, and Leptolyngbya sp. MH285263 were tested for their anticancer capabilities against hepatocellular carcinoma (Hep-G2) and breast adenocarcinoma (MCF-7) cell lines. In order to obtain the highest biomasses of the tested organisms, they were optimized using 8 media recipes. The optimized organisms were tested for their biochemical constituents. The GC-MS of the tested cyanobacterial extracts revealed the presence of a number of bioactive metabolites which were previously known for their anticancer activity. The extracts of A. platensis and L. sp. showed a cytotoxic effect against the Hep-G2 cell line with IC 50 values of 14 and 15μg.mL −1 , respectively. The extracts of A. platensis and L. sp. showed more cytotoxic effects against the MCF-7 cell line with IC 50 values of 12.1 and 13.2µg.mL −1 , respectively. On the basis of the undiluted concentration used in this study (100μg.mL −1 ), the maximum inhibitory percentages compared with the control against the Hep-G2 and MCF-7 cell lines were 73.2% and 91.2%, respectively, by extracts of L. sp. and A. platensis. L. boryana exhibited a low and neglectable anticancer activity against both cell lines. The obtained promising results herald the existence of natural and safe alternatives with a high efficiency extracted from A. platensis and L. sp. for inclusion in the strategies of cancer treatment.

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Cytotoxic Screening and In Vitro Evaluation of Pentadecane AgainstLeishmania infantumPromastigotes and Amastigotes

Cited by 25 publications

References 20 publications

Comparative GC-MS Analysis of Bioactive Phytochemicals from Different Plant Parts and Callus of Leptadenia reticulata Wight and Arn.

Comparative GC-MS Analysis of Bioactive Phytochemicals from Different Plant Parts and Callus of Leptadenia reticulata Wight and Arn.

Tsetse flies ( Glossina morsitans morsitans ) choose birthing sites guided by substrate cues with no evidence for a role of pheromones

In vitro Anticancer Potentialities of Three Egyptian Cyanobacterial Isolates against Breast Adenocarcinoma and Hepatocellular Carcinoma Cell Lines

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