Cytotoxic properties of immunoconjugates containing melittin-like peptide 101 against prostate cancer: in vitro and in vivo studies

Russell, Pamela J.; Hewish, Dean R.; Carter, Teresa; Sterling-Levis, Katy; Ow, Kim; Hattarki, Meghan; Doughty, Larissa; Guthrie, Robin E.; Shapira, Deborah; Molloy, Peter L.; Werkmeister, Jerome A.; Kortt, Alexander A.

doi:10.1007/s00262-003-0457-9

Cited by 76 publications

(44 citation statements)

References 17 publications

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“…The peptides, i.e. melittin from bee venom (Dempsey 1990;Habermann and Jentsch 1967) and NK-2 (Andrä and Leippe 1999), an internal fragment of porcine NK-lysin, are well-characterized and exhibit pronounced antimicrobial and anti-cancer cell activities (Andrä et al 2007;Russell et al 2004;Schröder-Borm et al 2005). Though NK-2 and melittin are both cationic as well as similar in size and structure, they are characterized by different degrees of target selectivity and hydrophobicity.…”

Section: Introductionmentioning

confidence: 99%

Online monitoring of metabolism and morphology of peptide-treated neuroblastoma cancer cells and keratinocytes

Drechsler¹,

Andrä

2011

J Bioenerg Biomembr

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Antimicrobial peptides are promising anti-cancer agents with a unique mode of action. We established the usage of a chip-based sensor to monitor the dynamic interplay between cells on the chip and peptides and compared it with endpoint tests. Human neuroblastoma cancer cells and spontaneously immortalized non-cancer keratinocytes were perfused with representative peptides (NK-2, NK11, and melittin). The sensor system enabled continuous recording of cell layer impedance (adhesion/ confluence), oxygen consumption (respiration) and extracellular acidification (glycolysis) and provided insights in cell damage, stress response and recovery. Cells responded differentially to peptide treatment. During perfusion, peptides accumulated on the cell surface until they reached a critical concentration. Preceding to cell death, melittin triggered glycolysis, suggesting stress response. NK-2 induced no change in energy metabolism, but led to an increase in impedance, i.e. a temporarily altered morphology, which appeared to be an excellent parameter to detect subtle structural changes of cell layers.

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Section: Introductionmentioning

confidence: 99%

Online monitoring of metabolism and morphology of peptide-treated neuroblastoma cancer cells and keratinocytes

Drechsler¹,

Andrä

2011

J Bioenerg Biomembr

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“…Bee venom has interesting pharmacological properties [1] and it is used in treatment of conditions such as arthritis [2], rheumatism, pain [3,4], cancerous tumors [5,6] and skin diseases.…”

Section: Introductionmentioning

confidence: 99%

Inductively coupled plasma mass spectrometry determination of metals in honeybee venom

Kokot

Matysiak

2008

Journal of Pharmaceutical and Biomedical Analysis

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“…Venom is one of the products of honey bee, which is important component in the pharmaceutical industry. It is used in the treatment of various health conditions such as pain (Kim et al, 2003), cancerous tumours ISSN : 0974-9411 (Print), 2231-5209 (Online) All Rights Reserved © Applied and Natural Science Foundation www.ansfoundation.org (Russell, 2004), skin diseases, arthritis and rheumatism (Putz, 2006), and the wide use of apitoxin (honey bee venom) is giving rise to a new branch of medicine referred to as Apitherapy (the medical use of honey bee products). Moreover the presence of some exocrine cells in the distal part of venom sac which is otherwise known to only store the component of Venom gland led to the present study.In the recent reports, Abreu et al (2010) suggested the presence of secretory cells in the proximal part of the reservoir.…”

Section: Introductionmentioning

confidence: 99%

A study on biochemical composition of the sting gland (poison gland) and the reservoir (poison sac) of the dwarf honey bee Apis florea F. workers

Kumar

Devi

2014

JANS

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A study on biochemical composition of the sting gland (poison gland) and the reservoir (poison sac) of the dwarf honey bee Apis florea F. workers Neelima R. Kumar and Anita Devi* Department of Zoology, Panjab University, Chandigarh, INDIA *Corresponding author. E-mail: anniekadian@gmail.com Received: October 26, 2013; Revised received: February 9, 2014; Accepted: April 1, 2014 Abstract: The glands associated with the sting apparatus of worker honey bee Apis florea produce Venom which is known to be composed of a wide spectrum of biomolecules ranging from biogenic amines to peptides and proteins. The Venom apparatus showed the presence of two important associated exocrine glands i.e. Venom gland and Dufors gland. The secretions of both glands are apocrine and are released into the lumen to be stored in the venom sac. The presence of some exocrine cells in the distal part of venom sac which is otherwise known to only store the component of Venom gland led to the present study. The present study that there were considerable differences in the biochemical composition of Venom gland and Venom sac secretions of Apis species The concentration of lipids (Sting gland =1.423±0.0001 and Reservoir = 1.21±0.0067), proteins (Sting gland=0.440±0.0226, Reservoir = 0.390± 0.032), activity of acid phosphatase (Sting gland=112.09±21.100, Reservoir=22.63±1.467) and hexokinase (Sting gland=20.7±4.016, Reservoir=10.66±2.465) was found to be more in case of Venom gland while cholesterol(Sting gland=0.138±0.0161 reservoir=0.324±0.00323), glucose (Sting gland=189±1.31, Reservoir=321±7.19), free amino acids, and activity of alkaline phosphatase (Sting gland=21.03±0.195 Reservoir=22.4±0.685) was more in Venom sac. Glycogen was absent in both Venom gland and Venom sac of Apis species as confirmed by the absence of glucose-6-phosphatase activity. It is established from the present study that Venom sac also secretes various biochemicals and enzymes which are added to the total Venom.

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Cytotoxic properties of immunoconjugates containing melittin-like peptide 101 against prostate cancer: in vitro and in vivo studies

Abstract: Immunoconjugates deliver beneficial effects; further peptide modifications may increase cytotoxicity.

Cited by 76 publications

References 17 publications

Online monitoring of metabolism and morphology of peptide-treated neuroblastoma cancer cells and keratinocytes

Online monitoring of metabolism and morphology of peptide-treated neuroblastoma cancer cells and keratinocytes

Inductively coupled plasma mass spectrometry determination of metals in honeybee venom

A study on biochemical composition of the sting gland (poison gland) and the reservoir (poison sac) of the dwarf honey bee Apis florea F. workers

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