Inductively coupled plasma mass spectrometry determination of metals in honeybee venom

Kokot, Zenon J.; Matysiak, Jan

doi:10.1016/j.jpba.2008.05.033

Cited by 19 publications

(12 citation statements)

References 10 publications

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“…Various analytical techniques for characterizing the properties of bee venom have been described in the literature: UV and IR spectroscopy [15,16], spectrofluorimetry [17], 1 H NMR spectroscopy [18,19], differential scanning calorimetry (DSC) [20], inductively coupled plasma mass spectrometry (ICP-MS) [21], high performance liquid chromatography (HPLC) [10,[22][23][24][25], and high performance capillary electrophoresis (HPCE) [10,11,26]. Separa-0731-7085/$ -see front matter © 2010 Elsevier B.V. All rights reserved.…”

Section: Introductionmentioning

confidence: 99%

Characterization of honeybee venom by MALDI-TOF and nanoESI-QqTOF mass spectrometry

Matysiak

Schmelzer

Neubert

et al. 2011

Journal of Pharmaceutical and Biomedical Analysis

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Section: Introductionmentioning

confidence: 99%

Characterization of honeybee venom by MALDI-TOF and nanoESI-QqTOF mass spectrometry

Matysiak

Schmelzer

Neubert

et al. 2011

Journal of Pharmaceutical and Biomedical Analysis

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“…The analysis of metal contents in honeybee venom showed that this equipment permits achieving very low levels for quantifying of As, Ba, Pb Cd, Sb and Cu. This tool is important when honeybee venom is a recommended treatment for certain diseases in medicine [60]. Whatever the analysis of honey, pollen, or any other product taken from beehives, it is important to note that the ICP-MS requires several steps to be considered before chemical analysis.…”

Section: Methodsmentioning

confidence: 99%

Analytical Procedures for Determining Heavy Metal Contents in Honey: A Bioindicator of Environmental Pollution

Mejías¹,

Garrido²

2017

Honey Analysis

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Metals are pollutant residues detectable in honey and in fact account for most of the inorganic pollutants found in this food product. Metal pollutants can be accumulated through the food chain and, at levels exceeding safe thresholds, can be toxic to humans and even damage physiological functions. During the honey-making process, bees can transport pollutants to the beehive following contact with polluted botanic species or from drinking contaminated water. Detecting very low concentrations is a persisting challenge to accurately measure these elements in honey. Additionally, since honey is a complex organic matrix, treatments are needed prior to applying any classical chemical methods for metal determination, such as inductively coupled plasma and atomic absorption spectroscopy. Therefore, optimal results are dependent on adequate sample conditioning prior to heavy metal content analyses. Chemical pretreatments include calcination processes and/or acid digestion. Regarding execution, the last steps of any metal detection methodology are the primary determinants of result quality, where any loss of mass is reflected by unreliable values.

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“…A powerful new method without the disadvantages of isotopic labeling methods is inductively coupled plasma (ICP)-MS. This technique, which has also been used to measure the concentration of metal ions in venoms, [119][120][121] has recently been adapted to measure absolute toxin concentrations by detection of sulfur atoms. [114,122] A major advantage of this technique is that it does not require fragmentation or sample preparation, and a single, sulfur-containing standard can be compared against multiple toxin peaks to calculate the concentration of all sulfurcontaining toxins in a venom (on the condition that the primary structures are known).…”

Section: Quantification Of Polypeptide Toxinsmentioning

confidence: 99%

Deadly Proteomes: A Practical Guide to Proteotranscriptomics of Animal Venoms

et al. 2020

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Animal venoms are renowned for their toxicity, biochemical complexity, and as a source of compounds with potential applications in medicine, agriculture, and industry. Polypeptides underlie much of the pharmacology of animal venoms, and elucidating these arsenals of polypeptide toxins—known as the venom proteome or venome—is an important step in venom research. Proteomics is used for the identification of venom toxins, determination of their primary structure including post‐translational modifications, as well as investigations into the physiology underlying their production and delivery. Advances in proteomics and adjacent technologies has led to a recent upsurge in publications reporting venom proteomes. Improved mass spectrometers, better proteomic workflows, and the integration of next‐generation sequencing of venom‐gland transcriptomes and venomous animal genomes allow quicker and more accurate profiling of venom proteomes with greatly reduced starting material. Technologies such as imaging mass spectrometry are revealing additional insights into the mechanism, location, and kinetics of venom toxin production. However, these numerous new developments may be overwhelming for researchers designing venom proteome studies. Here, the field of venom proteomics is reviewed and some practical solutions for simplifying mass spectrometry workflows to study animal venoms are offered.

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Inductively coupled plasma mass spectrometry determination of metals in honeybee venom

Cited by 19 publications

References 10 publications

Characterization of honeybee venom by MALDI-TOF and nanoESI-QqTOF mass spectrometry

Characterization of honeybee venom by MALDI-TOF and nanoESI-QqTOF mass spectrometry

Analytical Procedures for Determining Heavy Metal Contents in Honey: A Bioindicator of Environmental Pollution

Deadly Proteomes: A Practical Guide to Proteotranscriptomics of Animal Venoms

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