Cytotoxic/Proliferative Effects of Umbelliprenin on Colon Cancer Cell Lines

Abstract

Background: Colon carcinoma growth depends on many factors such as, different organisms, and immune cells, which induce and produce inflammatory cytokines. Umbelliprenin is a naturally prenylated coumarin with anti-inflammatory activities. Its ability to induce cancer cell death has shown variation on different cancer cell lines. Objectives: 7-Prenyloxycoumarins, including umbelliprenin have been widely investigated because of their pharmacological activities. This paper shows the effect of umbelliprenin on colon cancer cell lines. Material and Methods: In the present study, invasive SW48 and noninvasive SW1116 were treated with umbelliprenin (6.25, 12.5, 25, 50, 100, and 200 μM), and the cytotoxicity was determined using a methyl thiazolelydiphenyl-tetrazolium bromide (MTT) assay. Results: Umbelliprenin had significant cytotoxic activity against SW48 cells at all study concentrations (except for 6.25 μM), with IC50 values of 117, 77, and 69 μM after 24, 48, and 72 h, respectively. However, it was cytotoxic against SW1116 only at higher concentrations of 100 and 200 μM (34% and 64% cell death). At lower concentrations, umbelliprenin showed a significant proliferative effect on this noninvasive cancer cell line. Our data were validated by eye and microscopic images. Conclusions:We found a moderate cytotoxic activity of umbelliprenin against invasive SW48 cells, and both cytotoxic and proliferative effects on noninvasive SW1116 cells. Therefore, using umbelliprenin as an anti-inflammatory or cytotoxic compound for patients with colon cancer should be used with care.

Assessment of the Antitumor Potential of Umbelliprenin, a Naturally Occurring Sesquiterpene Coumarin

“…In addition, multiple regulatory proteins can be affected upon treatment with UMB. A study suggested down-regulation of many important proteins, i.e., tubulin α-1B, HSP90, HSP27, hnRNP C1/C2, Cyclophilin, and tumor suppressor MST protein, and up-regulation of stathmin and calreticulin in lung cancer cell lines, i.e., QU-DB and A549 [70].…”

Characterization of the Degradation Profile of Umbelliprenin, a Bioactive Prenylated Coumarin of a Ferulago Species

“…1 H NMR spectra were recorded on a Bruker Advance 500 III HD spectrometer at a nominal frequency of 500.11 MHz. 13 C NMR spectra were recorded on a NMR Bruker DRX 400 spectrometer. A Waters CGT Premier mass spectrometer with a chemical ionization source (CI) with methane reagent gas was used for mass spectrometry analysis of degradation products.…”

“…This oxyprenylated coumarin is commonly found in different plant species such as Ferula , Peucedanum , Seseli , Magydaris , and Ammi , but also in some edible fruits and vegetables such as celery ( Apium graveolens L.), wild celery ( Angelica archangelica L.), and Citrus lemon . , Umbelliprenin was shown to exert anti-inflammatory, immunomodulatory, and anticancer activities . A mechanism of action underlying these effects includes inhibition of matrix metalloproteinases and pro-inflammatory enzymes (e.g., lipoxygenase), antioxidant and antigenotoxic properties, chemoprevention of tumors such as papilloma, colorectal, melanoma, and breast carcinomas, induction of apoptosis, abolition of P-glycoprotein-mediated multidrug-resistance, and up-regulation of Mcl-1. − The importance of the in vitro overall stability of drug candidates is nowadays well recognized. − In this context a limited number of studies on coumarins have been reported. The first report dates back to 1957 and has been based merely on the isolation and structure characterization (elemental analysis and mixed melting point) of these compounds .…”

An insight into the role of citrus bioactives in modulation of colon cancer

“…The two different actions may be attributed to the fact that umbelliprenin possesses different apoptotic actions in different cell lines (Barthomeuf, Lim, Iranshahi, & Chollet, 2008). This study emphasised that umbelliprenin can be a future cytotoxic compound but with a simultaneous emphasis on personalised medicinal approach (Hamidinia, Ramezani, & Mojtahedi, 2013). 6,7-dihydroxycoumarin (Esculetin) has also been found to decrease the viability and growth of various human colon cancer cell lines.…”