Cytotoxic potential of few Indian fruit peels through 3-(4,5-dimethylthiazol-yl)-2,5-diphenyltetrazolium bromide assay on HepG2 cells

Garg, Munish; Lata, Kusum; Satija, Saurabh

doi:10.4103/0253-7613.174552

Cited by 22 publications

(7 citation statements)

References 18 publications

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“…Numerous plant-derived active constituents, like, boswellic acid, curcumin, vinca species, Tagetes erecta species and many others have been used to prevent and stop the proliferation of cancerous cells [7,33,[43][44][45][46]. Many studies have demonstrated that the active compounds present in the plants are able to show anticancer activity [47].…”

Section: Anticancermentioning

confidence: 99%

“…Plant-derived medicine is believed to be a safer alternative to conventional medicine without compromising its effectiveness in treatment [2][3][4]. Some plants have a protective effect on human cells, which can minimise the damage caused by the treatment [5][6][7]. However, plant-derived medicine faces challenges in terms of drug delivery and targeted effects with a reduced availability at the desired site.…”

Section: Introductionmentioning

confidence: 99%

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Applications of Nanocarriers as Drug Delivery Vehicles for Active Phytoconstituents

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Chellian

et al. 2020

CPD

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: Many plant-based bioactive compounds have been serving as the origin of drugs since long ago and many of them have been proven to have medicinal value against various chronic diseases, including, cancer, arthritis, hepatic diseases, type 2 diabetes and cardiovascular diseases. However, their clinical applications have been limited due to their poor water solubility, stability, low bioavailability and extensive transformation due to first-pass metabolism. The applications of nanocarriers have been proven to be able to improve the delivery of bioactive phytoconstituents, resulting in the enhancement of various pharmacokinetic properties and thereby, increase the therapeutic value of phytoconstituents. These biocompatible nanocarriers also exert low toxicity to healthy cells. This review focuses on the uses and applications of different types of nanocarriers to enhance the delivery of phytoconstituents for the treatment of various chronic diseases, along with comparisons related to bioavailability and therapeutic efficacy of nano phytoconstituents with native phytoconstituents.

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Section: Anticancermentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Applications of Nanocarriers as Drug Delivery Vehicles for Active Phytoconstituents

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Chellian

et al. 2020

CPD

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“…Other causes may be air pollution, workplace exposure to certain chemicals such as asbestos, nickel, chromium, radioactive gases like radon etc. [48][49][50][51]. The hallmark of lung cancer, like most other cancers, is characterized by abnormal and uncontrolled cell division, which invades nearby tissues, primarily in the lining of air passages [52].…”

Section: Global Prevalence Of Major Respiratory Diseasesmentioning

confidence: 99%

Advancing of Cellular Signaling Pathways in Respiratory Diseases Using Nanocarrier Based Drug Delivery Systems

Mehta

Dhanjal

Satija

et al. 2020

CPD

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: Cell Signaling pathways form an integral part of our existence, that allows the cells to comprehend a stimulus and respond back. Such reactions, to external cues from the environment, are required and are essential to regulate the normal functioning of our body. Abnormalities in the system arise when there are errors developed in these signals, resulting in a complication or a disease. Presently, respiratory diseases contribute to being the third leading cause of morbidity worldwide. According to the current statistics, over 339 million people who are asthmatic, 65 million who are suffering from COPD, 2.3 million who are lung cancer patients and 10 million are tuberculosis patients. This toll of statistics with chronic respiratory diseases leaves a heavy burden on society and nation annual health expenditure. Hence, a better understanding of the processes governing these cellular pathways will enable us to treat and manage these deadly respiratory diseases effectively. Moreover, it is important to comprehend the synergy and interplay of the cellular signaling pathways in respiratory diseases, which will enable us to explore and develop suitable strategies for targeted drug delivery. This review, in particular, focuses on the major respiratory diseases and further provides an in-depth discussion on the various cell signaling pathways that are involved in the pathophysiology of respiratory diseases. Moreover, the review also analyses the defining concepts about advanced nano-drug delivery systems involving various nanocarriers and propose newer prospects to minimize the current challenges faced by researchers and formulation scientists.

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“…Cancer is one of the most dreaded diseases as mortality associated is very high despite the availability of modern treatment modalities [1,2] . Hence, research towards novel, more effective and tumor-specific treatment has gained momentum during the past decades.…”

Section: Abstract: Apoptosis Anticancer Cantharidin Caspase Tamamentioning

confidence: 99%

Tamarindus indica Bark Extract and its Bioactive Fraction Induce Apoptosis in HeLa and PA-1 Cells

Shirisha¹,

Varalakshmi²

2016

pharmaceutical-sciences

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The aim of the current study was to evaluate the anticancer potential of tamarind bark and its bioactive fraction on HeLa and PA-1 cell lines. Tamarind bark extract was prepared with dichloromethane as the solvent using Soxhlet apparatus and treated on HeLa and PA-1 cell lines to determine the anticancer potential by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Further, bioassay guided fractionation of the extract was carried out using thin layer chromatography. In vitro assays like fluorescence microscopy, DNA fragmentation analysis, caspase-9 activity assay and flow cytometry analyses were carried out to determine the mechanism of anticancer activity of the selected bioactive fraction. The fraction was further characterized by gas chromatography-mass spectrometry. Tamarind bark dichloromethane extract was found to be cytotoxic to HeLa and PA-1 cells. Bioassay guided fractionation indicated that the fourth fraction of the extract possessed the bioactive component. Further, in vitro assays demonstrated that the bioactive fraction induced caspase-9-mediated apoptosis in the cells and was able to reduce the total cell count as evidenced by flow cytometry analyses. The gas chromatography-mass spectrometry analysis of the bioactive fraction indicated the presence of cantharidin, an anticancer compound earlier reported from the blister beetles. It can be concluded that the presence of cantharidin might be responsible for inhibition of proliferation and induction of apoptosis in the cancer cells. Key words: Apoptosis, anticancer, cantharidin, caspase, tamarind bark extractCancer is one of the most dreaded diseases as mortality associated is very high despite the availability of modern treatment modalities [1,2] . Hence, research towards novel, more effective and tumor-specific treatment has gained momentum during the past decades. In this pursuit, several medicinal and dietary plants have provided many anticancer compounds till date [3,4] . Tamarindus indica L. commonly known as tamarind is one of the most important dietary plants in which all parts have some nutritional or medicinal value. It has a history that dates back to ancient tribal people who used to prepare decoctions of the parts and use it for medication. It was reported that tamarind bark and leaves were used to treat wounds in West and East Africa [5] . Fruit and leaf have laxative property, paste of dried seeds were used to set fractured bones, and decoction of the bark and leaves were used to treat jaundice [6][7][8] . The bark of tamarind tree was reported as to have antiinflammatory, analgesic and wound healing properties [9,10] . In West Africa, the macerated fresh bark of the young twigs was used both as a purgative and to relieve abdominal pain [11] .Cantharidin is a colorless, odourless terpene secreted by many species of blister beetles. Cantharidin, isolated from the Chinese blister beetles, Mylabris phalerata or M. cichorii, displays antitumor activity and induces apoptosis in many types of tumor cells. It can be poisono...

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Cytotoxic potential of few Indian fruit peels through 3-(4,5-dimethylthiazol-yl)-2,5-diphenyltetrazolium bromide assay on HepG2 cells

Cited by 22 publications

References 18 publications

Applications of Nanocarriers as Drug Delivery Vehicles for Active Phytoconstituents

Applications of Nanocarriers as Drug Delivery Vehicles for Active Phytoconstituents

Advancing of Cellular Signaling Pathways in Respiratory Diseases Using Nanocarrier Based Drug Delivery Systems

Tamarindus indica Bark Extract and its Bioactive Fraction Induce Apoptosis in HeLa and PA-1 Cells

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